Mechanisms for all-inclusive controls of cell cycle progression, and IgM H and L-chain gene expressions by GCN5 and HDAC2

1.鸡HIRA的WD二肽基序和LXXLL基序在体内外与CAF-1 p48亚基和HDAC 2相互作用中是必需的. GCN 5是脊椎动物细胞周期进程的一个监督者，它通过转录调控多种细胞周期相关基因来全面控制细胞周期进程. HIRA的N端和C端在细胞周期相关基因的转录调控中表现出不同的作用，这些基因有助于控制脊椎动物细胞的生长. HAT 1是复制偶联染色质组装的关键，但有助于恢复脊椎动物细胞中复制阻断后产生的DNA损伤。ASF 1在脊椎动物细胞DNA复制过程中对生存力和染色质组装至关重要。CAF-1介导的核小体快速组装对于脊椎动物细胞中的DNA复制和细胞分裂是必不可少的。HDAC 2是通过EBF 1、Pax 5、Ikaros、Aiolos和E2 A基因表达控制IgM H和L链基因表达所必需的。

1. WD dipeptide motifs and LXXLL motif of chicken HIRA are essential for interactions with CAF-1p48 subunit and HDAC2 i a vitro and in vivo.2. GCN5 is a supervisor in all-inclusive control of vertebrate cell cycle progression via transcription regulation of various cell cycle-related genes.3. N-terminal and C-terminal halves of HIRA exhibit different roles in transcription regulation of cell cycle-related genes that contribute to control of vertebrate cell growth.4. HAT1 is dispensable for replication-coupled chromatin assembly but contributes to recover DNA damages created following replication blockage in vertebrate cells.5. ASF1 is essential for viability and chromatin assembly during DNA replication in vertebrate cells.6. CAF-1-mediated rapid nucleosome assembly is essential for DNA replication and cell division in vertebrate cells.7. HDAC2 is necessary for controls of IgM H and L-chain gene expressions via EBF1, Pax5, Ikaros, Aiolos and E2A gene expressions.