Mechanisms for Ca2+ absorption by pancreatic ductal epithelium : A study looking for a new strategy to prevent pancreatic stone formation.

胰腺导管上皮吸收 Ca2 的机制：一项寻找预防胰腺结石形成新策略的研究。

• 批准号: 21590873
• 负责人: ISHIGURO Hiroshi
• 金额: $ 2.91万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590873/
• 关键词: 膵石症; 膵導管上皮; カルシウム輸送体; 単離小葉間膵管; microperfusion; 多発性嚢胞腎症; PCKラット; 線毛; 膵導管細胞; 細胞内Ca^<2+>濃度; polycystic kidneyラット; fibrocystin; secretin; 溶液分泌; 膵石; Ca^<2+>チャネル; 膵液; Panc-1細胞

Pancreatic juice is an alkaline fluid that contains digestive enzymes and high concentrations of HCO3-. A little amount of CO32-in the juice binds to acinar cell-derived Ca2+, which leads to the precipitation of CaCO3. Pancreatic ductal epithelium is supposed to absorb luminal Ca2+ to prevent the formation of pancreatic stone. In the present study, we identified the membrane localization of Ca2+ transporters, measured the Ca2+-transport activities, and demonstrated the mechanisms to absorb luminal Ca2+ by pancreatic ductal epithelium.

胰液是一种碱性液体，含有消化酶和高浓度的HCO 3-。果汁中的少量CO 32-与腺泡细胞来源的Ca 2+结合，导致CaCO 3沉淀。胰腺导管上皮细胞可以吸收胰管内的钙离子，从而防止胰管结石的形成。在本研究中，我们确定了膜定位的Ca 2+转运蛋白，测量Ca 2+的运输活动，并证明了机制，通过胰腺导管上皮细胞吸收腔Ca 2+。

项目成果

High glucose inhibits HCO 3 - and fluid secretion in rat pancreatic ducts

• DOI: 10.1007/s00424-009-0731-6
• 发表时间: 2009-11-01
• 期刊: PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
• 影响因子: 4.5
• 作者: Futakuchi, Sachiko;Ishiguro, Hiroshi;Goto, Hidemi
• 通讯作者: Goto, Hidemi

CFTR functions as a bicarbonate channel in pancreatic duct cells.

• DOI: 10.1085/jgp.200810122
• 发表时间: 2009-03
• 期刊: The Journal of general physiology
• 作者: Ishiguro H;Steward MC;Naruse S;Ko SB;Goto H;Case RM;Kondo T;Yamamoto A
• 通讯作者: Yamamoto A

Pancreatic duct dysfunction and

胰管功能障碍和

• 发表时间: 2011
• 作者: 真嶋浩聡;堀江泰夫;大西洋英;Ishiguro H
• 通讯作者: Ishiguro H

Physiology and pathophysiology of bicarbonate secretion by pancreatic duct epithelium.

胰管上皮分泌碳酸氢盐的生理学和病理生理学。

• 发表时间: 2012
• 期刊: Nagoya J Med Sci
• 影响因子: 0.9
• 作者: Ishiguro H;Yamamoto A;Nakakuki M;Yi L;Ishiguro M;Yamaguchi M;Kondo S;Mochimaru Y.
• 通讯作者: Mochimaru Y.

Fluid secretion from the pancreatic duct in PCK rats.

PCK 大鼠胰管的液体分泌。

• 发表时间: 2010
• 作者: Yi L;Ishiguro H;Yamamoto A;Nakauki M;Furuya S;Nagao S;Wei M;Kondo T;Naruse S.
• 通讯作者: Naruse S.