Effect of US stimulation on adiponectin secretion by adipocytes of human subjects

超声刺激对人类受试者脂肪细胞脂联素分泌的影响

• 批准号: 16300175
• 负责人: TANIGUCHI Nobuyuki
• 金额: $ 5.95万
• 依托单位: JICHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16300175/
• 关键词: Therapeutic ultrasound; Obesity; Human adipocyte; In vitro; ELISA; Adiponectin

To clarify the effect of the repeated sonication on the adiponectin secretion by adipocytes obtained from obese subjects. Using 1-MHz continuous-wave ultrasound at an intensity of 0.50 or 2.1W/cm^2, we sonicated culture flasks of subcutaneous adipocytes obtained from obese human subjects, in a series of 3 sessions of US stimulation applied for a daily total of 15min. For the measurement of adiponectin secretion, 50μl of the culture medium was collected from each flask every 24h after the 1st stimulation. Quantification of adiponectin protein levels in cell culture supernatants was performed with a commercially available ELISA kit recommended by the manufacturer. The adiponectin concentrations in the culture medium of the US stimulation groups rose significantly (p<0.05). Repeated US stimulation may accelerate adiponectin secretion in obese human adipocytes.

阐明重复超声处理对肥胖受试者脂肪细胞分泌脂联素的影响。使用1 MHz的连续波超声，强度为0.50或2.1 W/cm ^2，我们对从肥胖人类受试者中获得的皮下脂肪细胞的培养瓶进行超声处理，每天施加3次US刺激，总共15分钟。为了测量脂联素分泌，在第1次刺激后每24小时从每个烧瓶收集50μl培养基。用生产商推荐的市售ELISA试剂盒定量细胞培养上清液中的脂联素蛋白水平。超声刺激组培养液中脂联素浓度显著升高（p<0.05）。重复超声刺激可加速肥胖人脂肪细胞的脂联素分泌。