Construction of evaluation method of Environmental contaminants for future generation

点击翻译按钮获取中文摘要

The aim of this study is combine of development of novel evaluated methods for endocrine disrupters using cultured cell system and construction of an artificial placenta-like membrane using human Bewo cells. To assess endocrine disrupters as stand points of future generation, effects of endocrine disrupter concentration passed through the membrane like as placenta are evaluated by the developed methods using the cultured cell system.About the novel evaluating system, evaluation systems of endocrine disrupters using reaction of apoptosis and differentiation in the cells were confirmed. In addition, it was successful constructed the evaluation systems of the chemicals on brain function using a reply to LTP and extension of the nerve in brain sections. On the other hand, it was shown that same property concerning pass through ability for the endocrine disrupters was indicated in the constructed placenta-like membrane. And although major part of the chemicals was accumulated in the Bewo cells, effective concentration of them to organism was passed through the membrane. From the results, these combination mentioned above has been indicated as an effective method for evaluation of endocrine disrupters on future generation.