Cell Damage due to Osmotic Stress and Microscale Mass Transfer relative to Cryopreservation
渗透应力和与冷冻保存相关的微量传质引起的细胞损伤
基本信息
- 批准号:15360116
- 负责人:
- 金额:$ 8.64万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In this study, cell damage and death due to the hypertonic electrolyte solution under the unfrozen condition were investigated experimentally in relation to the potential causes of freezing damage of cells. Next, a mathematical model with a form of reaction kinetics was proposed and developed for the cell damage and death.1) The cells from a human prostatic adenocarcinoma cell line (PC-3) were exposed from an isotonic solution (physiological saline) to hypertonic electrolyte solution (NaCl concentration C_m and exposure time τ) back to the isotonic solution under a constant temperature, T℃. Then, the cell membrane integrity was assessed in physiological saline at 25℃ to measure the cell viability. T was set at three values between 4 and 25℃, C_m at four values between 1.8 and 4.0M, and τ at seventeen values between 2 and 170min.2) From the experimental results, survival curve of cell as a function of τ, probability distribution and probability density function of cell death, characteri … More stic exposure times of cell death, etc. were obtained to clarify the dependency of them on τ, C_m, and T.3) The cell viability decreased with increasing exposure time. The decrease in viability was steeper at a higher concentration and higher temperature. When a characteristic exposure time for the decrease in cell viability was defined as exposure time τ_<0.5> at 0.5 in cell viability, the viability versus nondimensional exposure time τ/τ_<0.5> had a similar distribution independently of concentration and temperature. This means that the cell damage and death proceed kinetically and similarly, suggesting that the mechanism may be also similar.4) The cell damage and death process was formulated with a kinetic model of two-step damage on the basis of experimental results of the cell viability. Here, three states of cells : vigorous, damaged but living (medium state), and dead were considered. Four kinds of model were proposed and investigated. A reaction rate constant in the model was determined by the inverse problem analysis and the best model of four was determined. The cell viability was simulated successfully by the analytical solution from the present model. Also, the probability distribution and probability density function of cell death, and reaction kinetic properties were obtained. The reaction kinetic constant increased with an increase in C_m and T. Less
本研究通过实验研究了非冷冻条件下高渗电解质溶液对细胞的损伤和死亡与细胞冷冻损伤的可能原因。1)将人前列腺癌PC-3细胞从等渗溶液(生理盐水)到高渗电解质溶液(氯化钠浓度Cm,暴露时间τ),在恒温T℃条件下,返回到等渗溶液中。然后,在25℃的生理盐水中检测细胞膜的完整性,以测量细胞活力。将T设置为4~25℃之间的3个值,C_m设置为1.8~4.0M之间的4个值,τ设置为2~170分钟之间的17个值。2)从实验结果看,细胞存活曲线与τ、细胞死亡的概率分布和概率密度函数有关,表征了…获得了更多的细胞死亡等不同的暴露时间,以阐明它们与τ、C_m和T的依赖关系。3)细胞活力随着暴露时间的增加而降低。浓度越高,温度越高,活力下降幅度越大。当细胞存活率下降的特征暴露时间定义为细胞存活率为0.5时的暴露时间τ;0.5时,细胞存活率与无量纲暴露时间τ/τ_1;0.5时的分布与浓度和温度无关。这意味着细胞的损伤和死亡是以动力学的方式进行的,这表明其机制也可能是相似的。4)在细胞存活率实验结果的基础上,建立了细胞损伤和死亡过程的两步损伤动力学模型。在这里,细胞的三种状态被考虑:活力、受损但活着(中等状态)和死亡。提出并研究了四种模型。通过反问题分析确定了模型中的一个反应速率常数,确定了四个模型中的最佳模型。用该模型的解析解成功地模拟了细胞的存活率。得到了细胞死亡的概率分布和概率密度函数,以及反应动力学性质。反应动力学常数随C_m和T_s的增大而增大。
项目成果
期刊论文数量(47)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Cell Viability and Kinetic Model for Prediction of Viability under Exposure to Hypertonic NaCl Solution at Near-zero Temperatures (2nd)
用于预测在接近零温度下暴露于高渗 NaCl 溶液时的活力的细胞活力和动力学模型(第二)
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Hiroshi ISHIGURO;et al.
- 通讯作者:et al.
高張電解質水溶液による細胞損傷・死滅の相似的特性(非凍結低温条件)
高渗电解质溶液(非冰冻低温条件)引起细胞损伤和死亡的相似特征
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:石黒 博;他1名
- 通讯作者:他1名
Cell Viability and Kinetic Model for Prediction of Viability under Exposure to Hyperitonic NaCI Solution at Near-zero Temperatures
用于预测在接近零温度下暴露于高渗 NaCl 溶液时的活力的细胞活力和动力学模型
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:Hiroshi ISHIGURO;et al.
- 通讯作者:et al.
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ISHIGURO Hiroshi其他文献
Hierarchical Argumentation Structure for Persuasive Argumentative Dialogue Generation
用于有说服力的论证对话生成的分层论证结构
- DOI:
10.1587/transinf.2019edp7147 - 发表时间:
2020 - 期刊:
- 影响因子:0.7
- 作者:
SAKAI Kazuki;HIGASHINAKA Ryuichiro;YOSHIKAWA Yuichiro;ISHIGURO Hiroshi;TOMITA Junji - 通讯作者:
TOMITA Junji
ISHIGURO Hiroshi的其他文献
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{{ truncateString('ISHIGURO Hiroshi', 18)}}的其他基金
Dynamics of Morphological Changes of Cells and Its High-Accuracy Kinetic Model under High Temperature Related to Thermal Therapy
热疗相关高温下细胞形态变化动力学及其高精度动力学模型
- 批准号:
16H04281 - 财政年份:2016
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on semi-autonomous teleoperated androids that have humanlike presence
对具有类人存在的半自主遥控机器人的研究
- 批准号:
25220004 - 财政年份:2013
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
A study of molecular pathogenesis of chronic pancreatitis by using polycystic kidney rat
多囊肾大鼠慢性胰腺炎分子发病机制研究
- 批准号:
24591010 - 财政年份:2012
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanisms for Ca2+ absorption by pancreatic ductal epithelium : A study looking for a new strategy to prevent pancreatic stone formation.
胰腺导管上皮吸收 Ca2 的机制:一项寻找预防胰腺结石形成新策略的研究。
- 批准号:
21590873 - 财政年份:2009
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Characteristics of Cell Damage and Death under High Temperature Condition and Its Phenomenological Mathematical Model
高温条件下细胞损伤和死亡的特征及其唯象数学模型
- 批准号:
21360098 - 财政年份:2009
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on humanlike presence by using tele-operated androids
使用远程操作机器人研究类人存在
- 批准号:
20220002 - 财政年份:2008
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Standardization of tailor-made chemotherapy based on pharmacokinetics and pharmacogenomics
基于药代动力学和药物基因组学的定制化疗标准化
- 批准号:
19590533 - 财政年份:2007
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Role of CFTR gene expression in the pathogenesis of chronic pancreatitis : regulation by non-coding region
CFTR基因表达在慢性胰腺炎发病机制中的作用:非编码区的调控
- 批准号:
19590758 - 财政年份:2007
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Complex Cell-Damage and Death due to Extracellular Freezing and Its Mathematical Model
细胞外冷冻引起的复杂细胞损伤和死亡及其数学模型
- 批准号:
18360105 - 财政年份:2006
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Studies on intelligent information processing for robots integrated with the sensor networks
传感器网络集成机器人智能信息处理研究
- 批准号:
16200013 - 财政年份:2004
- 资助金额:
$ 8.64万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
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