The Assessment of gene therapy for bone metastatic lesion of prostate cancer, using Positron Emission Tomography.

使用正电子发射断层扫描评估前列腺癌骨转移病变的基因治疗。

• 批准号: 16390464
• 负责人: GOTOH Akinobu
• 金额: $ 8.77万
• 依托单位: Hyogo College of Medicine (2005-2006)Kobe University (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390464/
• 关键词: PET; gene therapy; prostate cancer; [^<18>F]FDG; [^<18>F]FHBG; [18F]FDG

To evaluate the therapeutic effect of the gene therapy for prostate cancer, we performed Positron Emission Tomography (PET) which employed 18F-deoxyglucose ( [18F]FDG) as a tracer, and investigated accumulation of [18F]FDG in tumor. First, we inoculated PC-3 which is a human prostate cancer cell line with Balb/C nude mouse, we formed a subcutaneous or bone tumor of PC-3. After the tumor diameter was 3-5mm (4 weeks later in bone tumor), Ad-OC-TK was injected directly into the subcutaneous tumor or the bone tumor lesion. The doses used were 2.5 x 10^9 and 2.5 x 10^<10> plaque-forming units (PFU) on Day 1 and 8 and we gave VAL daily for 21 d. After 21 d, mice were anesthetized, and injected via tail vein with [18F] FDG (5.3MBq). After lh, we sacrificed mice, and evaluated accumulation of [18F] FDG in tumor. We demonstrated low accumulation of [18F] FDG in tumor treated by gene therapy compared with no treatment. This result suggested that prostate cancer cells treated by gene therapy were low glucose rate of utilization and it was capable of assessing the therapeutic effect of the gene therapy by observing accumulation of [18F] FDG. Furthermore, we similarly tested the transduction effect of HSV-TK gene by injecting 9-[(4-[18F]-fluoro-3-hydroxymethylbuty1)guanine (FHBG) via tail vein.High accumulation of [18F] FHBG was observed in tumor treated by gene therapy compared with no treatment. This result suggested the possibility of assessing the transduction effect of HSV-TK gene by observing accumulation of [18F] FHBG.

为了评价前列腺癌基因治疗的疗效，我们进行了以18F-脱氧葡萄糖([18F]FDG)为示踪剂的正电子发射断层扫描(PET)，并观察了[18F]FDG在肿瘤中的蓄积。首先，我们用Balb/C裸鼠接种人前列腺癌PC-3细胞系，形成PC-3皮下或骨肿瘤。肿瘤直径3~5 mm后(骨肿瘤4周后)，将Ad-OC-TK直接注入皮下肿瘤或骨肿瘤病灶内。第1天和第8天的剂量分别为2.5×10~(-9)和2.5×10~(-1)和2.5×10~(-1)，连续给药21d，21d后麻醉小鼠，尾静脉注射~(18)F]FDG(5.3MBq)。1h后处死小鼠，观察肿瘤内[18F]FDG的蓄积情况。我们发现，与不进行基因治疗相比，基因治疗的肿瘤中[18F]FDG的积聚较少。这一结果提示，经基因治疗的前列腺癌细胞葡萄糖利用率低，可通过观察[18F]FDG的蓄积来评价基因治疗的疗效。此外，我们同样通过尾静脉注射9-[(4-[18F]-fluoro-3-hydroxymethylbuty1)guanine(FHBG)来测试HSV-TK基因的转导效果。与未治疗的肿瘤相比，基因治疗的肿瘤组织中[18F]FHBG的积累更高。这一结果提示，通过观察[18F]FHBG的蓄积来评价HSV-TK基因的转导效果是可能的。

项目成果

Induction of immunological antitumor effects by adenovirus-mediated gene transfer of B7-1 in a murine squamous cell carcinomacell line.

通过腺病毒介导的 B7-1 基因转移在鼠鳞状细胞癌细胞系中诱导免疫抗肿瘤作用。

• 发表时间: 2007
• 期刊: Arch Otolaryngol Head Neck Surg. 第133巻・第3号
• 作者: Baba Y;Kurahashi K;Yazawa T;Saito I;Kanegae Y;Yamada Y;Nakamoto Y;Hoshitani Y.
• 通讯作者: Hoshitani Y.

Clinical value of manual fusion of PET and CT images in patients with suspected recurrent colorectal cancer

• DOI: 10.2214/ajr.05.0708
• 发表时间: 2007-01-01
• 期刊: AMERICAN JOURNAL OF ROENTGENOLOGY
• 影响因子: 5
• 作者: Nakamoto, Yuji;Sakamoto, Setsu;Togashi, Kaori
• 通讯作者: Togashi, Kaori

泌尿器科癌に対する遺伝子治療

泌尿系癌症的基因治疗

• 发表时间: 2006
• 期刊: 臨床泌尿器科 第60巻・第5号
• 作者: Hamada K.;et al.;Yushi Nakamoto;Hinata N.;Matsumoto K.;白川利朗;Matsumoto K.;後藤章暢
• 通讯作者: 後藤章暢

二次元および三次元PET収集における雑音等計数と再構成画像の画質の評価

2D 和 3D PET 采集中的噪声计数以及重建图像的图像质量评估

• 发表时间: 2006
• 期刊: 日本放射線技術学会雑誌 第62巻・第8号
• 作者: Hinata N.;et al.;Matsumoto K.;Matsumoto K.;Katsuyuki Hamada;Nobuyuki Hinata;松本圭一
• 通讯作者: 松本圭一

Accuracy of attenuation coefficient obtained by 137Cs single-transmission scanning in PET : comparison with conventional germanium line source.

PET 中 137Cs 单透射扫描获得的衰减系数的准确性：与传统锗线源的比较。

• 发表时间: 2006
• 期刊: Nippon Hoshasen Gijutsu Gakkai Zasshi 第62巻・第2号
• 作者: Hamada K.;et al.;Yushi Nakamoto;Hinata N.;Matsumoto K.;白川利朗;Matsumoto K.
• 通讯作者: Matsumoto K.