Isolation and Structure Elucidation of Antioxidants of Traditional Vegetables and Fruits of Yaeyama Islands in Okinawa.

我们的目的是寻找和澄清可能支持冲绳人健康和长寿的化学成分，特别是天然抗氧化剂。抗氧化剂不仅在食品保存方面发挥着重要作用，而且在降低大多数心血管疾病、癌症和衰老的风险方面也发挥着重要作用。在对冲绳传统食用植物的抗氧化活性进行筛选的基础上，我们将注意力集中在两种植物上，如日本长胡椒(Piper Retrafium)和诺丽(Morinda Citrifolia)。用OSI法和DPPH自由基清除试验等方法对两种植物的抗氧化活性进行了评价。从裂后黄连提取物的乙酸乙酯可溶部分中分离并测定了几种抗氧化多酚类化合物，其中包括黄酮苷。从柠檬叶中分离鉴定出8个化合物，分别为槲皮素苷、环烯醚萜和阿魏酸苷。

It is our purpose to find and clarify of chemical constituents, in particularly natural antioxidants, which might have supported health and longevity of people in Okinawa. Antioxidants play and important role not only in food preservation but in couteracting the risk of most cardiovascular diseases, cancer and aging. Based on our screening for antioxidant activity in Okinawan traditional edible plants, we have focused our attention on two species, such as Japanese long pepper (Piper retrofractum) and Noni (Morinda citrifolia). Both species showed strong andtioxidant activity evaluated by several methods, such as the OSI method and the DPPH radical scavenging test. From the ethyl acetate soluble fraction of P retrofractum extract, several antioxidative polyphenols including flavonoid glycosides were isolated and determined. From the leaves of M citrifolia, 8 compounds were determined including quercetin glycosides, iridoids and ferulic acid glycosides.