Functional analysis of phosphatidyliositol-bindin proteins in fission yeast

裂殖酵母中磷脂酰肌醇结合蛋白的功能分析

• 批准号: 16380231
• 负责人: TAKEGAWA Kaoru
• 金额: $ 9.73万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16380231/
• 关键词: Fission yeast; Phosphatidylinositol; vesicle trafficking; sporulation; endocytosis; PX domain; FYVE domain; ホスファチジルイノシトール; EYVEドメイン

Recent progress in the Schizosaccharomyces pombe genome project has revealed that most of genes required for the synthesis of phosphoinositides are conserved between S.pombe and Saccharomyces cerevisiae. We have cloned the gene, vps34/pik3, encoding a phosphatidylinositol (PtdIns) 3-kinase from S.pombe. In this project, we have identified and characterized PX and FYVE domain-containing proteins in S. pombe.1.Among various PX domain-containing proteins, Vps5p and Vps17p, homologues of sorting nexins, were found to be required for efficient sporulation, vacuolar protein sorting and vacuolar biogenesis in S.pombe cells.2.The FYVE-domain containing Ste12 protein catalyzes a PtdIns 3-P 5-kinase, which is required for efficient mating. Suppressor mutants for sterility of ste12 cells were identified. Of these, sst4/vps27 has been identified. The fission yeast Sst4p contain FYVE domain, and Sst4p is shown to be required for vacuolar protein and multivesicular sortings in S.pombe.3.Valproic acid (VPA) is widely used to treat epilepsy and manic-depressive illness. A genetic screen for fission yeast mutants that show hypersensitivity to VPA was isolated. One of the genes that we identified was vps45+, which encodes a member of Sec1/Munc18 family that is implicated PtdIns-dependent membrane trafficking.

粟酒裂殖酵母基因组计划的最新进展表明，大多数合成磷酸肌醇所需的基因在粟酒裂殖酵母和酿酒酵母之间是保守的。我们已经克隆了基因，vps 34/pik 3，编码磷脂酰肌醇（PtdIns）3-激酶从粟酒裂殖酵母。在这个项目中，我们已经确定和特点的PX和FYVE结构域的蛋白质在S。在众多的PX结构域蛋白中，Vps 5 p和Vps 17 p是分选连接蛋白的同源物，它们在粟酒裂殖酵母细胞中的孢子形成、液泡蛋白分选和液泡生物合成中起重要作用。鉴定了ste 12细胞不育的抑制突变体。其中，sst 4/vps 27已被识别。裂殖酵母Sst 4p含有FYVE结构域，Sst 4p是粟酒裂殖酵母液泡蛋白和多泡分选所必需的。3.丙戊酸（Valproic acid，VPA）广泛用于治疗癫痫和躁狂抑郁症。分离出对VPA表现出超敏反应的裂殖酵母突变体的遗传筛选。我们鉴定的一个基因是vps 45+，其编码Sec 1/Munc 18家族的一个成员，该家族与PtdIns依赖的膜运输有关。

项目成果

Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe.

分选连接蛋白同源物是粟酒裂殖酵母孢子形成中磷脂酰肌醇 3-磷酸的靶标。

• 发表时间: 2004
• 期刊: Genes to Cells 9(6)
• 作者: Koga;T.;Onishi;M.;Nakamura;Y.;Nakamura;T.;Shimoda;C.et al.
• 通讯作者: C.et al.

The Sec14 family glycerophospholipid-transfer protein is required for structural integrity of the spindle pole body during meiosis in fission yeast

• DOI: 10.1111/j.1365-2443.2004.00806.x
• 发表时间: 2004-12-01
• 期刊: GENES TO CELLS
• 影响因子: 2.1
• 作者: Nakase, Y;Nakamura, T;Shimoda, C
• 通讯作者: Shimoda, C

A mitochondrial ABC transporter Atm1p is required for protection against oxidative stress and vacuolar functions in Schizosaccharomyces pombe.

粟酒裂殖酵母中需要线粒体 ABC 转运蛋白 Atm1p 来保护其免受氧化应激和液泡功能的影响。

• 发表时间: 2005
• 期刊: Biosci. Biotechnol Biochem. 69
• 作者: Yoshihara T.;et. al.;Iwaki et al.
• 通讯作者: Iwaki et al.

A role for fission yeast Rab GTPase Ypt7p in sporulation

• DOI: 10.1247/csf.30.43
• 发表时间: 2005-12-01
• 期刊: CELL STRUCTURE AND FUNCTION
• 影响因子: 1.5
• 作者: Kashiwazaki, J;Nakamura, T;Shimoda, C
• 通讯作者: Shimoda, C

Essential roles of class E Vps proteins for sorting into multivesicular bodies in Schizosaccharomyces pombe

• DOI: 10.1099/mic.0.2007/006072-0
• 发表时间: 2007-08-01
• 期刊: MICROBIOLOGY-SGM
• 影响因子: 2.8
• 作者: Iwaki, Tornoko;Onishi, Masayuki;Takegawa, Kaoru
• 通讯作者: Takegawa, Kaoru