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Analysis of ferrous ion acquisition pathways in relation to cadmium tolerance and accumulation abilities of higher plants.

高等植物镉耐受性和积累能力相关的亚铁离子获取途径分析。

基本信息

批准号：
16380232
负责人：
YOSHIHARA Toshihiro
金额：
$ 10.65万
依托单位：
Central Research Institute of Electric Power Industry
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380232/
关键词：
cadminm higher plants iron acquisition pathway tolerance accumulation transporters

项目摘要

Tolerance and accumulation abilities for major divalent metals(i.e, Fe, Cu, Zn and Cd) were compared among more than 200 species/subspecies of herbal angiosperms in Japan. Then, some genes, which play a major role to transport divalent metals from the soil to roots, such as iron transporters (IRTs), were isolated from typical plants showing higher/lower cadmium tolerance and accumulation abilities, and the sequences were compared from each others. Results demonstrated that a level of correlation could be seen between the amino acid sequences at a specific unit of the molecule and the cadmium tolerance and accumulation abilities of the mother plant species. Also, physiological experiments using many rice cultivars and the statistical analyses of the results showed that root shoot divalent metal transports could play a very important role to differentiate the accumulation ability of cadmium. Furthermore, molecular/physiological experiments using tobacco indicated that, the signal regulating expression of iron-deficiency responsive genes (e.g. NtIRT1 and NtFRO2) was a mixture of long-distance- and local transmission, the signal positively regulated the gene expressions, every tissue could generate the signal and quantitatively affect the strength dependent on their level of iron deficiency. The knowledge related to metal homeostasis in plants cleared by this study would be a key to develop special plants for phytoremediation and/or contaminant-free cropping.

比较了日本200多种/亚种草本被子植物对铁、铜、锌、镉等主要二价金属的耐受性和积累能力。然后，从具有较高或较低耐镉能力和积累能力的典型植物中分离到一些在二价金属从土壤到根的运输中起主要作用的基因，如铁转运蛋白(IRTS)，并对它们的序列进行了比较。结果表明，分子中特定单元的氨基酸序列与母植物的耐镉能力和积累能力之间存在一定程度的相关性。此外，利用多个水稻品种进行的生理实验和对结果的统计分析表明，根冠二价金属运输在区分镉的积累能力方面起着非常重要的作用。此外，利用烟草进行的分子/生理实验表明，调控缺铁反应基因(如NtIRT1和NtFRO2)表达的信号是远距离和局部传递的混合体，信号正向调节基因的表达，每个组织都可以产生信号，并根据其缺铁程度定量影响信号的强度。本研究明确了植物中金属动态平衡的相关知识，这将是开发植物修复和/或无公害种植的特殊植物的关键。