Spatio-temporally resolved observation and control of neuron excitement mechanism using nonlinear laser microscopes
Spatio-temporally resolved observation and control of neuron excitement mechanism using nonlinear laser microscopes
批准号:
16360024
负责人:
MORITA Ryuji
金额:
$7.17万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (B)
财政年份:
2004
资助国家:
日本
项目状态:
已结题
起止时间:
2004 至 2006
中文摘要
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英文摘要
1.An over-one-octave spanning (460-1060 nm) ultra-broadband optical pulse was generated by self-modulation effect. It was spectrally-phase-compensated and characterized to be a 2.8 fs, 1.5 cycle-Fourier-transform-limited pulse by the modified spectral phase interferometry for direct electric-field reconstruction. It is the shortest visible and infrared pulse whose spectral phase is well determined.2.A second-harmonic laser microscope was developed for 3-dimensional spatially-resolved observation of living cells. As an example, neurons of a rat were observed. Strong signals from nuclei and axons owing to inversion-symmetry breaking were detected. Spatial resolution was less than 1.5 μm.3.A coherent anti-Stokes Raman scattering laser microscope was developed using a femtosecond laser amplifier as a light source. For onion cells, two pulses with wavelengths of 800 and 1040 nm were employed to excite the C-H vibration mode of 2900 cm-1 and the corresponding spatially-resolved signal with a wavelength of 650 nm was detected. Similarly, two pulses with wavelengths of 800 and 1100 nm were employed to excite the N-H vibration mode of 3400 cm-1 and the corresponding signal with a wavelength of 630 nm was detected. From comparison between those signals, C-H-and N-H-rich domains in cells were determined.4.Macroscopic observation of hybridization and dehybridization for methylred-intercalated DNA was performed using second-harmonic laser microscope. While hybridized one has the inversion symmetry, dehybridized one does not. Significant signals were not obtained so far because of noise. Improvement is going on by introducing other azo-dyes with higher optical nonlinearity.
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DOI:
10.1364/opex.13.002120
发表时间:
2005-03-21
期刊:
OPTICS EXPRESS
影响因子:
3.8
作者:
[Deng, YQ, Wu, ZB, Zhang, ZG]
通讯作者:
Zhang, ZG
DOI:
10.1063/1.1804238
发表时间:
2004-10
期刊:
Applied Physics Letters
影响因子:
4
作者:
[O. Takeuchi;Masahiro Aoyama;R. Oshima;Y. Okada;H. Oigawa;N. Sano;H. Shigekawa;R. Morita;M. Yamashita]
通讯作者:
O. Takeuchi;Masahiro Aoyama;R. Oshima;Y. Okada;H. Oigawa;N. Sano;H. Shigekawa;R. Morita;M. Yamashita
DOI:
--
发表时间:
2004
期刊:
Physica E 21・2-4
影响因子:
--
作者:
[M.Yamashita, et al., M.Yamashita et al., Y.Deng et al., S.Nakamura et al., M.Adachi et al., M.Yamashita et al., Y.Deng et al., S.Nakamura et al., M.Adachi et al., M.Adachi et al., S.Nakamura et al., Y.Deng et al., Toda et al.]
通讯作者:
Toda et al.
Pulse compression using direct feedback of the spectral phase from photonic crystal fiber output without the need for the Taylor expansion method
使用光子晶体光纤输出的光谱相位直接反馈进行脉冲压缩,无需泰勒展开法
DOI:
--
发表时间:
2004
期刊:
IEEE Photonics Technology Letters, 16
影响因子:
--
作者:
[Simon.Perraud, Kiyoshi Kanisawa, Zhao-Zhong Wang, Yoshiro Hirayama, M. Adachi]
通讯作者:
M. Adachi
Extended finite difference time domain analysis of induced phase modulation and four-wave mixing between two-color femtosecond laser pulses in a silica fiber with different initial delays
具有不同初始延迟的石英光纤中双色飞秒激光脉冲之间的诱导相位调制和四波混合的扩展有限差分时域分析
DOI:
--
发表时间:
2005
期刊:
Japanese Journal of Applied Physics, Part 1-Regular Papers, Brief Communications and Review 44
影响因子:
--
作者:
[Hiroaki Kotani, et al., S. Nakamura]
通讯作者:
S. Nakamura
共 14 条
Generation of intense topological light waves in the mono-cycle regime by coherent beam combining and their application
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批准号:26286056
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$10.23万
-
财政年份:2014
-
负责人:MORITA Ryuji
-
依托单位:
Novel optical physics by using ultrashort annular optical beams interacting with ring-shaped materials
-
批准号:23656040
-
项目类别:Grant-in-Aid for Challenging Exploratory Research
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资助金额:$2.41万
-
财政年份:2011
-
负责人:MORITA Ryuji
-
依托单位:
Control of spin-orbit interaction of optical pulses in the mono-cycle regime and its application
-
批准号:23360024
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$13.15万
-
财政年份:2011
-
负责人:MORITA Ryuji
-
依托单位:
Development of a single molecular switch by controlling phase and amplitude of optical electric fields in extremely short time region
-
批准号:20360025
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$11.73万
-
财政年份:2008
-
负责人:MORITA Ryuji
-
依托单位:
海外基金