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Studies on the mechanism of rapid repair and degradation of post-ovulatory follicular tissues in the vertebrate ovary80197152

脊椎动物卵巢排卵后卵泡组织快速修复与降解机制研究80197152

基本信息

批准号：
17370021
负责人：
TAKAHASHI Takayuki
金额：
$ 9.95万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17370021/
关键词：
medaka ovary ovulation tissue repair protease 成長因子

项目摘要

The ovary is a reproductive organ whose main function is to generate germ cells (oocytes). In ovaries of mature vertebrate animals, a sequential event of follicular growth, ovulation, post-ovulatory follicular tissue repair and its subsequent degradation repeatedly occurs in a periodic manner unique for animal species. The aim of this study was to define the mechanism of rapid repair and degradation of the post-ovulatory follicular tissues in the vertebrate ovary. The medaka, a small fresh-water teleost, is a good experimental model to approach the problem because the fish ovulates everyday under appropriate culture conditions. Our findings in the research are as follows.1. We morphologically examined the structure of the follicle that had released its oocyte on ovulation. The post-ovulatory follicular tissue remaining in the ovary appeared to retain basic follicle layer structure for some time, but the tissue was found to degrade and disappear thereafter.2. Distribution of collagen type I and IV, which are markers for the follicular layer of the post-ovulatory follicular tissue, was examined. By in situ hybridization analysis, both collagens' mRNA was demonstrated to be localized to the follicle layer. Concomitant with disappearance of intact follicular layer structure, mRNA signals of the collagens became undetectable. Immunohistochimical analysis using specific antibodies for the collagens gave similar results.3. In attempts to search for proteases involved in such a degradation process, urokinase-type plasminogen activator (uPA) and gelatinase B, both of which exhibited ovary-specific expression in the medaka, were found to be candidate proteins. Plasmin, which is active protease generated from plasminogen by the action of uPA, and gelatinase B degraded various ECM proteins including collagens type I and IV in vitro.These results indicate that uPA/plasmin system and gelatinase B may play a role in the post-ovulatory rapid degradation process.

卵巢是生殖器官，其主要功能是产生生殖细胞(卵母细胞)。在成熟脊椎动物的卵巢中，卵泡生长、排卵、排卵后卵泡组织修复及其随后的退化的一系列事件以动物特有的周期性方式重复发生。本研究的目的是明确脊椎动物卵巢排卵后卵泡组织快速修复和降解的机制。青鱼是一种小型淡水硬骨鱼，是解决这个问题的一个很好的实验模型，因为这种鱼每天在适当的养殖条件下排卵。我们在研究中的发现如下。我们对排卵时释放卵母细胞的卵泡的结构进行了形态观察。结论：1.排卵后卵泡组织残留在卵巢内一段时间后，可保留基本卵泡层结构，但随后退化消失。检测排卵后卵泡组织滤泡层标志物I型和IV型胶原的分布。经原位杂交分析，两种胶原蛋白的mRNA均定位于卵泡层。随着完整的滤泡层结构的消失，胶原的mRNA信号变得不可检测到。用胶原蛋白的特异性抗体进行免疫组织化学分析，得到了类似的结果。在寻找参与这种降解过程的蛋白酶的过程中，尿激酶型纤溶酶原激活物(UPA)和明胶酶B被发现是候选蛋白，这两种蛋白都在青竹中表现出卵巢特异性的表达。纤溶酶是纤溶酶原在uPA作用下产生的活性酶，明胶酶B在体外降解包括I型和IV型胶原在内的多种ECM蛋白，这些结果表明uPA/纤溶酶系统和明胶酶B可能在排卵后的快速降解过程中起作用。

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Specificity of the medaka enteropeptidase serine protease and its usefulness as a biotechnological tool for fusion-protein cleavage

DOI：
10.1073/pnas.0610447104
发表时间：
2007-04-24
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
影响因子：
11.1
作者：
Ogiwara, Katsueki;Takahashi, Takayuki
通讯作者：
Takahashi, Takayuki

Function for proteinases in the ovulatory process.

蛋白酶在排卵过程中的功能。