Development of Nano-Biosensors Based on Fluorescence Enhancement by Plasmon Resonance on 3D-Nanostructure

纳米生物传感器是一种新型的由二氧化硅和金属组成的介孔网状薄膜。将众所周知的介孔二氧化硅薄膜（孔径为- 2nm）涂覆在镀有Au和Cr膜的玻璃板上。在此过程中，使用少量表面活性剂作为介孔模板，导致介孔和非介孔二氧化硅畴之间的相分离。在烧结后，Cr原子迁移到介孔二氧化硅畴中，然后将其完美地填充在mosoole中。这些cr -二氧化硅纳米复合结构域具有耐高频特性。在HF溶液蚀刻二氧化硅畴后，由Cr和二氧化硅组成的3d纳米结构作为光学透明薄膜保留在板上。与众所周知的介孔二氧化硅膜相比，该膜具有较大的介孔（40-60 nm），并且膜上的孔相互连接。我们成功地获得了介孔网络（MPN）薄膜。通过抗原抗体反应检测MPN的免疫感应能力。小鼠igg不可逆地吸附在MNP上，并在缓冲液中选择性地与FITC标记的单克隆抗体结合。荧光强度比没有MPN的平板上的类似测定高30倍。然而，没有任何证据表明等离子体共振能增强MPN的荧光。此外，利用MPN薄膜验证了酶联免疫吸附试验（ELISA）。酶在MPN中的最大反应速率比在普通96孔平板上的最大反应速率提高了50倍。

Nano-biosensors have been developed using a new type of mesoporous network thin film composed of silica and metal.The well-known mesoporous silica thin film (pore size -2 nm) was coated onto a glass plate coated with Au on Cr film. In this process, low amount of surfactants used as the template of mesopores results in phase separation between mesoporous and non-mesoporous silica domains. After sintering the plate, Cr atoms migrate into the mesoporous silica domains, and then mosopores are filled up with them perfectly. These Cr-Silica nanocomposite domains possess HF-resistance. After etching of silica domains by HF solution, a characteristic 3D-nanostructure composed of Cr and silica remains as an optically transparent thin film on the plate. The film has relatively large mesopores (40-60 nm) comparing the well-known mesoporous silica films, and the pores are mutually connected all over the film. We successfully obtain the mesoporous network (MPN) , thin film.Immuno-sensing capabilities of the MPN were examined via antigen-antibody reaction. The mouse-IgG adsorbs irreversibly into the MNP, and selectively binds with its monoclonal antibody labeled with FITC in buffer solution. The fluorescence intensity is 30 times higher than similar assay on a flat plate without the MPN. However, there are no any evidences that the fluorescence is enhanced by plasmon resonance of the MPN. Moreover, enzyme-linked immuno-sorbent assay (ELISA) was demonstrated using the MPN thin film. The maximum reaction rate of enzyme in the MPN is promoted 50 times faster than that on the flat surface of conventional 96-holes plate.