Fabrication of the high osteoconductive bone replacement

高骨传导性骨替代物的制造

• 批准号: 16390559
• 负责人: ISHIKAWA Kunio
• 金额: $ 9.02万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390559/
• 关键词: biomaterial; carbonate apatite; nano material; apatite; osteoclasts; osteoblasts; osteoconductivity; bone replacement; 骨; 骨補填材; 組織工学; ケイ素

The objective of this research is to fabricate high osteoconductive bone replacement with carbonate apatite composition since composition of the epiphysis of growing bone is carbonate apatite containing larger amount of carbonate ions. For the achievement of this objective two requirements should be solved. One is the high osteoconducitivity similar to hydroxyapatite and the other is the replacement to bone similar to the autograft.In this year, feasibility of the bone replacement with carbonate apatite in its composition was evaluated using osteoblasts, osteoclasts and experimental animal. The carbonate apatite block was prepared using calcite block as precursor.Osteoblast-like cell was prepared by differentiating bone marrow cell was cultured on the carbonate apatite disk using sintered hydroxyapaite as control. It was found that carbonate apatite showed almost the same initial cell attachment, proliferation and differentiation. In the case of evaluation using osteoclasts, no resorption pits were observed on the surface of sintered hydroxyapatite which was used as control. However, resporption pits were observed on the surface of carbonate apatite disk indicating that carbonate apatite could be a bone replacement.As the next step, bone defect made at the experimental animal was reconstructed with carbonate apatite granule or sintered hydroxyapatite granular for the histological evaluation. Both carbonate apatite granular and sintered hydroxyapatite granular showed good osteocondutivity and thus covered with the new bone. In the case of carbonate apatite, osteoclastic resporption was also found.It. was concluded, therefore that carbonate apatite could be an ideal bone replacement based on the result obtained in the present study.

这项研究的目的是用碳酸盐磷灰石组成来制造高骨导导的骨替代，因为生长骨的骨epiphysis的组成是含有较大量的碳酸盐离子的碳酸盐磷灰石。为了实现这一目标，应解决两个要求。一种是与羟基磷灰石相似的高骨施用率，另一种是替换为骨骼类似于自体移植。在今年，使用成骨细胞，成骨细胞和实验动物评估了使用成骨细胞，成骨细胞，用碳酸盐磷灰石替换碳酸盐磷灰石的可行性。使用方解石块作为前体制备了碳酸盐磷灰石块。通过区分骨髓细胞以对照为对照在碳酸盐磷灰石盘上培养骨细胞样细胞。发现碳酸盐磷灰石显示出几乎相同的初始细胞附着，增殖和分化。在使用破骨细胞评估的情况下，在被用作对照的烧结的羟基磷灰石表面上未观察到吸收坑。然而，在碳酸盐磷灰石盘的表面上观察到吸收凹坑，表明碳酸盐磷灰石可能是骨骼的替代。碳酸盐磷灰石颗粒状和烧结的羟基磷灰石颗粒都表现出良好的骨化性，因此被新骨覆盖。在碳酸盐磷灰石的情况下，还发现了破骨碎屑的呼吸。得出结论，因此，根据本研究中获得的结果，碳酸盐磷灰石可能是理想的骨骼替代品。

项目成果

Effect of molding pressure on fabrication of low-crystalline calcite block

• DOI: 10.1007/s10856-006-0028-7
• 发表时间: 2008-02-01
• 期刊: JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
• 影响因子: 3.7
• 作者: Lin, Xin;Matsuya, Shigeki;Ishikawa, Kunio
• 通讯作者: Ishikawa, Kunio

Hardening of calcium hydroxide compact by carbonization.

通过碳化使氢氧化钙压块硬化。

• 发表时间: 2004
• 期刊: Journal of the Ceramic Society of Japan 112
• 作者: Yumiko Suzuki;Shigeki Matsuya;Koh-ichi Udoh;Masaharu Nakagawa;Yoshihiro Tsukiyama;Kiyoshi Koyano;Kunio Ishikawa;K.Ishikawa et al.
• 通讯作者: K.Ishikawa et al.

Development of macropores in calcium carbonate body using novel carbonation method of calcium hydroxide/sodium chloride composite

• DOI: 10.1007/s10853-006-0747-7
• 发表时间: 2007-04
• 期刊: Journal of Materials Science
• 影响因子: 4.5
• 作者: Yoong Lee;Y. Hahm;S. Matsuya;M. Nakagawa;K. Ishikawa

Characterization of macroporous carbonate-substituted hydroxyapatite bodies prepared in different phosphate solutions

• DOI: 10.1007/s10853-007-1629-3
• 发表时间: 2007-09-01
• 期刊: JOURNAL OF MATERIALS SCIENCE
• 影响因子: 4.5
• 作者: Lee, Yoong;Hahm, Yeong Min;Ishikawa, Kunio
• 通讯作者: Ishikawa, Kunio

Fablication of macroporous carbonate apatite foam by hydrothermal conversion of a-tricalcium phosphate in carbonate solutions.

通过碳酸盐溶液中α-磷酸三钙的水热转化制备大孔碳酸盐磷灰石泡沫。

• 期刊: J Biomed Mater Res A (in press)
• 作者: Wakae H;Takeuchi A;Udoh K;Matsuya S;Munar M;LeGeros RZ;Nakagawa M;Nakasima A;Ishikawa K
• 通讯作者: Ishikawa K