Development of bioremediation and of measurement methods of remaining endocrine disrupters in environment.

近年来，包括内分泌干扰物在内的环境污染物被认为是致突变的功能障碍因子，影响胎儿的发育和分化。本研究采用基因工程和分析化学相结合的方法，在已知的血清剥夺诱导细胞凋亡的PC12细胞培养体系的基础上，发现三丁基锡抑制细胞凋亡，壬基酚促进细胞凋亡，从而确定了利用基因工程技术对环境中内分泌干扰物进行生物修复的体系和检测方法。这些现象被认为是由于bax和细胞色素C含量的变化。此外，我们还成功开发了环境中二恶英等内分泌干扰物的便捷测量系统。二恶英的检测范围显示1 fmol至100 pmol。为了构建内分泌干扰物的生物修复系统，预期降解内分泌干扰物的酶的基因和cDNA，已被克隆。在大肠杆菌中表达的酶，表现出降解的内分泌活性。本研究的结果有望为环境中残留内分泌干扰物的生物修复提供参考。

Recently, environmental pollutant including the endocrine disrupters has been considered as dysfunctional factor of mutagenesis, and to affect on development and differentiation of fetal. In this study, to confirm the system of bioremediation and measurement methods of endocrine disrupters in environmental by using of genetic engineering, we applied the methods of genetic engineering and analytical chemistry.In this study, on the basis of PC12 cell cultured system which has been known to be induced apoptosis by serum deprivation, we found that tributyl tin inhibited apoptosis and nonylphenol enhanced apoptosis using the cells. These phenomena were considered to be changed the contents of bax and cytochrome C. In addition, we have successful developed convenience measurement system of endocrine disrupters such as dioxins in environment. The detection range of dioxins using the developed assay system showed 1 fmol to 100 pmol.To construct the system of bioremediation of endocrine disrupters, the genes and cDNAs of enzymes that were expected to degrade the endocrine disrupters, has been cloned. The enzymes expressed in Escherichia coli, showed degraded activity of the endocrine. These results obtained in this study are expected to contribute the establishment of bioremediation of remaining endocrine disrupters in environment.