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Study of Protein Stability Synthesized in the Endoplasmic Reticulum

内质网合成蛋白稳定性研究

基本信息

批准号：
16380230
负责人：
KOIZUMI Nozomu
金额：
$ 7.49万
依托单位：
Nara Institute of Science and Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

This study aimed to clarify the mechanism to determine the stability of protein which is synthesized in the endoplasmic reticulum. Specifically major research was conducted to understand the molecular mechanism of the-ER stress response of plants using Arabidopsis. Although initial approach that is mutant screening did not work, a bZIP type transcription factor AtbZIP60 could be isolated using genomic information of Arabidopsis. AtbZIP60 is the first transcription factor that regulated the ER stress response in plants. Regulation of AtbZIP60 is very unique. That is, AtbZIP60 protein is considered to localize to the ER membrane under non-stressed condition. When plant cells perceive the ER stress, AtbZIP60 protein is cleaved by unknown mechanism. The N-terminal region of AtbZIP60 translocates to the nucleus to in order to function as a transcription factor that activates genes that are induced upon the ER stress response. Such regulation mechanism is the first finding in plant science field and is considered to be a great contribution of this study. Next step would be clarification of cleavage mechanism of AtbZIP60 protein. Since, S1P and S2P proteases do not seem to be involved in this process, alternative proteases are considered to function. According to the results of analysis of a T-DNA insertion mutant, it is clear that AtbZIP60 regulates the expression of genes induced by the ER stress, such as AtBiP3. However, it also became clear that AtbZIP60 is not an only transcription factor regulating the ER stress response. Identification of another transcription factor would be a challenging and important future study.

本研究旨在阐明内质网合成的蛋白质稳定性的决定机制。具体而言，进行了主要研究，以了解植物的ER胁迫反应的分子机制，使用拟南芥。虽然最初的方法，即突变体筛选没有工作，一个bZIP型转录因子AtbZIP 60可以分离利用拟南芥基因组信息。AtbZIP 60是植物中第一个调控内质网应激反应的转录因子。AtbZIP 60的调节非常独特。也就是说，认为AtbZIP 60蛋白在非应激条件下定位于ER膜。当植物细胞感受到ER胁迫时，AtbZIP 60蛋白被未知的机制切割。AtbZIP 60的N-末端区域易位到细胞核，以作为转录因子发挥作用，其激活在ER应激反应时诱导的基因。这种调控机制在植物科学领域尚属首次，是本研究的一大贡献。下一步将是阐明AtbZIP 60蛋白的切割机制。由于S1 P和S2 P蛋白酶似乎不参与该过程，因此认为替代蛋白酶起作用。根据T-DNA插入突变体的分析结果，很明显AtbZIP 60调节由ER应激诱导的基因的表达，例如AtBiP 3。然而，AtbZIP 60并不是调控内质网应激反应的唯一转录因子。另一个转录因子的鉴定将是一个具有挑战性和重要的未来研究。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

An Arabidopsis transcription factor, AtbZIP60, regulates the endoplasmic reticulum stress response in a manner unique to plants

DOI：
10.1073/pnas.0408941102
发表时间：
2005-04-05
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Iwata, Y;Koizumi, N
通讯作者：
Koizumi, N

Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana.