Nanotransport device by integrating MEMS and biomolecular motors

该项目涉及MEMS和生物分子马达融合的纳米运输系统。这一目标是通过演示一种设备来实现的，该设备允许通过运动蛋白对珠子进行分类和运输。运动蛋白是沿着微管运动的运动蛋白。在将微管固定在装置中之前，需要对其进行定向。在流体微流动条件下，通过滑动实验进行定向。动力蛋白分子固定在微通道玻璃表面，微管通过动力蛋白运动进行运输。滑动微管的运动方向由通过微通道的压力驱动流控制，其极性在指定方向上取向。极性通过动力蛋白包被微球的单向输运来检测。定向成功率在80% ~ 91%之间。通过两种方法研究了MT定向流体流动的集成：将交流电渗透泵集成到芯片上，使微管更加倾斜和排列；针对高导电性缓冲电路，提出了一种基于非对称交叉电极的设计方案。为了可视化泵浦效率，采用氧化铟锡（ITO）沉积技术开发了透明平面微电极工艺。静电平行板作动器用于控制流体运动。成功地实现了高效稳定的驱动运行。为了研究微管自组装的可行性，研究了微管在不同基质上液滴干燥过程中的吸附。最后，实现了利用生物功能分子对靶分子进行分选和携带的生物混合微系统。不同的靶分子，生物素-4-荧光素和兔抗小鼠IgG，选择性地附着在不同的珠粒上（链亲和素包被和蛋白a包被珠粒）。靶分子由运动蛋白系统（运动蛋白在微球上/微管在芯片上）单独运输，无需任何液体操作，证明了多靶分子在多球分选器上的可行性。少

The project concerns nano transportation systems by fusion of MEMS and biomolecular motors. The goal was reached by demonstrating a device that permits the sorting and the transportation of beads by motor proteins. The motor proteins are kinesin moving along microtubules.The microtubules need to be oriented before their immobilization in the device. The orientation is performed by gliding assay under fluid microflow. Dynein molecules are immobilized on a microchannel glass surface and microtubules were transported by dynein motion. Moving directions of gliding microtubules were controlled by a pressure-driven flow through the microchannel, and their polarities were oriented in the designated direction. The polarities were checked by the unidirectional transport of kinesin-coated microspheres. Successful orientation yield were measured between 80% and 91%.The integration of fluid flow for the MT orientation was investigated by 2 approaches :An AC electro-osmotic pump was integrated to s … More teer and align microtubules on a chip. An adapted design, based on asymmetric interdigitated electrodes was develop to deal with high conductive buffer. To visualize the pumping efficiency, transparent planar microelectrode process was developed using Indium-Tin Oxide (ITO) deposition. Electrostatic parallel-plate actuators to steer the fluid motion were also developed. An efficient and stable actuation operation was been successfully achieved.To investigate the feasibility of microtubule self-assembly, the adsorption of microtubules was studied during the drying process of liquid droplets on various substrates.Finally, a bio-hybrid microsystem for sorting and carrying target molecules by bio-functional molecules have been realized. Different target molecules, biotin-4-fluorescein and Rabbit anti-mouse IgG, were selectively attached on different beads (Streptavidin-coated and protein A-coated beads). Target molecules were separately transported by the motor protein system (kinesin on beads / microtubules on chip) without any liquid manipulation proving the feasibility of multiple target molecules on multiple beads sorter. Less