Production of high sensitive blood test systems using carbon nanotube biosensor

使用碳纳米管生物传感器生产高灵敏度血液检测系统

• 批准号: 17300167
• 负责人: ISHII Atsushi
• 金额: $ 4.8万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300167/
• 关键词: Infectious disease; Virus; Nanotubes, Fullerene; Electronics device, Instruments; Crisis control

In this project, it has been aimed at the development of the device which can quickly detect the molecular interacton with high sensitivity, by combining the electric properties of carbon nanotubes, immobilization technology of the solid surface, and biotechnology. As an application of it, we tried to build the high sensitive test systems for human blood, especially the detection system of pathogenic viruses.(1) Fabrication of CNT-FET by chemical vapor deposition (CVD) method. In order to improve the process yield of the CNT biosensor and to uniform the electric property of the sensor, we optimized the fabrication process of the CNT biosensor. (i) Controlling the density of CNTs by using variety of metal multilayer films as catalysts, (ii) Controlling the density of CNTs with thermal CVD condition, (iii) Controlling a number of bridging CNTs by optimization of the catalyst dimension, (iv) Installation of the load lock chamber for EB deposition system, (v) Improvement in the lift-off pr … More ocess of the electrode patterning, (vi) Introduction of the automatic measurement system for the CNT biosensor, (vii) Selective breakdown of metallic CNTs. By these optimized processes, the yield ratio of conducting sensor and FET sensor improved 12.5 times and 5.2 times respectively.(2) Fabrication of CNT-FET by CNT immobilization through self-assedmbled membrane A nanotube FET device was fabricated on a cover glass after fabricationg a sol-gel layer of 3-aminopropyltriethoxysilane (APS) at the planned site of source and drain electrodes on the glass substrate, and after subsequent immobilizing SWCNT dispersion, which is treated using a mixture of acids and hydroxyperoxide. About 60% of the devices made by the above method showed a FET property. This devices, which can reproducibly detect pH, is applicable for use as a immunosensor through modification of the CNT surface, although it showed lower sensitivity compared to that fabricated by the CVD method.(3) Detection of Hepatitis B virus surface antigen (HBs antigen) To prepare the CNT-biosensor for detection of Hepatitis B virus surface antigen, anti-HBs antibodiy was immobilized on the reverse side of CNT put on the one side of silicon wafer. HBs surface antigen was reproducibly able to measure ranging from 10 fg/ ml to 100 pg/ml. Additon of human serum at final concentration of 5% to analyte had no effect to the measurement of the CNT-biosensor in respect to its sensitivity and accuracy. Less

本计画结合奈米碳管的电性、固体表面固定化技术及生物技术，致力于开发可快速且高灵敏度侦测分子间相互作用的装置。作为该技术的应用，我们尝试建立高灵敏度的人体血液检测系统，特别是致病性病毒的检测系统。(1)用化学气相沉积（CVD）方法制备CNT-FET。为了提高碳纳米管生物传感器的工艺良率和均匀的传感器的电性能，我们优化了碳纳米管生物传感器的制备工艺。(i)通过使用多种金属多层膜作为催化剂来控制碳纳米管的密度，（ii）通过热CVD条件来控制碳纳米管的密度，（iii）通过优化催化剂尺寸来控制桥接碳纳米管的数量，（iv）用于EB沉积系统的负载锁定室的安装，（v）提高剥离压力。 ...更多信息 电极图案化的过程，（vi）CNT生物传感器的自动测量系统的引入，（vii）金属CNT的选择性击穿。通过优化工艺，电导传感器和场效应管传感器的成品率分别提高了12.5倍和5.2倍。(2)在玻璃衬底上的源极和漏极的计划位置处制造3-氨丙基三乙氧基硅烷（APS）的溶胶-凝胶层之后，并且在随后固定SWCNT分散体之后，在盖玻片上制造纳米管FET器件，所述SWCNT分散体使用酸和羟基过氧化物的混合物处理。通过上述方法制造的器件中约60%显示出FET特性。该装置，它可以重复检测pH值，是适用于作为免疫传感器通过修饰的CNT表面，虽然它显示出较低的灵敏度相比，由CVD方法制造。(3)B型肝炎病毒表面抗原（HBs抗原）的检测为了制备用于检测B型肝炎病毒表面抗原的CNT-生物传感器，将抗-HBs抗体固定在置于硅片一侧的CNT的背面。HBs表面抗原可重复测量，范围为10 fg/ ml至100 pg/ml。添加终浓度为5%的人血清对CNT生物传感器的灵敏度和准确度没有影响。少

项目成果

カーボンナノチューブ電界効果トランジスタの製造方法

碳纳米管场效应晶体管的制造方法

• 发表时间: 2007

電界効果トランジスタ、そねを具備するバイオセンサ、および検出方法

场效应晶体管、具有肋的生物传感器及检测方法

• 发表时间: 2006

電界効果トランジスタ、それを具備するバイオセンサ、および検出方法

场效应晶体管、包括该场效应晶体管的生物传感器以及检测方法

• 发表时间: 2006

「カーボンナノチューブセンサの作製方法」

《碳纳米管传感器的制造方法》

• 发表时间: 2005

Application of crabon nanotubes for detecting anti-hemagglutinins based on antigen-antibody interatction

基于抗原抗体相互作用的crabon纳米管在检测抗血凝素中的应用

• 发表时间: 2005
• 期刊: Biosens. Bioelectron. 21
• 作者: S. Takeda;A. Sbagyo;Y. Sakoda;A. Ishii;M. Sawamura;K. Sueoka;H. Kida;K. Mukasa;K. Matsumoto
• 通讯作者: K. Matsumoto