Construction and analysis of the evaluation system of environmental chemical substances on longevity using a longevity gene as an index of assessment

以长寿基因为评价指标的环境化学物质长寿评价体系构建与分析

• 批准号: 23655139
• 负责人: KURASAKI Masaaki
• 金额: $ 2.5万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23655139/
• 关键词: 微量環境物質評価; 影響評価法; 長寿遺伝子; エピジェネチック; リアルタイムPCR; アポトーシス; フタル酸ジエチル; PC12細胞; 微量環境汚染化学物質; サーチュインファミリー

To construct a new evaluation system for environmental contaminants, we have examined effects of chemical contaminants on expression of sirtuin family using PC12 cell system. As results, expression of Sirt 1 was significantly up-regulated by treatment of diethyl phthalate (one of the potential endocrine disrupters and enhance element of apoptosis in the cell), copper or hydrogen peroxide in PC12 cells. In addition, the tendency that expression of Sirt1 and Sirt2 was decreased in the rat offspring from undernourished pregnant mothers was observed. From the results, it was concluded that measurement of Sirt1 and Sirt2 were useful tool for evaluation of chemical substances on organism.

为了构建新的环境污染物评估体系，我们使用PC12细胞系统检测了化学污染物对sirtuin家族表达的影响。结果，在 PC12 细胞中，通过邻苯二甲酸二乙酯（潜在的内分泌干扰物之一，增强细胞凋亡的元素）、铜或过氧化氢处理，Sirt 1 的表达显着上调。此外，在营养不良的怀孕母亲的大鼠后代中观察到Sirt1和Sirt2表达下降的趋势。从结果来看，Sirt1 和 Sirt2 的测量是评估生物体化学物质的有用工具。

项目成果

妊娠期緑茶投与の出産仔のエピゲネチック因子に及ぼす影響

孕期喝绿茶对新生儿表观遗传因素的影响

• 发表时间: 2012
• 作者: 孫永〓;佐藤伸;向井友花;細川敏幸;齋藤健;蔵崎正明
• 通讯作者: 蔵崎正明

Diethyl phthalate enhances expression of SIRT1 and DNMT3a during apoptosis in PC12 cells

• DOI: 10.1002/jat.2816
• 发表时间: 2013-12
• 期刊: Journal of Applied Toxicology
• 影响因子: 3.3
• 作者: Yongkun Sun;Zhikun Guo;S. Iku;T. Saito;M. Kurasaki

Effects of consumption of green tea during lactation on epigenetic factors in female infants of pregnancy rats with low-protein diet

哺乳期饮用绿茶对低蛋白饮食孕鼠雌仔表观遗传因素的影响

• 发表时间: 2012
• 作者: Yongkun Sun;Yuuka Mukai;Shin Sato;Toshiyuki Hosokawa;Takeshi Saito and Masaaki Kurasaki
• 通讯作者: Takeshi Saito and Masaaki Kurasaki

Stevioside enhances apoptosis induced by serum deprivation in PC12 cells

甜菊苷增强 PC12 细胞血清剥夺诱导的细胞凋亡

• DOI: 10.3109/15376516.2012.658978
• 发表时间: 2012
• 期刊: Toxicology Mechanisms and Methods
• 影响因子: 3.2
• 作者: Takahashi K;Sun Y;Yanagiuchi I;Hosokawa T;Saito T;Komori M;Okino T and Kurasaki M
• 通讯作者: Okino T and Kurasaki M

Effects of parabens on apoptosis induced by serum deprivation in PC12 cells

对羟基苯甲酸酯对血清剥夺诱导的 PC12 细胞凋亡的影响

• 发表时间: 2012
• 期刊: Journal of Environmental Science and Health, Part B. Pesticides, Food Contaminants, and Agricultural Wastes
• 作者: Mari Egawa;Kentaro Aoki;Yongkun Sun;Toshiyuki Hosokawa;Takeshi Saito and Masaaki Kurasaki
• 通讯作者: Takeshi Saito and Masaaki Kurasaki