Development of bio-dosimetry for the evaluation of low dose radiation and carcinogenic risk estimation

开发用于低剂量辐射评估和致癌风险评估的生物剂量测定法

基本信息

  • 批准号:
    17310036
  • 负责人:
  • 金额:
    $ 10.86万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2005
  • 资助国家:
    日本
  • 起止时间:
    2005 至 2007
  • 项目状态:
    已结题

项目摘要

We have tried to develop the hypersensitive mouse model and molecular bio-dosimery using DNA damage response proteins based on these functional analyses for the estimation of carcinogenic risk and dose after radiation exposure.1. Development of hypersensitive monitor mouse for the estimation of low-dose radiation exposure and analysis of its biological characterThe Y-family DNA polymerases play roles for maintenance of genetic stability. Malfunction of the polymerases affect cancer susceptibility. Revl, the number of the Y-family DNA polymerase, has deoxycytidyl transferase activity and plays the central role on the translesion DNA synthesis known as error-prone DNA repair. In order to develop hypersensitive model mouse for radiation exposure, we have generated Levi transgenic mice (Rev1 mouse). Therefore Levi mouse showed increased susceptibility to tumorigenesis after carcinogenic insult, we analyzed the biological character of this mouse. Furthermore, to determine how increasing .RE … More V1 affects the biological phenotype of cells, human fibrosarcoma cells were transfected with hREV1 expression plasmid having tetracycline regulation system and then the stable sublines were isolated. We found that the cells expressing hREV1 were resistant to the cytotoxic effect of X-ray irradiation more than parental cells. This observation suggests the survived cells may increase the accumulation of mutations after the repair process of damaged DNA.2. Research on the development of molecular bio-dosimery for low dose radiationIn order to develop new molecular bio-dosimery, we have focused our research on functional analysis of protein complex on damaged site of genome including Histone H2AX and REV1 complex which are visualized as foci and countable in cell nucleus. We have detected the possible candidate molecules for the molecular bio-dosimery. We found DNA damage-dependent modification of H2AX enhances chromatin dynamics. We also succeeded in analysis of translesion DNA sythesis on the reconstituteion system. Less
本研究尝试建立小鼠过敏模型,并利用DNA损伤反应蛋白进行分子生物剂量学研究,以评估辐射后致癌风险和剂量.低剂量辐射敏感性监测小鼠的研制及其生物学特性分析Y家族DNA聚合酶在维持遗传稳定性中起重要作用。聚合酶的功能障碍影响癌症易感性。Revl是Y家族DNA聚合酶的编号,具有脱氧胞苷酰转移酶活性,在跨损伤DNA合成中起核心作用,称为易错DNA修复。为了建立辐射致敏小鼠模型,我们建立了Levi转基因小鼠(Rev1小鼠)。因此,Levi小鼠在受到致癌物损伤后,表现出对肿瘤发生的易感性增加,我们对该小鼠的生物学特性进行了分析。此外,为了确定如何增加.RE ...更多信息 V1影响细胞的生物学表型,将具有四环素调控系统的hREV 1表达质粒转染人纤维肉瘤细胞,获得稳定的细胞亚系。我们发现,表达hREV 1的细胞对X射线照射的细胞毒性作用的抵抗力比亲本细胞更强。这一观察结果表明,在受损DNA的修复过程后,存活的细胞可能会增加突变的积累。低剂量辐射分子生物剂量学的发展研究为了发展新的分子生物剂量学,我们重点研究了基因组损伤位点蛋白复合物的功能分析,包括细胞核中可见的、可计数的组蛋白H2AX和REV 1复合物。我们已经发现了可能的候选分子的分子生物剂量学。我们发现H2AX的DNA损伤依赖性修饰增强了染色质动力学。我们还成功地分析了在重组系统上的跨损伤DNA合成。少

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Dynamics of human replication factors in the elongation phase of DNA replication.
  • DOI:
    10.1093/nar/gkm822
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    14.9
  • 作者:
    Masuda Y;Suzuki M;Piao J;Gu Y;Tsurimoto T;Kamiya K
  • 通讯作者:
    Kamiya K
損傷乗り越えDNA合成と発癌
克服损伤和致癌作用的 DNA 合成
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Watanabe;H.;増田雄司
  • 通讯作者:
    増田雄司
Translesion DNA synthesis and cancer
跨损伤 DNA 合成与癌症
  • DOI:
  • 发表时间:
    2007
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Masuda;Y
  • 通讯作者:
    Y
Mechanism of radiation carcinogenesis
放射性致癌机制
小腸線窩再生と生存率を用いたマウスにおける発酵乳の放射線防御作用
发酵乳对小鼠小肠窝再生的辐射防护作用和存活率
  • DOI:
  • 发表时间:
    2006
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Masuda;Y.;明神有紀
  • 通讯作者:
    明神有紀
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KAMIYA Kenji其他文献

An experimental approach for analysis of biological effect of low dose radiation and factors affecting DSB repair fidelity
低剂量辐射生物学效应及DSB修复保真度影响因素分析的实验方法
  • DOI:
  • 发表时间:
    2015
  • 期刊:
  • 影响因子:
    0
  • 作者:
    CAO Lili;KAWAI Hidehiko;SASATANI Megumi;IIZUKA Daisuke;MASUDA Yuji;INABA Toshiya;SUZUKI Keiji;OOTSUYAMA Akira;UMATA Toshiyuki;KAMIYA Kenji;SUZUKI Fumio;Hiroshi Tauchi
  • 通讯作者:
    Hiroshi Tauchi
The boundary between 'bad' and 'good' outsiders and the construction of unifying elements underpinning rural communities.
“坏”和“好”外来者之间的界限以及支撑农村社区的统一元素的建设。
  • DOI:
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    0
  • 作者:
    CAO Lili;KAWAI Hidehiko;SASATANI Megumi;IIZUKA Daisuke;MASUDA Yuji;INABA Toshiya;SUZUKI Keiji;OOTSUYAMA Akira;UMATA Toshiyuki;KAMIYA Kenji;SUZUKI Fumio;加賀爪優;Shiro Horiuchi
  • 通讯作者:
    Shiro Horiuchi
耳間時間差が音像の分離知覚に与える影響
耳间时间差对声像分离知觉的影响
  • DOI:
  • 发表时间:
    2014
  • 期刊:
  • 影响因子:
    0
  • 作者:
    MASUDA Yuji;SUZUKI Miki;KAWAI Hidehiko;HISHIKI Asami;HASHIMOTO Hiroshi;MASUTANI Chikahide;HISHIDA Takashi;SUZUKI Fumio;KAMIYA Kenji;近藤成一;森川大輔
  • 通讯作者:
    森川大輔

KAMIYA Kenji的其他文献

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{{ truncateString('KAMIYA Kenji', 18)}}的其他基金

Development of bio-dosimetry methods using radiation responsive urinary biomarker
使用辐射响应尿生物标志物开发生物剂量测定方法
  • 批准号:
    25550031
  • 财政年份:
    2013
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Development of a biological dosimeter to detect the DNA damage induced by low dose radiation and carcinogenic risk evaluation
开发生物剂量计检测低剂量辐射引起的DNA损伤及致癌风险评估
  • 批准号:
    22310037
  • 财政年份:
    2010
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of bio-dosimetry for the evaluation of low dose radiation and carcinogenic risk estimation
开发用于低剂量辐射评估和致癌风险评估的生物剂量测定法
  • 批准号:
    14380252
  • 财政年份:
    2002
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of molecular bio-dosimeiry and monitor mice for the detection of radiation dose exposed by tritium water
开发用于检测氚水辐射剂量的分子生物剂量学和监测小鼠
  • 批准号:
    12558049
  • 财政年份:
    2000
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analysis of molecular mechanism of radiation-induced cancer and risk estimation
辐射诱发癌症的分子机制分析及风险评估
  • 批准号:
    11680549
  • 财政年份:
    1999
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Genetic analysis for cancer risk estimation of low-dose radiation exposure.
低剂量辐射暴露癌症风险评估的遗传分析。
  • 批准号:
    09480123
  • 财政年份:
    1997
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Purification and Characterization of New Mammary Cell Growth Factor from Rat Pituitary Tumors : Preliminary Report.
大鼠垂体肿瘤中新型乳腺细胞生长因子的纯化和表征:初步报告。
  • 批准号:
    01570193
  • 财政年份:
    1989
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)

相似海外基金

Natural history of genome damage
基因组损伤的自然史
  • 批准号:
    21H04927
  • 财政年份:
    2021
  • 资助金额:
    $ 10.86万
  • 项目类别:
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Defining the altered FUS-PARP-1-DNA Ligase III axis and its implications to nuclear and mitochondrial genome damage response in Amyotrophic Lateral Sclerosis (ALS) and Frontotemporal Dementia (FTD)
定义改变的 FUS-PARP-1-DNA 连接酶 III 轴及其对肌萎缩侧索硬化症 (ALS) 和额颞叶痴呆 (FTD) 中核和线粒体基因组损伤反应的影响
  • 批准号:
    9980670
  • 财政年份:
    2020
  • 资助金额:
    $ 10.86万
  • 项目类别:
Molecular mechanisms underlying replicative stress and genome damage in pluripotent stem cells
多能干细胞复制应激和基因组损伤的分子机制
  • 批准号:
    2283666
  • 财政年份:
    2019
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Studentship
Repair of Oxidative Genome Damage Associated with Gene Activation
修复与基因激活相关的氧化基因组损伤
  • 批准号:
    8639248
  • 财政年份:
    2014
  • 资助金额:
    $ 10.86万
  • 项目类别:
Genome Damage and Stability Centre - Studentship
基因组损伤与稳定中心 - 学生奖学金
  • 批准号:
    MR/N50189X/1
  • 财政年份:
    2014
  • 资助金额:
    $ 10.86万
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    Training Grant
Repair of Oxidative Genome Damage Associated with Gene Activation
修复与基因激活相关的氧化基因组损伤
  • 批准号:
    8837028
  • 财政年份:
    2014
  • 资助金额:
    $ 10.86万
  • 项目类别:
Development of deep-sequencing approach for genome damage repair
开发基因组损伤修复深度测序方法
  • 批准号:
    26640117
  • 财政年份:
    2014
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Repair of Oxidative Genome Damage Associated with Gene Activation
修复与基因激活相关的氧化基因组损伤
  • 批准号:
    9207767
  • 财政年份:
    2014
  • 资助金额:
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Mechanism of Genome Damage Response Induced by Ultrasoun
超声诱导基因组损伤反应机制
  • 批准号:
    23650304
  • 财政年份:
    2011
  • 资助金额:
    $ 10.86万
  • 项目类别:
    Grant-in-Aid for Challenging Exploratory Research
Nutritional and genetic factors associated with genome damage in children
与儿童基因组损伤相关的营养和遗传因素
  • 批准号:
    nhmrc : 572623
  • 财政年份:
    2009
  • 资助金额:
    $ 10.86万
  • 项目类别:
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