Study on the mechanism of quality deterioration of agricultural products through cell membrane permeability and surface nature of cell wall

通过细胞膜通透性和细胞壁表面性质研究农产品品质劣化机理

• 批准号: 15380170
• 负责人: OSHITA Seiichi
• 金额: $ 9.34万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15380170/
• 关键词: cell membrane water permeability; diffusional water permeability; osmotic water permeability; water loss; proton relaxation time; water exchange time; aquaporin; spinach; 膨圧; 細胞膜の水透過性; ミスティング; 萎れ; 目減り; 細胞膜水透過係数

As indices of water permeability of spinach leaf cells, 2 kinds of water permeability coefficient, diffusional permeability Pd and osmotic permeability Lp were measured and the change in expression of aquaporin PM28A that regulates water movement across membrane with time was also examined.Through the measurement of Pd by NMR method, the water exchange time and diffusional water permeability were determined at the end of 1d and 6d storage under 2℃, The results showed that the water exchange time and the diffusional water permeability varied from 71.3 ms to 55.4 ms and from 5.1x10^<-5> to 6.6x10^<-5> ms^<-1> respectively. This indicated the increase in water permeability of cell membrane as storage time quantitatively.The osmotic water permeability Lp was also measured by newly developed method that makes a protoplast expose to solutions with different osmotic pressure. Lp was determined for the spinach leaf cells stored at 2℃ and 20℃. While Lp increased after at least 72h storage at 20℃, it was kept almost same as the initial value. The increase in Lp is caused by the increase in the expression of aquaporin that accelerates the water transport and/or the change in membrane properties due to loose packing of phospholipid bilayer. As the expression of PM28A, one of aquaporins, measured in this study decreased to nearly half in 2d storage, the deterioration of phospholipid bilayer is considered to be a major cause of the increase in Lp after 72h storage.With the information above, this study gives the clue to suppress water loss in 1 to 2 d after harvest by applying an appropriate stress to agricultural products to decrease the expression of aquaporin.

作为菠菜叶细胞的透水性指标，测定了2种透水性系数，即扩散透性Pd和渗透透性Lp，并研究了调节水跨膜运动的水通道蛋白PM28 A的表达随时间的变化。通过NMR法测定Pd，在2℃下储存1d和6d后测定其水交换时间和扩散透水性，结果表明，水交换时间和扩散透水性分别为71.3 - 55.4ms和5.1 <-5>- 6.6 × 10 ~（-1）<-5>ms ~<-1>（-1）。这表明随着贮存时间的延长，细胞膜透水性增加，渗透透水性Lp也随着贮存时间的延长而增加。测定了2℃和20℃贮藏菠菜叶片细胞的脂蛋白。在20℃下储存72 h后，Lp值有所上升，但仍基本保持在初始值。Lp的增加是由水通道蛋白表达的增加引起的，水通道蛋白的表达加速了水的转运和/或由于磷脂双层的松散堆积而引起的膜性质的变化。由于本研究中测量的水通道蛋白之一PM28 A的表达在储存2d时下降至近一半，因此磷脂双分子层的劣化被认为是储存72 h后Lp升高的主要原因。本研究提供了通过对农产品施加适当的胁迫以降低水通道蛋白。

项目成果

17 beta-estradiol inhibits cardiac fibroblast growth through both subtypes of estrogen receptor.

• DOI: 10.1016/j.bbrc.2003.09.232
• 发表时间: 2003-11
• 期刊: Biochemical and biophysical research communications
• 影响因子: 3.1
• 作者: Tokumitsu Watanabe;M. Akishita;Hong He;Y. Miyahara;K. Nagano;T. Nakaoka;N. Yamashita;K. Kozaki;Y. Ouchi

Dynamic state of water and cell membrane permeability of spinach leaf during storage

菠菜叶贮藏过程中水分和细胞膜透性的动态变化

• 发表时间: 2006
• 期刊: Cryobiology and Cryotechnology 52(1)(印刷中)
• 作者: Tanbo;K.;Seiichi Oshita
• 通讯作者: Seiichi Oshita

Estrogen receptor beta mediates the inhibitory effect of estradiol on vascular smooth muscle cell proliferation.

雌激素受体β介导雌二醇对血管平滑肌细胞增殖的抑制作用。

• 发表时间: 2003
• 期刊: Cardiovasc Res. 59(3)
• 作者: Watanabe T;Ouchi Y et al.
• 通讯作者: Ouchi Y et al.

Change in diffusional water permeability of spinach leaf cell membrane determined by NMR relaxation time

核磁共振弛豫时间测定菠菜叶细胞膜扩散水渗透率的变化

• 发表时间: 2004
• 期刊: AgEng 2004 Conference Engineering the Future (Books of Abstracts) Part 2
• 作者: Oshita;S.;et al.
• 通讯作者: et al.

HMG-CoA reductase inhibitors inhibit human vascular smooth muscle cell calcification…Amother pleiotropic effect of statins.

HMG-CoA还原酶抑制剂抑制人血管平滑肌细胞钙化……他汀类药物的另一种多效作用。

• 发表时间: 2003
• 期刊: Atherosclerosis Supplements 42
• 作者: Son B;Ouchi Y;et al.
• 通讯作者: et al.