Mechanisms for all-inclusive controls of cell cycle progression, and IgM H and L-chain gene expressions by GCN5 and HDAC2

1.鸡Hira的WD二肽基序和LxxLL基序是与CAF-1p48亚基和HDAC2 I a体内外相互作用所必需的。GCN5通过转录调控多种细胞周期相关基因，全面调控脊椎动物细胞周期进程。Hira的N-末端和C-末端在调控脊椎动物细胞生长的细胞周期相关基因转录调控中发挥不同的作用。HAT1对于复制偶联染色质组装是必不可少的，但有助于恢复脊椎动物细胞复制阻断后造成的DNA损伤。在脊椎动物细胞的DNA复制过程中，ASF1对于活性和染色质组装是必不可少的。CAF-1介导的核小体快速组装是脊椎动物细胞DNA复制和细胞分裂所必需的。HDAC2是通过EBF1、Pax5、Ikaros、Aiolos和E2a基因表达调控IgM H和L链基因表达所必需的。

1. WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with CAF-1p48 subunit and HDAC2 i a vitro and in vivo.2. GCN5 is a supervisor in all-inclusive control of vertebrate cell cycle progression via transcription regulation of various cell cycle-related genes.3. N-terminal and C-terminal halves of HIRA exhibit different roles in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth.4. HAT1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.5. ASF1 is essential for viability and chromatin assembly during DNA replication in vertebrate cells.6. CAF-1-mediated rapid nucleosome assembly is essential for DNA replication and cell division in vertebrate cells.7. HDAC2 is necessary for controls of IgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions.