Development of bioremediation of remaining dioxins using Sea elegans

利用海线虫开发残留二恶英的生物修复技术

• 批准号: 12557033
• 负责人: KURASAKI Masaaki
• 金额: $ 8.38万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557033/
• 关键词: dioxins; bioremediation; reporter assay; Bacillus subtilis; Phanerochaete chrysosporium; DNA microarray; endocrine disrupers; genetic engineering; 測定系の構築; 分解系の構築; CYP1A1転写制御領域; 培養細胞

Recently, environmental pollutant by dioxins, one of the endocrine disrupters has been considered as dysfunctional factor of mutagenesis, and to affect on development and differentiation of fetals. In this study, to confirm the system of measurement and bioremediation of dioxins in environmental by using of genetic engineering.As results, we have successful developed convenience measurement system of dioxins in environment. The detection range of dioxins using the developed reporter assay system showed 10 fmol to 100 pmol. To consruct the system of bioremediation of dioxins, the genes and cDNAs of enzymes that were expected to degrade the dioxins, has been cloned from Bacillus subtilis and Phanerochaete chrysosporium. The enzymes were expressed in Esherichia coli, and the dioxin degraded activity of the expressed enzymes was estimated by using the developed reporter assay above mentioned. From the results, some enzymes showed the activity of degradation of dioxins. These results obtained in this study are expected to contribute the establishment of bioremediation of remaining dioxins in environment.

近年来，环境污染物二恶英（内分泌干扰物之一）被认为是诱变的功能障碍因素，并影响胎儿的发育和分化。本研究旨在利用基因工程技术确定环境中二恶英的测量和生物修复系统。由此，我们成功开发了环境中二恶英的便捷测量系统。使用开发的报告检测系统检测二恶英的范围为 10 fmol 至 100 pmol。为了构建二恶英生物修复体系，从枯草芽孢杆菌和黄孢原毛平革菌中克隆了二恶英降解酶的基因和cDNA。该酶在大肠杆菌中表达，并使用上述开发的报告测定法估计表达酶的二恶英降解活性。结果表明，部分酶表现出降解二恶英的活性。本研究获得的这些结果预计将有助于建立环境中残留二恶英的生物修复方法。

项目成果

Masaaki Kurasaki: "Metals in water in the Central Kalimantan, Indonesia"Bulletin of Environmental Contamination and Toxicology. 65. 591-597 (2000)

Masaaki Kurasaki：“印度尼西亚中加里曼丹水中的金属”环境污染和毒理学通报。

Osamu Yamanoshita: "2,4,5-trichlorophenoxy acetic acid inhibits apoptosis in PC12 cells"Life Sciences. 69. 403-408 (2001)

Osamu Yamanoshita：“2,4,5-三氯苯氧基乙酸抑制 PC12 细胞凋亡”生命科学。

Masaki Ohta: "The effect of trichloethylene on spatio-temporal pattern of LTP in mouse hippocampal slices"International Journal of Neuroscience. 111. 257-271 (2001)

Masaki Ohta：“三氯乙烯对小鼠海马切片 LTP 时空模式的影响”国际神经科学杂志。

Masaaki Kurasaki, Miho Aoki, Tomoe Miura, Sayaka Seki, Shigeru Toriumi, Toshiyuki Hosokawa, Masashi Okabe, Takuya Numata, Shigeru Saito, Takeshi Saito: "A developed method of terminal deoxynucleotidyl transferase system for quantification of DNA damage ca

Masaaki Kurasaki、Miho Aoki、Tomoe Miura、Sayaka Seki、Shigeru Toriumi、Toshiyuki Hosokawa、Masashi Okabe、Takuy​​a Numata、Shigeru Saito、Takeshi Saito：“一种用于定量 DNA 损伤的末端脱氧核苷酸转移酶系统的开发方法

Shigeru Saito, Masaaki Kurasaki, Toshiyuki Hosokawa, Masashi Okabe, Takeshi Saito, Yukiko Fujii, Kazuo Nagashima, Katsumi Yoshida: "Localization of the induced metallothionein and DNA damage in rat kidney after gold injection"Journal of Histochemistry and

Shigeru Saito、Masaaki Kurasaki、Toshiyuki Hosokawa、Masashi Okabe、Takeshi Saito、Yukiko Fujii、Kazuo Nagashima、Katsumi Yoshida：“注射金后大鼠肾脏中诱导的金属硫蛋白和 DNA 损伤的定位”组织化学杂志