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歯周病原性細菌の宿主細胞内侵入と歯周組織破壊機構の分子生物学的解析

牙周病原菌侵入宿主细胞及破坏牙周组织机制的分子生物学分析

基本信息

批准号：
15791040
负责人：
中川一路
金额：
$ 2.05万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Young Scientists (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

歯周病は,複数の細菌により引き起こされる感染性の疾患である.中でも,グラム陰性偏性嫌気性桿菌であるPorphyromonas gingivalisが,歯周疾患の主たる病原菌であることは,多くの研究者の一致する見解である.P.gingivalisは,菌株により異なる病原性を示すと推測されていたが,臨床的ならびに実験的証拠に乏しかった.申請者は,P.gingivalisの宿主への定着・付着にもっとも関与すると考えられている線毛をコードする線毛遺伝子(fimA)が,5型に分類され,2型線毛を有するP.gingivalisが歯周病の進行度・重篤度と強い相関を示すことを明らかとした.細胞内侵入性を示す病原性細菌の多くは,エンドソーム内に封入され,細胞内で消化される.近年,一部の病原細菌がエンドソームを経ずに直接細胞内質に取り込まれるようなZipperモデルも提唱されている.本研究では,この2型線毛を有する菌の細胞への付着・定着機構を明らかとするため,インテグリン強発現細胞株を樹立して線毛タンパクとの結合について解析した.α5β1インテグリン(フィブロネクチンレセプター)あるいはαvβ3インテグリン(ビトロネクチンレセプター)を強発現したCHO細胞に精製したリコンビナント線毛あるいは菌体の付着を確認した.その結果,本菌の宿主細胞への付着は,線毛とフィブロネクチンを介した結合だけでなく,線毛とインテグリン鎖直接の結合が認められた.

Weekly illness, complex disease, bacterial infection, infectious disease, infectious disease. In the middle of the disease, the sex-biased bacteria were infected with Porphyromonas gingivalis, the disease was caused by pathogens, and the researchers were consistent in understanding the pathogenicity of the bacteria. P. gingivalis, the pathogenicity of the strains showed that the pathogenicity of the bacteria was pushed, and the pathogenicity of the patients in the bed was poor. The applicant, the P.gingivalis host, is determined to make sure that the information is correct and that the data are classified as fimA, type 5, and type 2 as well as the severity of P.gingivalis disease and the severity of disease. The invasiveness of the cells showed that the pathogenic bacteria were numerous, and the pathogens were sealed and digested. In recent years, there has been a study of pathogens, including bacteria, bacteria In this study, there are fungus bacteria in the hair of type 2, and the organization is required to make sure that it is clear that it is not safe to do so. The whole cell line was analyzed by the combination of microarray and microsomal analysis. The results showed that the cell growth rate was higher than that of the control group. The results showed that the cell growth rate of the cell line was significantly higher than that of the CHO cell line. The results showed that the host cell of this strain was responsible for the treatment of the bacteria. The results showed that the host cells of the bacteria were responsible for the treatment of the bacteria, and the results showed that the host cells of the bacteria were paid by the host cells, and the bacteria were introduced by the host cells.