Studies on autoantigens and pathogenesis in canine idiopathic encephalitis.

1)GFAP是犬坏死性脑膜脑炎和肉芽肿性脑膜脑炎中的常见自身抗原：为了鉴定犬特发性脑炎中的常见自身抗原，我们收集了40只患有坏死性脑膜脑炎（NME）或肉芽肿性脑膜脑炎（GME）的犬的脑脊液。还检查了80只患有其他CNS疾病的犬。通过对犬脑蛋白质的Western blotting和MALDI-TOF-MASS分析，确定胶质细胞酸性蛋白（GFAP）为自身抗原的候选抗原。事实上，患者的CSF与纯化的牛GFAP反应。这些结果清楚地表明GFAP是犬NME以及GME中的常见自身抗原。2）来自NME狗的CSF抑制谷氨酸摄取到正常犬星形胶质细胞中：为了阐明NME或GME对脑中谷氨酸稳态的影响，用来自NME（GME）患者的CSF处理培养的犬星形胶质细胞。在经处理的培养物中，上清液中的谷氨酸浓度升高，谷氨酸转运体（EAAT 1和2）的mRNA显著降低。因此，NME和GME抑制神经胶质谷氨酸摄取，这可导致脑中的神经元损伤。

1)GFAP is a common autoantigen in canine necrotizing nemingoencephalitis and granulomatous meningoencephalitis : To identify the common autoantigen(s) in canine idiopathic encephalitis, we collected cerebrospinal fluids from 40 dogs with necrotizing meningoencephalitis (NME) or granulomatous meningoencephalitis (GME). Eighty dogs with other CNS diseases were also examined. By Western blotting on canine brain proteins and MALDI-TOF-MASS, glial fibrillaly acidic protein (GFAP) was the candidate of autoantigen. In fact patients' CSFs reacted with purified bovine GFAP. These results clearly showed that GFAP is a common autoantigen in canine NME as well as in GME.2)CSFs from NME dogs inhibit glutamate uptake into normal canine astrocytes : To clarify the effect of NME or GME to glutamate homeostasis in the brain, cultured canine astrocytes were treated with CSFs from NME (GME) patients. Glutamate concentrations in the supernatants were elevated in the treated cultures, with the significant decrease in mRNAs of glutamate transporters (EAAT1 and 2). Thus, NME and GME inhibit glial glutamate uptake, which can lead to neuronal damage in the brain.