Coordination and dissociation of nonlinear oscillations in cardiomyocytes : dysfunction of holonic oscillators leading to arrhythmias

心肌细胞非线性振荡的协调和解离：完整振荡器功能障碍导致心律失常

• 批准号: 19300153
• 负责人: KAWAHARA Koichi
• 金额: $ 12.06万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2007
• 资助国家: 日本
• 起止时间: 2007 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19300153/
• 关键词: 心筋細胞; 細胞内Ca^<2+>振動; ATP-purinoceptor系; 細胞外ATP振動; 細胞内Mg^<2+>振動; 非線形振動子; 負帰還制御系; ATP-purinocetor系

We have recently found that the extracellular ATP-purinoceptor signaling is crucially involved in the intercellular synchronization of intracellular Ca^<2+> oscillations among cardiomyocytes. In this study, I aim at elucidating whether and how the concentrations of extracellular ATP and intracellular Mg^<2+> oscillate in association with rhythmic contraction of cultured cardiomyocytes from neonatal rats. The simultaneous measurement of the fluo-4 fluorescence and the luciferin/luciferase chemical luminescence showed that the concentration of extracellular ATP oscillated synchronously with the concentration of intracellular Ca^<2+> in cardiomyocytes, supporting our previous inference that the extracellular ATP-purinoceptor signaling is responsible for the intercellular synchronization of intracellular Ca^<2+> oscillations. In addition, the concentration of intracellular Mg^<2+> also oscillated in association with rhythmic contraction and intracellular Ca^<2+> oscillations, suggesting that the concentration of intracellular ATP varied depending on the rhythmic contraction of cardiomyocytes. All these findings have led to an idea that the coordination of multiple nonlinear oscillations in cardiomyocytes such as rhythmic variations in the concentrations of intracellular Ca^<2+> Mg^<2+> and ATP seems critical to the normal function of the heart.

我们最近发现，细胞外purinoceptor信号传导与心肌细胞之间细胞内Ca^<2+>振荡的细胞间同步至关重要。在这项研究中，我旨在阐明细胞外ATP和细胞内Mg^<2+>振荡的浓度与新生大鼠培养的心肌细胞的节奏收缩相关。氟-4荧光和荧光素/荧光素酶化学发光的同时测量表明，细胞外ATP的浓度与细胞内ca^<2+>的浓度同步振荡，在心肌细胞中的细胞内CA^<2+>支持我们先前的构造构成细胞外的构造的构造的构造的构造的构造，从Ca^<2+>振荡。另外，细胞内Mg^<2+>的浓度也与节奏收缩和细胞内Ca^<2+>振荡相关，这表明细胞内ATP的浓度取决于心肌细胞的节奏收缩。所有这些发现都导致了一个想法，即在心肌细胞中多种非线性振荡的协调，例如在细胞内Ca^<2+> mg^<2+>和ATP浓度和ATP浓度中的节奏变化和ATP似乎对心脏正常功能至关重要。

项目成果

Possible Involvement of Extracellular ATP-P2Y Purinoceptor Signaling in Ischemia-induced Tolerance of Astrocytes in Culture

• DOI: 10.1007/s11064-009-9942-7
• 发表时间: 2009-09-01
• 期刊: NEUROCHEMICAL RESEARCH
• 影响因子: 4.4
• 作者: Iwabuchi, Sadahiro;Kawahara, Koichi
• 通讯作者: Kawahara, Koichi

Maintenance and characterization of spontaneous contraction rhythm in cultured cardiac myocytes fused withcardiac fibroblasts

与心肌成纤维细胞融合的培养心肌细胞自发收缩节律的维持和表征

• 发表时间: 2008
• 期刊: BioSystems 92
• 作者: Kawahara;K.;Sato;R.;Matsuyama;D.;Iwabuchi;S;Tatsuro Kosugi;Daisuke Matsuyama
• 通讯作者: Daisuke Matsuyama

Possible involvement of nitric oxide in the suppression of the release of cellular ATP during ischemia in cultured cardiac myocytes

一氧化氮可能参与抑制培养心肌细胞缺血期间细胞 ATP 的释放

• 发表时间: 2007
• 期刊: New Horizons in Coronary Artery Disease
• 作者: Kawahara;K.;Hachiro;T.;Matsuyama;D.
• 通讯作者: D.

The number distribution of complex shear modulus of single cells measured by atomic force microscopy

• DOI: 10.1016/j.ultramic.2009.03.008
• 发表时间: 2009-07-01
• 期刊: ULTRAMICROSCOPY
• 影响因子: 2.2
• 作者: Hiratsuka, Shinichiro;Mizutani, Yusuke;Okajima, Takaharu
• 通讯作者: Okajima, Takaharu

Proliferation of neonatal cardiomyocytes by connexin43 knockdown via synergistic inactivation of p38 MAPK and increased expression of FGF1

• DOI: 10.1007/s00395-009-0029-z
• 发表时间: 2009-11-01
• 期刊: BASIC RESEARCH IN CARDIOLOGY
• 影响因子: 9.5
• 作者: Matsuyama, Daisuke;Kawahara, Koichi
• 通讯作者: Kawahara, Koichi