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Core B: Transgenic and Gene-Targeting Institutional Facility

核心 B：转基因和基因靶向机构设施

基本信息

批准号：
10215556
负责人：
JAY L VIVIAN
金额：
$ 14.71万
依托单位：
UNIVERSITY OF KANSAS MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-01 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10215556
关键词：
Animal Husbandry Animal Model Animals Area Basic Science Biopsy CRISPR/Cas technology Cell Differentiation process Cell Line Cells Centers of Research Excellence Cities Clinical Research Complex Consumption Core Facility Data Detection Development Diagnostic Differentiated Gene Disease Progression Doctor of Philosophy Educational workshop Embryo Emerging Technologies Experimental Designs Faculty Gel Gene Expression Gene Targeting Gene Transfer Techniques Gene-Modified Generations Genetic Genetically Modified Animals Genotype Human Injections Integrase Kansas Lead Maintenance Medical center Methodology Methods Microinjections Modeling Modification Molecular Monitor Mus Mutation Nucleic Acids Patients Phase Pluripotent Stem Cells Process Production Rattus Reagent Regulation Research Research Personnel Research Project Grants Resource Sharing Rodent Sampling Services Site Students Technical Expertise Techniques Technology Time Training Transgenic Organisms Universities Validation Work base blastocyst cost design embryo cryopreservation embryonic stem cell experimental study functional genomics gene function genetic manipulation genome editing genomic data human disease human pluripotent stem cell in vivo in vivo Model induced pluripotent stem cell instrumentation interest lectures member model development mouse genetics mouse model new technology novel novel therapeutics operation sperm cryopreservation stem stem cell biology stem cell model tool transcription activator-like effector nucleases

项目摘要

Project Summary / Abstract The use of genetically altered mouse and stem cell models are critical tools for research members of the COBRE Project in Molecular Regulation of Cell Development and Differentiation at the University of Kansas Medical Center. The production and analysis of such models ultimately lead to better understanding of basic mechanisms of differentiation and gene expression, functional genomics of disease progression, and in vivo models for diagnostics and treatment. The Transgenic and Gene Targeting shared resource (TGIF), led by Jay L. Vivian PhD, supports members of COBRE and researchers in the Kansas City area by providing centralized technical services for the production of novel transgenic and gene-targeted rodents and genetically altered pluripotent stem cells. The Facility uses cutting edge methods, state-of-the-art instrumentation, and novel reagents for the generation of these models. The COBRE support of the TGIF allows for the development of specific initiatives in the Facility relevant to these researchers. Recent initiatives include successful integration of emerging technologies, including in vivo genome editing methods (e.g. CRISPR/Cas9 and TALE nucleases) to provide a more rapid means of generating novel mouse models. The integration of these continually evolving methods into the `toolbox' of the TGIF greatly accelerates the development of animal and stem cell models of development and differentiation, while also reducing the overall costs to COBRE researchers.

项目总结/摘要使用基因改变的小鼠和干细胞模型是COBRE研究成员的关键工具堪萨斯医科大学细胞发育和分化的分子调控项目中心这些模型的产生和分析最终会导致对基本机制的更好理解分化和基因表达，疾病进展的功能基因组学，以及诊断和治疗。转基因和基因靶向共享资源（TGIF），由杰伊L。薇薇安博士，支持COBRE成员和研究人员在堪萨斯城地区提供集中的技术为生产新型转基因和基因靶向啮齿动物以及基因改变的多能干细胞该设施使用最先进的方法，最先进的仪器和新型试剂，这些模型的生成。TGIF的COBRE支持允许制定具体举措与这些研究人员有关的设施。最近的举措包括成功整合新兴技术，包括体内基因组编辑方法（例如CRISPR/Cas9和TALE核酸酶），以提供一种新的基因组编辑方法。更快速的方法来产生新的小鼠模型。这些不断发展的方法的整合 TGIF的“工具箱”大大加速了动物和干细胞模型的发展和差异化，同时也降低了COBRE研究人员的总体成本。