Functional analyses of chromatin structure changing-related factors in higher eukaryotic cells by gene targeting techniques

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To clarify individual roles of chromatin structure changing-related factors in higher eukaryotic cells, we generated systematically a number of chicken DT40 mutant cell lines, respectively, lacking each of appropriate genes. By analyses of these mutant cells, we obtained important results as follows. (1) CAF-1-dependent nucleosome assembly is essential in DNA replication and cell proliferation via its interaction with PCNA. (2) HDAC2 regulates indirectly gene expressions of IgM H- and ・L chains via opposite controls of gene expressions of EBF1, Pax5, Aiolos plus Ikaros, and E2A. (3) GCN5 and BCR signaling regulate cooperatively pre-mature B cell apoptosis through both increases of caspase-3 and caspase-8 activities and decreases of ICAD and IAP2.