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Nanotransport device by integrating MEMS and biomolecular motors

集成MEMS和生物分子马达的纳米传输装置

基本信息

批准号：
17310082
负责人：
COLLARD Dominique
金额：
$ 9.6万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17310082/
关键词：
Nanotechnology Microfluidic Motor proteins Molecular transportation Molecular sorting Kinesin Dynein Microtubule MEMS Micro TAS モータタンパク

项目摘要

The project concerns nano transportation systems by fusion of MEMS and biomolecular motors. The goal was reached by demonstrating a device that permits the sorting and the transportation of beads by motor proteins. The motor proteins are kinesin moving along microtubules.The microtubules need to be oriented before their immobilization in the device. The orientation is performed by gliding assay under fluid microflow. Dynein molecules are immobilized on a microchannel glass surface and microtubules were transported by dynein motion. Moving directions of gliding microtubules were controlled by a pressure-driven flow through the microchannel, and their polarities were oriented in the designated direction. The polarities were checked by the unidirectional transport of kinesin-coated microspheres. Successful orientation yield were measured between 80% and 91%.The integration of fluid flow for the MT orientation was investigated by 2 approaches :An AC electro-osmotic pump was integrated to s … More teer and align microtubules on a chip. An adapted design, based on asymmetric interdigitated electrodes was develop to deal with high conductive buffer. To visualize the pumping efficiency, transparent planar microelectrode process was developed using Indium-Tin Oxide (ITO) deposition. Electrostatic parallel-plate actuators to steer the fluid motion were also developed. An efficient and stable actuation operation was been successfully achieved.To investigate the feasibility of microtubule self-assembly, the adsorption of microtubules was studied during the drying process of liquid droplets on various substrates.Finally, a bio-hybrid microsystem for sorting and carrying target molecules by bio-functional molecules have been realized. Different target molecules, biotin-4-fluorescein and Rabbit anti-mouse IgG, were selectively attached on different beads (Streptavidin-coated and protein A-coated beads). Target molecules were separately transported by the motor protein system (kinesin on beads / microtubules on chip) without any liquid manipulation proving the feasibility of multiple target molecules on multiple beads sorter. Less

该项目涉及通过MEMS和生物分子发动机融合的纳米运输系统。这个目标是通过展示一种允许马达蛋白对珠子进行分选和运输的装置来实现的。马达蛋白是沿着微管运动的驱动蛋白，微管在固定在装置中之前需要定向。通过流体微流下的滑动测定进行取向。将动力蛋白分子固定在微通道玻璃表面上，并通过动力蛋白运动来运输微管。通过微通道的压力驱动流动控制滑动微管的移动方向，并且它们的极性在指定方向上取向。通过驱动蛋白包被的微球的单向运输检查极性。成功的取向率在80%和91%之间。通过两种方法研究了MT取向的流体流动的集成：交流电渗泵集成到MT中， ...更多信息在芯片上扭转并对齐微管。提出了一种基于非对称叉指电极的改进设计，以处理高导电缓冲层。为了可视化的泵浦效率，透明的平面微电极工艺开发使用铟锡氧化物（ITO）的沉积。静电平行板致动器，以引导流体运动也被开发出来。为探索微管自组装的可行性，研究了液滴在不同基底上干燥过程中微管的吸附情况，实现了生物功能分子分选和携带目标分子的生物杂化微系统.不同的靶分子，生物素-4-荧光素和兔抗小鼠IgG，选择性地附着在不同的珠（链霉亲和素包被的和蛋白A包被的珠）上。通过马达蛋白系统（珠粒上的驱动蛋白/芯片上的微管）单独转运靶分子，而没有任何液体操作，证明了多个靶分子在多个珠粒分选器上的可行性。少