Applications of molecular nanotechnology for human disease using DNA-encode based gene expression analysis

使用基于 DNA 编码的基因表达分析的分子纳米技术在人类疾病中的应用

• 批准号: 17300095
• 负责人: OHYASHIKI Junko
• 金额: $ 5.18万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300095/
• 关键词: DNA computer; gene expression analysis; absolute quantification; viral infection; logical operation; DNAマイクロアレイ

Despite the fact that DNA microarrays can simultaneously monitor the expression of thousands of genes, it is difficult to compare microarray results of different platforms in various organisms (i.e. humans and pathogens). We established an application for gene expression analysis of human infectious disease which enables to analyze gene expression in both host and pathogen on a single platform. In our system, the target-specific sequences were concerted into the DNA code number, i.e., DNA encoding-based analysis (DEAN) which permits reproducible hybridization, regardless of organisms, on a single platform. The wet experiments were configured as robotic benchtop workstation We applied the method in a viral infection model, and demonstrated the possibility that the DEAN system is in principle a DNA-based computer which has applications for understanding the complex human immunoregulatory system in response to pathogens. This method is not application of quantitative gene expression analysis. Instead, it will provide insights into positive impact on research of biocomputing in living system and human diseases.

尽管DNA微阵列可以同时监测数千个基因的表达，但很难比较不同生物体（即人类和病原体）中不同平台的微阵列结果。我们建立了一个用于人类感染性疾病基因表达分析的应用程序，该应用程序能够在单个平台上分析宿主和病原体中的基因表达。在我们的系统中，靶特异性序列被协调成DNA代码编号，即，基于DNA编码的分析（DEAN），允许在单一平台上进行可重复的杂交，而不管生物体如何。湿实验被配置为机器人台式工作站。我们将该方法应用于病毒感染模型，并证明了DEAN系统原则上是基于DNA的计算机的可能性，其具有用于理解响应于病原体的复杂的人类免疫调节系统的应用。该方法不是定量基因表达分析的应用。相反，它将为生物计算在生命系统和人类疾病研究中的积极影响提供见解。

项目成果

Telomerase inhibition with a novel G-quadruplex-interactive agent, telomestatin:: in vitro and in vivo studies in acute leukemia

• DOI: 10.1038/sj.onc.1209577
• 发表时间: 2006-09-21
• 期刊: ONCOGENE
• 影响因子: 8
• 作者: Tauchi, T.;Shin-ya, K.;Ohyashiki, K.
• 通讯作者: Ohyashiki, K.

Transcriptional profiling of Epstein-Barr virus (EBV) genes and host cellular genes in nasal NK/T-cell lymphoma and chronic active EBV infection.

• DOI: 10.1038/sj.bjc.6602968
• 发表时间: 2006-02-27
• 期刊: British journal of cancer
• 影响因子: 8.8

DNAコンピューターによる演算を応用した遺伝子発現解析:診断デバイスとしての意義

使用 DNA 计算机计算进行基因表达分析：作为诊断设备的意义

• 发表时间: 2006
• 作者: Takaku;T.;Ohyashiki;JH.;Zhang;Y.;Ohyashiki;K;Tomonari Sumi and Hideo Sekino;Takaku T. et al.;Tomonari Sumi and Hideo Sekino;Ohyashiki JH;大屋敷 純子
• 通讯作者: 大屋敷 純子

Induction of heme oxygenase-1 by cobalt protoporphyrin enhances the antitumour effect of bortezomib in adult T-cell leukaemia cells

• DOI: 10.1038/sj.bjc.6604003
• 发表时间: 2007-10-16
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Hamamura, R. S.;Ohyashiki, J. H.;Ohyashiki, K.
• 通讯作者: Ohyashiki, K.

Gene expression analysis using DNA computing

使用 DNA 计算进行基因表达分析

• 发表时间: 2006
• 作者: Ohvashiki;JH
• 通讯作者: JH