Prevention studies for diabetic complications based on antioxidative proteins specifically expressed in cats with Diabetes.

基于糖尿病猫中特异性表达的抗氧化蛋白的糖尿病并发症的预防研究。

• 批准号: 23658259
• 负责人: MATSUKI Naoaki
• 金额: $ 2.5万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23658259/
• 关键词: 猫; 糖尿病合併症; 抗酸化物質; ハプトグロビン; 糖尿病; 抗酸化能

Diabetes mellitus (DM) is an well described endocrine/metabolic disease. In cats, it is also well known that diabetic complications including cataract, vasculopathy and neuropathy are very mild compared to those in humans and dogs. To elucidate the reason for the mild diabetic complications in cats, endogenous antioxidative activities were examined. Blood plasma from DM cats (n=17) possessed higher hydroxyl radical-scavenging activities than those from age- and body weight-matched healthy controls (n=16). When the blood plasma from DM cats (n=3) and controls (n=3) were fractionated with dialysis columns, the hydroxyl radical-scavenging activity was found in the MW > 10kDa fraction. Differential plasma protein electrophoresis and mass spectrometry (TOF-MS) revealed haptoglobin as a candidate of hydroxyl radical-scavenger. Moreover, plasma haptoglobin concentrations and hydroxyl radical-scavenging activities showed positive correlation. Hence, haptoglobin may work as an antioxidant in DM cats, which may preventive to diabetic complications in part.

糖尿病（DM）是一种描述良好的内分泌/代谢性疾病。众所周知，与人类和狗相比，猫的糖尿病并发症包括白内障、血管病变和神经病变是非常轻微的。为了阐明猫轻度糖尿病并发症的原因，我们检测了猫的内源性抗氧化活性。糖尿病猫的血浆（n=17）比年龄和体重匹配的健康对照（n=16）具有更高的羟基自由基清除活性。用透析柱分离DM猫（n=3）和对照组（n=3）的血浆，发现MW > 10kDa部分具有清除羟基自由基的活性。差异血浆蛋白电泳和质谱分析（TOF-MS）显示，触珠蛋白是羟基自由基清除剂的候选物质。血浆触珠蛋白浓度与羟自由基清除活性呈正相关。因此，接触珠蛋白可能在糖尿病猫中起到抗氧化剂的作用，可能在一定程度上预防糖尿病并发症。