Development of novel nucleic acid materials that efficiently work in the intracellular molecular environment

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We studied nucleic acid interactions and reactions by using a model experimental system that mimics the intracellular molecular environment. This research provided quantitative insights into influences of the molecular crowding medium on nucleic acid folding, a ribozyme activity, and interactions with proteins and peptides. It is supposed that these data can bridge the gap between the results obtained in vitro and in cells. Based on results regarding the effects of molecular environment, we developed novel nucleic acid materials and sensing methods that efficiently work under unusual conditions such as in living cells.