Sensing device using integrated nanospaces

我们已经开发了一种新型的化学传感器的基础上的介孔薄膜（MTF），其中荧光离子载体和掩蔽剂嵌入。使用这种传感器，在水溶液中的金属离子的高选择性检测已被成功地证明。通过将前体溶液浇铸到二氧化硅微升板上的微孔底部来沉积MTF。荧光离子载体8-羟基喹啉-5-磺酸（QS）是一种典型的金属离子荧光螯合剂，通过从其水溶液中吸附引入到含有表面活性剂的介孔中。将10 μ l的15 μ M Al ^<3 +>溶液滴到微孔上，膜的荧光强度增强约10倍。QS和Al ^<3 +>之间的络合作用成功地发生在介孔附近。Al ^<3 +>的选择性检测是通过在QS存在下将1，10-菲咯啉（Phen）作为掩蔽剂引入介孔中来实现的。15 μ M的Mg ^<2 +>、Zn ^<2 +>或Cd ^<2 +>的干扰被Phen完全抑制，随后，在干扰物质存在的情况下，仅观察到Al ^<3 +>的荧光增强。此外，使用由功能化的MTF组成的传感器阵列的多个样品的同时分析首次被证明在本研究中所展示的传感原理适用于水溶液中的各种化学物种的传感。此外，在水溶液中对分析物具有差的结合能力的受体可能在含有表面活性剂的介孔中存在的疏水纳米尺寸的空腔内工作良好。这一工作不仅有助于化学装置的构建，而且有助于纳米化学的发展。

We have developed a novel chemical-sensing device based on the mesoporous thin films (MTFs) wherein both a fluoroionophore and a masking agent are embedded. Using this sensor, a highly selective detection of metal ions in an aqueous solution has been successfully demonstrated. The MTFs are deposited by casting the precursor solution onto the bottom of microwells on a silica microliter plate. The fluoroionophore 8-hydroxyquinoline-5-sulfonic acid (QS), which is a typical fluorogenic chelating agent for a variety of metal ions, is introduced into the mesopores containing surfactants by adsorption from its aqueous solution. The fluorescence intensity of the film is enhanced approximately 10 times by dropping 10 μl of 15 μM Al^<3+> solution onto the microwell. Complexation between QS and Al^<3+> occurs successfully in the vicinity of the mesopores. The selective detection of Al^<3+> is achieved by the introduction of 1,10-phenanthroline (Phen) as a masking agent into the mesopores in the presence of QS. The interferences of 15 μM of Mg^<2+>,Zn^<2+>, or Cd^<2+> are completely inhibited by Phen, and subsequently, fluorescence enhancement by only Al^<3+> is observed in the presence of the interfering species. Furthermore, simultaneous analyses of the multisamples using a sensor array composed of the functionalized MTFs are demonstrated for the first time.The sensing principle demonstrated in the present study is applicable to the sensing of a variety of chemical species in aqueous solutions. Furthermore, receptors that have poor binding capabilities to the analytes in aqueous solutions, probably work well within hydrophobic nanometer-sized cavities present in the mesopores containing surfactants. This work contributes not only to the construction of the chemical devices, but also to the development of nanochemistry.