Studies on regenerative medicine for severe diabetes mellitus by stem cell biology

干细胞生物学对重症糖尿病再生医学的研究

基本信息

  • 批准号:
    16390360
  • 负责人:
  • 金额:
    $ 7.74万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2004
  • 资助国家:
    日本
  • 起止时间:
    2004 至 2005
  • 项目状态:
    已结题

项目摘要

A new method of making bio-artificial pancreas (BAP), macro-encapsulation of islets in PET (polyethyren tetraphutarate) mesh-reinforced PVA (polyvinyl alcohol) gel by freezing and thawing, was developed. This BAP was found to keep islets viable and functional in comparison to normal free islets in vitro. Transplantation of this BAP ameliorated hyperglycemia and improved renal dysfunction and survival in mouse model of insulin-dependent diabetes mellitus. In studying subcutaneous transplantation of BAP, collagen-coated PVA bag, when applied with sustained release micro-sphere of basic FGF (fibroblast growth factor), was found to induce more angiogenesis and improved viability of included islets in comparison to non-coated PVA bag. Islet preservation studies revealed that apoptosis, a major mechanism of islet cell loss in islet transplantation tended to develop early after islet isolation and rapidly progressed during normal culture conditions. On the other hand, cold preservation in University Wisconsin solution was found to inhibit apoptosis. Pancreas regeneration studies in rats suggested that certain factors perhaps including hyperglycemia was activated by major pancreatectomy and maintained regenerative state of the remnant pancreas, since the weight of the remnant-equivalent pancreas was increased only temporally and returned to its normal weight if pancreatic duct corresponding to removed lobes in major pancreatectomy was ligated and left in the place.In stem cell studies, neuron-like cells obtained in the differentiation studies of mouse embryonic stem cells were found to improve the symptoms of Parkinson's disease in model rats. Differentiation form bone marrow-derived cells and epithelial cells of pancreatic duct to islet cells and electrical cell fusion of bone marrow cells and islet cells were also studied and interesting knowledge toward future studies was accumulated.
提出了一种新的制备生物人工胰腺(BAP)的方法,即将胰岛包埋在PET(聚乙烯四氟酸酯)网状增强聚乙烯醇(PVA)凝胶中,采用冷冻和解冻的方法制备生物人工胰腺。与正常的游离胰岛相比,这种BAP在体外可保持胰岛的活力和功能。移植该BAP可改善胰岛素依赖型糖尿病小鼠模型的高血糖,改善肾功能和生存。在BAP皮下移植研究中,胶原包被的PVA包被与碱性FGF(成纤维细胞生长因子)缓释微球一起应用时,发现与未包被的PVA包被相比,可诱导更多的血管生成并提高所含胰岛的活力。胰岛保存研究表明,细胞凋亡是胰岛移植中胰岛细胞损失的主要机制,在胰岛分离后早期发生,在正常培养条件下迅速发展。另一方面,威斯康辛大学溶液冷保存可抑制细胞凋亡。大鼠胰腺再生研究表明,胰大切除术激活了某些可能包括高血糖在内的因素,维持了残胰腺的再生状态,因为如果将胰大切除术中切除的叶对应的胰管结扎并留在原位,残胰腺的重量只是暂时增加,然后恢复到正常重量。在干细胞研究中,在小鼠胚胎干细胞分化研究中获得的神经元样细胞被发现可以改善模型大鼠帕金森病的症状。研究了骨髓源性细胞和胰管上皮细胞向胰岛细胞的分化以及骨髓细胞与胰岛细胞的电细胞融合,为今后的研究积累了有趣的知识。

项目成果

期刊论文数量(43)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Changes in pancreatic function by pancreatoduodenectomy.(in Japanese)
胰十二指肠切除术对胰腺功能的改变。(日语)
Regenerative medicine of the islet.(in Japanese), Tounyoubyou 2005
胰岛的再生医学。(日语),Tounyoubyou 2005
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Hiura A.;et al.
  • 通讯作者:
    et al.
膵島移植と人工臓器
胰岛移植和人工器官
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    永田英生;松本慎一;小野田雅彦;角 昭一郎
  • 通讯作者:
    角 昭一郎
膵・膵島移植と人工臓器
胰腺/胰岛移植和人工器官
  • DOI:
  • 发表时间:
    2005
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Matsumoto S;et al.;角 昭一郎
  • 通讯作者:
    角 昭一郎
膵島十二指腸切除前後の膵内外分泌機能.
胰岛十二指肠切除术前后的胰腺和外分泌功能。
  • DOI:
  • 发表时间:
    2004
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Noguchi H;Matsumoto S;et al.;角 昭一郎
  • 通讯作者:
    角 昭一郎
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SUMI Shoichiro其他文献

SUMI Shoichiro的其他文献

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{{ truncateString('SUMI Shoichiro', 18)}}的其他基金

Studies on subcutaneous transplantation therapy for diabetes using mesenchymal stem cells
间充质干细胞皮下移植治疗糖尿病的研究
  • 批准号:
    25461943
  • 财政年份:
    2013
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of islet regeneration therapy by stem cell biology
干细胞生物学开发胰岛再生疗法
  • 批准号:
    13854020
  • 财政年份:
    2001
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (S)
Studies on mechanism of pancreas, especially β-cell, regeneration using surgical models
利用手术模型研究胰腺尤其是β细胞的再生机制
  • 批准号:
    12671158
  • 财政年份:
    2000
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Basic studies on the mechanisms of improved glucose
改善血糖机制的基础研究
  • 批准号:
    10671189
  • 财政年份:
    1998
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

相似海外基金

Gene therapy for diabetes mellitus based on the suppression of lipotoxicity using an improved adenovirus vector
使用改进的腺病毒载体抑制脂毒性的糖尿病基因治疗
  • 批准号:
    18K14964
  • 财政年份:
    2018
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Early-Career Scientists
Gene therapy for diabetes mellitus and gene function analysis using a novel adenovirus vector
使用新型腺病毒载体进行糖尿病基因治疗和基因功能分析
  • 批准号:
    15K18939
  • 财政年份:
    2015
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Study on the differentiation and proliferation of GLP-1-producing L cells and the development of new incretin-based therapy for diabetes mellitus
产GLP-1 L细胞分化增殖研究及肠促胰岛素治疗糖尿病新疗法的开发
  • 批准号:
    20591075
  • 财政年份:
    2008
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a new approach to therapy for diabetes mellitus using leptin
使用瘦素开发治疗糖尿病的新方法
  • 批准号:
    19591048
  • 财政年份:
    2007
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of a new therapy for diabetes mellitus through Betacellulin receptor cloning
通过 Betacellulin 受体克隆开发糖尿病新疗法
  • 批准号:
    17590915
  • 财政年份:
    2005
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Analysis of Pancreatic B-Cell Development and an Animal Model of Regeneration Therapy for Diabetes Mellitus Using Transgenic Mice
转基因小鼠胰腺 B 细胞发育分析和糖尿病再生治疗动物模型
  • 批准号:
    13671194
  • 财政年份:
    2001
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Gene Therapy for diabetes mellitus with non-endocrine cells
非内分泌细胞糖尿病基因治疗
  • 批准号:
    11671137
  • 财政年份:
    1999
  • 资助金额:
    $ 7.74万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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