Role of the implantation-related cell adhesion molecule, trophinin in progression of colorectal cancer.
植入相关细胞粘附分子肌营养蛋白在结直肠癌进展中的作用。
基本信息
- 批准号:15390115
- 负责人:
- 金额:$ 8.77万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:2003
- 资助国家:日本
- 起止时间:2003 至 2005
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Trophinin is a cell adhesion molecule expressed by both trophoblasts and maternal cells, where it is implicated in human embryo implantation through a homophilic mechanism. During the process of implantation, trophoblasts aggressively invade and actively proliferate to form the placenta, a process resembling tumor invasion. In the research project, we first identify human chorionic gonadotrophin (hCG) as a stimulator of trophinin, We then demonstrate that trophinin is expressed in colorectal cancer cells in 59.5% of 42 patients examined, and that its expression is closely associated with poor prognosis. Expression of human chorionic gonadotrophin was associated with trophinin expression in these patients. To determine whether trophinin enhances invasion of cancer cells, colon adenocarcinoma SW480-tro cells stably expressing trophinin were established by transfection, and Matrigel assays showed that these cells were more invasive than mock-transfected cells. A microarray search for transcripts up-regulated in SW480 cells transiently transfected by trophinin cDNA identified the high-mobility group box 1 gene (HMGB1). In addition, the receptor for advanced glycation end products (RAGE), which serves as a ligand of HMGB1, was largely co-expressed with trophinin in colorectal cancer tissue specimens. These results suggest that trophinin enhances cancer cell invasiveness through a mechanism involving HMGB1/RAGE and that its expression indicates a poor prognosis in colorectal cancer.
肌营养蛋白是一种由滋养层细胞和母体细胞表达的细胞粘附分子,通过同源机制参与人类胚胎植入。在着床过程中,滋养层细胞积极侵袭并活跃增殖形成胎盘,这一过程类似于肿瘤的侵袭。在该研究项目中,我们首先确定了人绒毛膜促性腺激素(hCG)作为肌营养蛋白的刺激剂,然后我们证明肌营养蛋白在所检查的42名患者中59.5%的结直肠癌细胞中表达,并且其表达与不良预后密切相关。这些患者中人绒毛膜促性腺激素的表达与肌营养蛋白的表达相关。为了确定肌钙蛋白是否增强癌细胞的侵袭性,通过转染建立稳定表达肌钙蛋白的结肠腺癌SW480-tro细胞,Matrigel检测显示这些细胞比模拟转染的细胞更具侵袭性。微阵列搜索在瞬时转染营养蛋白 cDNA 的 SW480 细胞中上调的转录本,鉴定出高迁移率组框 1 基因 (HMGB1)。此外,作为 HMGB1 配体的晚期糖基化终末产物 (RAGE) 受体在结直肠癌组织标本中很大程度上与肌营养蛋白共表达。这些结果表明,营养蛋白通过涉及 HMGB1/RAGE 的机制增强癌细胞的侵袭性,并且其表达表明结直肠癌的预后不良。
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Female adnexal tumor of probable wolffian origin : A morphological, immunohistochemical, and ultrastructural study with c-kit gene analysis
可能起源于沃尔夫氏菌的女性附件肿瘤:利用 c-kit 基因分析进行形态学、免疫组织化学和超微结构研究
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Harada O;et al.
- 通讯作者:et al.
Expression of core 2 β1,6-N-acetylglucosaminyltransferase facilitates prostate cancer progression
- DOI:10.1093/glycob/cwi086
- 发表时间:2005-10-01
- 期刊:
- 影响因子:4.3
- 作者:Hagisawa, S;Ohyama, C;Fukuda, M
- 通讯作者:Fukuda, M
Malignant myoepithelioma (myoepithelial carcinoma) of soft tissue
- DOI:10.1111/j.1440-1827.2005.01861.x
- 发表时间:2005-08-01
- 期刊:
- 影响因子:2.2
- 作者:Harada, O;Ota, H;Nakayama, J
- 通讯作者:Nakayama, J
Implantation-dependent expression of trophinin by maternal fallopian tube epithelia during tubal pregnancies - Possible role of human chorionic gonadotrophin on ectopic pregnancy
- DOI:10.1016/s0002-9440(10)63579-1
- 发表时间:2003-12-01
- 期刊:
- 影响因子:6
- 作者:Nakayama, J;Aoki, D;Fukuda, MN
- 通讯作者:Fukuda, MN
Hypermethylation of ASC/TMS1 is a sputum marker for late-stage lung cancer
- DOI:10.1158/0008-5472.can-05-4447
- 发表时间:2006-06-15
- 期刊:
- 影响因子:11.2
- 作者:Machida, Emi Ota;Brock, Malcolm V.;Baylin, Stephen B.
- 通讯作者:Baylin, Stephen B.
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{{ truncateString('NAKAYAMA Jun', 18)}}的其他基金
Role of IL-33 on gastric cancer development -Analysis using new model mice-
IL-33对胃癌发展的作用-使用新模型小鼠进行分析-
- 批准号:
25670191 - 财政年份:2013
- 资助金额:
$ 8.77万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
Regulation of gastric cancer development by mucin-type glycans
粘蛋白型聚糖对胃癌发展的调节
- 批准号:
24390086 - 财政年份:2012
- 资助金额:
$ 8.77万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of single nucleotide polymorphisms of glycosyltransferase gene responsible for the biosysnthesis of gastricο-glycans and its significance for genetic factor involved in the development of gastric diseases
胃聚糖生物合成糖基转移酶基因单核苷酸多态性分析及其对胃病发生遗传因素的意义
- 批准号:
21390104 - 财政年份:2009
- 资助金额:
$ 8.77万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Functional analysis of chondroitin sulfate E expressed in glioma
硫酸软骨素E在胶质瘤中表达的功能分析
- 批准号:
18390113 - 财政年份:2006
- 资助金额:
$ 8.77万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Regulation of Helicobacter pylori by O-glycan
O-聚糖对幽门螺杆菌的调节
- 批准号:
14082201 - 财政年份:2002
- 资助金额:
$ 8.77万 - 项目类别:
Grant-in-Aid for Scientific Research on Priority Areas
Molecular analysis of acute rejection on transplanted liver
移植肝急性排斥反应的分子分析
- 批准号:
12670197 - 财政年份:2000
- 资助金额:
$ 8.77万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular Pathology of Glycosyltransferases Involved in the Progression of Colorectal Cancer
糖基转移酶参与结直肠癌进展的分子病理学
- 批准号:
09670222 - 财政年份:1997
- 资助金额:
$ 8.77万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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