Development of measuring device of the hydraulic conductivity of cell membrane and the control of membrane water permeability under stress

细胞膜水力传导率测量装置及应力下膜水渗透率控制的研制

• 批准号: 18380148
• 负责人: OSHITA Seiichi
• 金额: $ 10.72万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18380148/
• 关键词: membrane hydraulic conductivity; cell membrane; spinach; low temperature stress; mesopyll cell; water channel; 細胞膜水透過性; 浸透水透過係数; 水分損失; 画像処理

Knowledge of dynamic state of water and membrane permeability will provide a measure against water loss of leafy vegetables. Therefore, the proton NMR relaxation time T_1 and the osmotic water permeability Lp of spinach cell membrane were measured. T1 of water in spinach leaf was composed of long and short components. The increase in long T_1 suggested the deterioration of cell membrane during storage. This corresponded to the increase in Lp that regulates the water movement across membrane. This finding implies that water changes its site from vacuole to cytoplasm as Lp increases and it can be detected through long T_1.As to the storage at 20℃, Lp of spinach leaf cell did not show any obvious change until 24h. But the Lp increased 48h later. For the Lp of spinach leaf exposed to 2℃ for 24h preliminary to the 20℃ storage, the increase in Lp delayed in comparison with the 20℃ storage. This means the exposure to 2℃ for the first 24h affected on suppression of cell membrance deterioration. On the other hand, the Lp of the spinach leaf stored at 2℃ did not show any obvious change over the storage period. This indicated that the deterioration of the cell membrance was suppressed at 2℃ storage. One of the reason why the weitht loss of samples was suppressed might be due to the maintenance of Lp at the initial level induced by exposure to low temperature. The measuring device developed in this study was almost automated.

了解叶菜类蔬菜水分动态和膜透性，可为防止水分流失提供依据。因此，测定了菠菜细胞膜的质子核磁共振弛豫时间T_1和渗透透水性Lp。菠菜叶片水分T1由长、短组分组成。长T_1值的增加表明贮藏过程中细胞膜的破坏。这对应于调节跨膜水运动的Lp的增加。这一结果表明，随着Lp的增加，水分从液泡向细胞质转移，并可通过长T_1期检测到。在20 ℃贮藏条件下，菠菜叶片细胞的Lp直到24h才出现明显变化。但48 h后Lp升高。菠菜叶片在20 ℃贮藏前先经2 ℃处理24h，其脂蛋白的上升速度比20 ℃贮藏的有所延迟。这表明在2 ℃下暴露24 h对抑制细胞膜劣化有影响。2 ℃贮藏的菠菜叶片Lp在贮藏期间没有明显变化。这表明，在2 ℃下储存抑制了细胞膜的劣化。低温处理可使Lp维持在初始水平，这可能是抑制样品失重的原因之一。在这项研究中开发的测量设备几乎是自动化的。

项目成果

農産物細胞膜の物性、水の動態と水分損失との関係

农产品细胞膜物理性质、水动力学和失水量之间的关系

• 发表时间: 2007
• 作者: 大下誠一;牧野義雄;川越義則
• 通讯作者: 川越義則

ホウレンソウ葉の組織・細胞内水の状態変化

菠菜叶组织和细胞内水状态的变化

• 发表时间: 2007
• 作者: 大下誠一;Himani Chamila Samarakoon;牧野義雄;川越義則
• 通讯作者: 川越義則

The Effect of low temperature exposure on suppression of water loss of Spinach Leaf through Cell Membrane permeability

低温暴露通过细胞膜通透性抑制菠菜叶失水的效果

• 发表时间: 2006
• 期刊: XVI CIGR World Congress, Agricultural Engineering for a Better World, Book of Abstracts
• 作者: D.Ajiro;Y.Nakai;T.Fukumoto;S.Oshita;Y.Kawagoe;Y.Makino
• 通讯作者: Y.Makino

葉菜類の蒸散抑制に関する基礎研究

叶类蔬菜蒸腾抑制的基础研究

• 发表时间: 2006
• 期刊: 農業環境工学関連学会2006年合同大会要旨集(CD) in CD
• 作者: He Liwen;網代大輔;土谷英範;大下誠一;牧野義雄;川越義則
• 通讯作者: 川越義則

Study of the effect of low temperature on cell membrane permeability and suppression of water loss

低温对细胞膜通透性和抑制水分流失影响的研究

• 发表时间: 2006
• 作者: D. Ajiro;Y. Nakai;T. Fukumoto;S. Oshita;Y. Makino;Y. Kawagoe
• 通讯作者: Y. Kawagoe