权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Investigations of the molecular crowding effect on nucleic acid structures and development of nucleotide switches

分子拥挤效应对核酸结构和核苷酸开关开发的研究

基本信息

批准号：
17350087
负责人：
SUGIMOTO Naoki
金额：
$ 9.91万
依托单位：
Konan University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The purposes of this study are construction of a database for nucleotide interactions and development of functional nucleotides that can be applied for a molecular sensing in the non-aqueous media such as in a cell and on a sensor surface. We found the DNA hybridization energy on a gold nanoparticle differed from that obtained in a homogeneous aqueous solution. A model system that mimics the molecular crowding environment supposedly occurring in a cell and on a gold nanoparticle was constructed by using PEG (polyethylene glycol) and other cosolutes. A large amount of cosolutes added in an aqueous solution substantially decreases the water activity and large-sized cosolutes generate large excluded volume effect. Investigations of oligonucleotide structures and their thermodynamic parameters in the cosolute-containing solutions revealed that the molecular crowding by PEG or other cosolute decreased the stability of Watson-Crick base pairs but stabilized the triplex, G-quadruplex, and lef … More t-handed duplex (Z-form) structures. To understand the molecular crowding effect, we analyzed the thermodynamic and kinetic parameters and obtained the notations that water molecules participating in a nucleotide structure formation significantly influence the thermodynamic stability and the kinetics under the molecular crowding conditions. According to the nucleotide interaction data, we developed DNA molecules that changed the conformation when binding to polyamines or basic peptides, and those formed a DNA-wire by transferring into the molecular crowding media. Moreover, we constructed the DNA molecules with a logic gate function that responded to the molecular environment. We also demonstrated that the molecular crowding effect was used for up-or down-regulation of an enzyme activity and an efficient PCR amplification of a GC-rich DNA sequence. We also successfully synthesized several types of functional nucleotides. The researches that focus on the molecular crowding are very important from the standpoint of medical and engineering aspects. Less

本研究的目的是构建一个数据库的核苷酸相互作用和功能的核苷酸，可以应用于分子传感在非水介质中，如在细胞和传感器表面上的发展。我们发现在金纳米颗粒上的DNA杂交能量不同于在均匀水溶液中获得的能量。通过使用PEG（聚乙二醇）和其他共溶质构建了模拟细胞和金纳米颗粒上的分子拥挤环境的模型系统。在水溶液中加入大量的共溶质会显著降低水活度，并且大尺寸的共溶质会产生大的排阻体积效应。在含共溶质的溶液中，寡核苷酸结构及其热力学参数的研究表明，PEG或其他共溶质的分子拥挤降低了Watson-Crick碱基对的稳定性，但稳定了三链体、G-四链体和lef。 ...更多信息 t-手双螺旋（Z-型）结构。为了理解分子拥挤效应，我们分析了热力学和动力学参数，并得到的符号，水分子参与的核苷酸结构的形成显着影响的热力学稳定性和动力学条件下的分子拥挤。根据核苷酸相互作用的数据，我们开发了DNA分子，当结合到多胺或碱性肽时，它们改变了构象，并且通过转移到分子拥挤介质中形成DNA-线。此外，我们构建了具有逻辑门功能的DNA分子，该逻辑门功能对分子环境做出响应。我们还证明了分子拥挤效应用于上调或下调酶活性和高效PCR扩增富含GC的DNA序列。我们还成功地合成了几种类型的功能核苷酸。从医学和工程的角度来看，对分子拥挤的研究是非常重要的。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Development of functional nanobiodevices with DNAs

利用 DNA 开发功能性纳米生物器件

DOI：
发表时间：
2007
期刊：
影响因子：
0
作者：
Yasuko Nakamura;Yoko Nakano;Michiya Fujiki;三好大輔・中野修一・狩俣寿枝・井上真美子・中村かおり・魚谷有希・岡裕人・Z.-M. Wang・甲元一也・杉本直己
通讯作者：
三好大輔・中野修一・狩俣寿枝・井上真美子・中村かおり・魚谷有希・岡裕人・Z.-M. Wang・甲元一也・杉本直己

細胞内環境因子に応答するユニバーサルDNAロジックゲートの構築

构建响应细胞内环境因素的通用DNA逻辑门