Hierarchical Correlation Dynamics among Cellular Rhythms: Coordination and Dissociation of Non-linear Biological Oscillators.

细胞节律之间的层次相关动力学：非线性生物振荡器的协调和分离。

基本信息

批准号：
16300145
负责人：
KAWAHARA Koichi
金额：
$ 9.6万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

There are many rhythmic phenomena in the biological system. A variety of oscillatory phenomena such as a cyclic change in the concentration of intracellular Ca2+ are also observed in the cellular level. The present study aims at elucidating the mechanisms responsible for interactions among cellular oscillatory rhythms, and for the coordination and dissociation of such cellular rhythms. The results are summarized as follows:1. In cultured cardiac myocytes at 4 DIV, the cyclic changes in the concentration of intracellular free Ca2+ (Ca2+ oscillation) in association with spontaneous rhythmic contractions were synchronized among myocytes.2. The intracellular Ca2+ oscillations were also synchronized among cultured cardiac myocytes without apparent physical contact with each other.3. The synchronization of Ca2+ oscillation was persisted even when gap junctional intercellular communication was blocked by treatment with either DSA or heptanol, blockers of gap junction channels. The synchronization was also persisted when the rhythmic contraction of cardiac myocytes was almost perfectly terminated by treatment with BDM, an uncoupler of E-C coupling.4. Treatment of cultured cardiac myocytes with suramin, a blocker of P2-purinoceptors, resulted in the asynchronization of intracellular Ca2+ oscillations among myocytes.5. Taken together, above findings have led to a suggestion that the extracellular ATP-purinoceptor signaling system is involved in the synchronization of intracellular Ca2+ oscillations among cultured cardiac myocytes.6. In addition, analysis using cardiac myocytes-fibroblast co-cultures demonstrated that cardiac fibroblasts co-cultured with cardiac myocytes enhanced the intercellular communication among myocytes via gap junctions, thereby stabilizing the spontaneous contraction rhythm of cultured cardiac mvocvtes.

生物系统中有许多节奏现象。在细胞水平上还观察到了各种振荡现象，例如细胞内Ca2+浓度的循环变化。本研究旨在阐明负责细胞振荡节奏之间相互作用的机制，以及这种细胞节律的协调和解离。结果总结如下：1。在4 DIV的培养心肌细胞中，在肌细胞之间同步了细胞内游离Ca2+（Ca2+振荡）浓度的环状变化。2。细胞内Ca2+振荡也在培养的心肌细胞中同步而没有明显的物理接触。3。即使用DSA或Heptanol（GAP连接通道的阻滞剂）处理间隙连接界面间通信，CA2+振荡的同步也持续存在。当心肌细胞的节奏收缩几乎被用BDM处理（E-C耦合的解偶子）处理时，同步也持续了。4。用Suramin（P2-Purinoceptor的阻断剂）治疗培养的心肌细胞，导致肌细胞之间细胞内Ca2+振荡的异步。5。综上所述，上面的发现导致了一个建议，即细胞外purinoceptor信号传导系统参与培养的心肌细胞中细胞内Ca2+振荡的同步。6。此外，使用心肌细胞肌细胞共培养的分析表明，与心肌细胞共同培养的心脏成纤维细胞通过间隙连接增强了肌细胞之间的细胞间交流，从而稳定了培养的cardiac Mvocvtes的自发性节奏。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Ruthenium red-induced changes in myocardial energetics during normal beat and ventricular fibrillation in isolated rat hearts.

钌红诱导离体大鼠心脏正常搏动和心室颤动期间心肌能量的变化。

DOI：
发表时间：
2005
期刊：
The Cardiology 1(2)
影响因子：
0
作者：
Shiga H.;et al.;Kawahara K. et al.
通讯作者：
Kawahara K. et al.

Coupling between contraction rhythm and calcium oscillation in a cultured cardiac myocyte : Fluctuation behaviour and its modeling.

培养的心肌细胞中收缩节律与钙振荡之间的耦合：波动行为及其建模。

DOI：
发表时间：
2006
期刊：
Biological Rhythm Research 37(2)
影响因子：
0
作者：
Kudo;T.;Kaneko;M.;Iwasaki;H.;Togayachi;A.;Nishihara;S.;Abe;K.;et al.;Nakayama Y. et al.;Yoneyama M. et al.
通讯作者：
Yoneyama M. et al.

Spectral and correlation analyses of the verapamil-induced conversion of ventricular fibrillation to tachycardia in isolated rat hearts.

维拉帕米诱导的离体大鼠心脏室颤向心动过速转化的频谱和相关性分析。