Visualization of water pollutant using transgenic medaka

使用转基因青鳉可视化水污染物

• 批准号: 16310041
• 负责人: KINOSHITA Masato
• 金额: $ 9.09万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16310041/
• 关键词: transgenesis; medaka; endocrine disrupter; melanin concentration hormone; Cre; loxP; コリオゲニン; 蛍光タンパク質; Tilling; トランスジェニック; LoxP; 環境; 環境ホルモン; GFP

In this study, I aimed to visualize the water pollutant in environmental water using transgenic medaka. Endocrine disrupters, especially estradiol, was used as a model chemical of pollutants.The system of visualization consists of following parts: 1) sensor promoter, 2) production of the signal in medaka body, 3) conversion of the signal, 4) amplification of signal, 5) visualization.1)As for sensor, we showed the utility of regulatory region of choriognin H (ChgH) gene. Choriogenin H is one of major egg envelope proteins and is synthesized in liver with the stimulation of estrogen and estrogen-like chemicals.2)As for signal substance, I chose melanin concentrating hormon (MCH). And I confirmed MCH was produced in medaka liver with the stimulus of estrogen.3)I found two candidate genes which were induced by MCH.4)I employed Cre-loxP system to amplify the signal. Up to now, I produced several transgenic medaka harboring loxP sequence and reporter gene. And I confirmed that cre recombinase works in medaka body.5)I showed green fluorescent protein gene and red fluorescent protein gene were useful to change the medaka body color.

在这项研究中，我的目的是可视化的水污染物在环境水体中使用转基因青鳉。本研究以内分泌干扰物雌二醇作为污染物的模型化合物，建立了一个可视化的系统，包括：1）传感器启动子，2）青鳉体内信号的产生，3）信号的转换，4）信号的放大，5）可视化。1）在传感器方面，我们展示了ChgH基因调控区的实用性。Choriogenin H是一种主要的卵膜蛋白，在雌激素和雌激素样物质的刺激下由肝脏合成。2）作为信号物质，选择了黑色素浓集激素（MCH）。3）发现了两个受MCH诱导的候选基因。4）利用Cre-loxP系统对信号进行了放大。到目前为止，我已经成功地获得了几株含有loxP序列和报告基因的转基因青鳉。5）证明了绿色荧光蛋白基因和红色荧光蛋白基因对青鳉鱼体色的改变是有效的。