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Human papilloma virus in lung and esophageal carcinoma. Molecular pathology of Japan and Asia

肺癌和食道癌中的人乳头瘤病毒。

基本信息

批准号：
15406011
负责人：
FUKAYAMA Masashi
金额：
$ 8.64万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15406011/
关键词：
human papilloma virus lung carcinoma esophageal carcinoma molecular pathology Japan Asia In situ hbridization PCR パラフィンブロック

项目摘要

Human papilloma virus (HPV) is causally associated with carcinoma of the uterine cervix, genital organs, and anus. On the other hand, its relation to the lung and esophageal carcinomas has not been clarified yet, since the detection rates of HPV have been variable in the institutions and regions. In the present study, we investigated the HPV infection in lung and esophageal carcinomas in Japan and Asian countries in order to clarify the significance of HPV infection and to evaluate the regional differences.In situ hybridization and PCR detection of HPV-DNA were applied to the formalin-fixed and paraffin-embedded specimens of lung and esophageal carcinomas (total 483 samples), which were collected from four institutions of Japan (Tokyo, Kochi, Kagoshima and Okinawa), two institutions of Korea and each of Taiwan and Singapore.The presence of HPV-DNA was demonstrated in low frequencies, such as 6.9% of squamous cell carcinomas of the lung (12/174), 7.1% of adenocarcinomas of the lung (9/127) and 8.8% of esophageal carcinomas (16/182). The positive signals for HPV were sparsely distributed in some of the nuclei of the carcinoma cells in positive cases. The finding suggests that this sparse distribution is one of causes for the variability of the frequencies of the HPV infection in the previous reports.The positive frequencies in lung squamous cell carcinomas were 8.1, 8.6, 5.6 and 0% in Japan, Korea, Taiwan, and Singapore, respectively. The frequencies in lung adenocarcinomas were 5.0, 7.1, 13.3 and 0%, respectively. In esophageal carcinoma, the positive rates were 10.5, 8.0 and 6.4% in Japan, Korea, and Taiwan, respectively.When the frequencies were compared among the regions in Japan, the highest frequency was observed in that of esophageal carcinomas of Kagoshima (24.1%, 7/29), which was statistically significant. Otherwise, there was no regional difference in lung carcinomas.

人乳头状瘤病毒（HPV）与子宫颈癌、生殖器官癌和肛门癌有因果关系。另一方面，其与肺癌和食管癌的关系尚未阐明，因为HPV的检出率在机构和地区之间存在差异。本研究采用原位杂交和PCR技术对日本和亚洲国家肺癌和食管癌石蜡包埋标本进行HPV-DNA的检测，以阐明HPV感染的意义并评价其地区差异（共483份样品），从日本的四个机构收集（东京、高知、鹿儿岛和冲绳），韩国的两个机构以及台湾和新加坡的每个机构。如6.9%的肺鳞状细胞癌（12/174）、7.1%的肺腺癌（9/127）和8.8%的食管癌（16/182）。HPV阳性信号散在分布于部分癌细胞核内。日本、韩国、台湾和新加坡肺鳞癌中HPV阳性率分别为8.1%、8.6%、5.6%和0%。在肺腺癌中的频率分别为5.0%、7.1%、13.3%和0%。日本、韩国和台湾食管癌的阳性率分别为10.5%、8.0%和6.4%，比较日本各地区的阳性率，鹿儿岛食管癌的阳性率最高（24.1%，7/29），有统计学意义。此外，肺癌无地区差异。