ポリフェノール類の組み合わせによる活性酸素除去能の増幅に関する研究

多酚组合增强活性氧去除能力的研究

• 批准号: 16780091
• 负责人: 中村 浩蔵
• 金额: $ 1.6万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16780091/
• 关键词: ポリフェノール; SOD活性; 混合物; HPLC分析; 増幅効果; フェノール性物質

本年度は,まず,昨年度得られた研究結果の検証を行った。複数の実験者で実施されたin vitro(WST法)での試験結果を総合すると,昨年度得られた組み合わせによる増幅効果は実験誤差であり,今回用いた米中に含まれるポリフェノール類の組み合わせによる活性酸素除去能の増幅効果はin vitroでは見られなかった。この結果は,これまでの研究成果(文献1,2)を支持し,複数のポリフェノール類による総活性酸素除去能は,各化合物が持つ活性酸素除去能の和とみなせた。また,米抽出物に見られた構成化合物の活性酸素除去能の総和を大きく超える活性は,他の抗酸化物質との増幅作用であると推測された。昨年度,生体内におけるポリフェノール類の作用を検証するため,ポリフェノール類分析方法の開発を行ったが,本年度は,配糖体,プロシアニジン類を含めたポリフェノール類分析方法の開発を行い,適応範囲を拡大した。そして,ポリフェノール類(P-クマル酸,クロロゲン酸,エピカテキン)を単体および2種類を組み合わせて実験動物(ラット)に経口投与し,血漿中の化合物の検出と定量を行い,活性酸素除去能をWST法で測定した。その結果,全ての化合物が投与1時間後の血漿中から検出され,定量の結果,約7〜17%吸収されていることがわかった。血漿活性酸素除去能測定の結果,P-クマル酸では有意な活性上昇はみられなかったものの,クロロゲン酸,エピカテキン,組み合わせ投与では有意に活性が上昇し,組み合わせによる活性増幅の傾向は見られたが有意な差は見られなかった。文献1;Food Chemistry,61(1・2),pp71-75,1998,文献2;FEES Letter,427,pp305-308,1998

This year, the results of the study were verified. The results of the test were combined in vitro(WST method), and the results of the test were combined in vitro last year. The results of the test were combined in vitro last year. The results of the test were combined in vitro last year. The results of the test were combined in vitro last year. The results of the test were combined in vitro last year. These results are supported by the previous research results (Ref. 1, 2), which show that the active acid removal activities of various compounds are consistent with each other. In addition, rice extract can be seen as a component of the active acid removal activity of the compound, and its anti-acid substances can be predicted to increase the amplitude of the activity. Last year, the role of polysaccharides in vivo was verified, and the development of polysaccharides analytical methods was carried out. This year, the development of polysaccharides analytical methods, including polysaccharides, has expanded. In addition, the active acid removal energy was determined by WST method when the compound was administered to animals in the oral cavity in combination with two kinds of monomer groups. As a result, the total amount of the compound was detected in the plasma 1 time after administration, and the quantitative results showed that about 7 ~ 17% of the compound was absorbed. As a result of the determination of plasma active acid removal energy, the activity of P-acid was increased intentionally, and the activity of P-acid was increased intentionally. Literature 1;Food Chemistry,61(1·2),pp71- 75, 1998, Literature 2;FEES Letter,427,pp305- 308, 1998

项目成果

High-performance liquid chromatographic determination of phenolic compounds in rice.

• DOI: 10.1016/j.chroma.2004.11.075
• 发表时间: 2005-01
• 期刊: Journal of chromatography. A
• 作者: Su Tian;K. Nakamura;T. Cui;H. Kayahara