Effect of US stimulation on adiponectin secretion by adipocytes of human subjects

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To clarify the effect of the repeated sonication on the adiponectin secretion by adipocytes obtained from obese subjects. Using 1-MHz continuous-wave ultrasound at an intensity of 0.50 or 2.1W/cm^2, we sonicated culture flasks of subcutaneous adipocytes obtained from obese human subjects, in a series of 3 sessions of US stimulation applied for a daily total of 15min. For the measurement of adiponectin secretion, 50μl of the culture medium was collected from each flask every 24h after the 1st stimulation. Quantification of adiponectin protein levels in cell culture supernatants was performed with a commercially available ELISA kit recommended by the manufacturer. The adiponectin concentrations in the culture medium of the US stimulation groups rose significantly (p<0.05). Repeated US stimulation may accelerate adiponectin secretion in obese human adipocytes.