Mechanisms for all-inclusive controls of cell cycle progression, and IgM H and L-chain gene expressions by GCN5 and HDAC2

GCN5 和 HDAC2 全面控制细胞周期进程以及 IgM H 和 L 链基因表达的机制

• 批准号: 16310134
• 负责人: NAKAYAMA Tatsuo
• 金额: $ 10.05万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16310134/
• 关键词: Histone modifying enzyme; Gene targeting; DT40; Cell cycle; Immunoglobulin; Histone chaperone; Apoptosis; Gene expression

1. WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with CAF-1p48 subunit and HDAC2 i a vitro and in vivo.2. GCN5 is a supervisor in all-inclusive control of vertebrate cell cycle progression via transcription regulation of various cell cycle-related genes.3. N-terminal and C-terminal halves of HIRA exhibit different roles in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth.4. HAT1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.5. ASF1 is essential for viability and chromatin assembly during DNA replication in vertebrate cells.6. CAF-1-mediated rapid nucleosome assembly is essential for DNA replication and cell division in vertebrate cells.7. HDAC2 is necessary for controls of IgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions.

1.鸡HIRA的WD二肽基序和LXXLL基序在体内外与CAF-1 p48亚基和HDAC 2相互作用中是必需的. GCN 5是脊椎动物细胞周期进程的一个监督者，它通过转录调控多种细胞周期相关基因来全面控制细胞周期进程. HIRA的N端和C端在细胞周期相关基因的转录调控中表现出不同的作用，这些基因有助于控制脊椎动物细胞的生长. HAT 1是复制偶联染色质组装的关键，但有助于恢复脊椎动物细胞中复制阻断后产生的DNA损伤。ASF 1在脊椎动物细胞DNA复制过程中对生存力和染色质组装至关重要。CAF-1介导的核小体快速组装对于脊椎动物细胞中的DNA复制和细胞分裂是必不可少的。HDAC 2是通过EBF 1、Pax 5、Ikaros、Aiolos和E2 A基因表达控制IgM H和L链基因表达所必需的。

项目成果

Histone acetyltransferase 1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells

• DOI: 10.1016/j.bbrc.2006.05.079
• 发表时间: 2006-07-14
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Barman, Hirak Kumar;Takami, Yasunari;Nakayama, Tatsuo
• 通讯作者: Nakayama, Tatsuo

Growth inhibition and mutagenesis induced in Escherichia coli by dihydropyrazines with DNA strand-cleaving activity.

具有 DNA 链切割活性的二氢吡嗪可诱导大肠杆菌生长抑制和诱变。

• 发表时间: 2004
• 期刊: Mutation Research 560
• 作者: Takechi;S.;Yamaguchi;T.;Nomura;H.;Minematsu;T.;Nakayama;T.
• 通讯作者: T.

GCN5: a supervisor in all-inclusive control of vertebrate cell cycle progression through transcription regulation of various cell cycle-related genes

• DOI: 10.1016/j.gene.2004.12.007
• 发表时间: 2005-02-28
• 期刊: GENE
• 影响因子: 3.5
• 作者: Kikuchi, H;Takami, Y;Nakayama, T
• 通讯作者: Nakayama, T

Dicer is essential for formation of the heterochromatin structure in vertebrate cells

• DOI: 10.1038/ncb1155
• 发表时间: 2004-08-01
• 期刊: NATURE CELL BIOLOGY
• 影响因子: 21.3
• 作者: Fukagawa, T;Nogami, M;Oshimura, M
• 通讯作者: Oshimura, M

Essential Role of CAF-1-mediated Rapid Nucleosome Assembly for DNA Replication and Cell Division in Vertebrate Cells.

CAF-1 介导的快速核小体组装对脊椎动物细胞 DNA 复制和细胞分裂的重要作用。

• 发表时间: 2007
• 期刊: Molecular Biology of the Cell 18
• 作者: Kwon;M.S.;Hori;T.;Okada;M.;Fukagawa;T.;Mi-Sun Kwon;Michela Zuccolo;Yasunari Takami
• 通讯作者: Yasunari Takami