Early evolution of eukaryotes inferred by combined phylogeny of multiple genes

通过多基因的联合系统发育推断真核生物的早期进化

• 批准号: 17370086
• 负责人: HASHIMOTO Tetsuo
• 金额: $ 9.09万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17370086/
• 关键词: eukaryotes; higher-order phylogeny; molecular phylogeny; maximun likelihood method; combined phylogeny; centrohelids; chromalyeolates hypothesis; fornicates

In order to elucidate a higher-order phylogeny of eukaryotes infer a root of the eukaryotic tree, combined phylogeny of multiple genes were carried out. For this purpose, several genes encoding cytosolic ribosomal RNAs and conservative proteins were isolated and sequenced from various organisms belonging to stramenopiles, alveolates, centrohelids, cryptomonads, rhodophytes, and fornicates. Including original sequence data obtained, various kinds of data sets with different taxon samplings and with different selections of genes were generated and these were analyzed by combined maximum likelihood analyses of the multiple genes. Until recently major outcomes listed below have been obtained:1. Optimal trees locating centrohelids at the outgroup position to bikont organisms and locating between the branches of amoebozoa and opisthokonts were independently obtained by different data sets, but these locations were not clearly supported with statistical confidence.2. No clear support was obtained for the monophyly of chromalveolates including cryptophytes, haptophytes, stramenopiles, and alveolates.3. Phylogenies of elongation factors 1 α and 2 demonstrated that Dysnectes brevis and a flagellate resemble to D. brevis (NY0173) were located between the branchings of trichomonads and diplomonads, supporting the previous results based on the SSUrRNA phylogeny.

为了阐明真核生物的高阶系统发育推断真核树的根，进行了多个基因的系统发育。为此，将编码胞质核糖体RNA和保守蛋白质的几个基因分离出来，并从属于stremonopiles，肺泡，centrohelids，Cryptomonads，Rhodophytes和Fornicates的各种生物体中进行测序。包括获得的原始序列数据，产生具有不同分类单元采样和不同基因选择的各种数据集，并通过多个基因的最大似然分析来分析这些数据集，并分析了这些数据集。直到最近，已经获得了下面列出的重大结果：1。最佳的树木位于比科特生物的外部位置，并位于变形虫分支和Opisthokonts之间的群体位置，并通过不同的数据集独立地获得，但是这些位置并未明确支持统计信心2。对于包括隐藻剂，happoytophytes，stremonopiles和alevaleolates的单一色脂蛋白的单植物，没有明确的支持。3。伸长因子1α和2的系统发育表明，与Brevis（NY0173）类似于Brevis和鞭毛酸盐（NY0173）位于Trichomonads和DiplomonAds的分支之间，支持先前基于基于ssurrna Perymen的结果。

项目成果

A close relationship between Cercozoa, and Foraminifera supported by phylogenetic analyses based on combined amino acid sequences of three cytoakeletal proteins,(actin,α-tubulin, . and β-tubulin)

基于三种细胞骨架蛋白（肌动蛋白、α-微管蛋白、. 和 β-微管蛋白）的组合氨基酸序列的系统发育分析支持尾虫和有孔虫之间的密切关系

• 发表时间: 2005
• 期刊: Gene 362
• 作者: Takishita;K.;Inagaki;Y.;Tsuchiya;M.;Sakaguchi;M.;and Maruyama;T.
• 通讯作者: T.

Ultrastructure and ribosomal RNA phylogeny of the free-living heterteoohic flagellate Dysnectis brevis n. gen., n. sp., a new member of the Fornicata

自由生活的异形鞭毛虫 Dysnectis brevis n 的超微结构和核糖体 RNA 系统发育。

• 发表时间: 2007
• 期刊: Journal of Eukaryotic Microbiolog 54
• 作者: Yubuki N;Inagaki Y;Nakayama T;Inouye I.
• 通讯作者: Inouye I.

Uktrastructyral and phylogenetic studies on Blastocysitis isolates from cocroaches

蟑螂囊胚炎分离物的超结构和系统发育研究

• 发表时间: 2007
• 期刊: Journal of Eukaryotic Microbiology 54
• 作者: Yoshikawa;H.;Wu;Z.;Howe;J.;Hashimoto;T.;Geok-Choo;N.;and Tan;K. S. W.
• 通讯作者: K. S. W.

Phylogeny of the Centrehelida inferred from SSUrRNA, tubulins and actin genes

从 SSUrRNA、微管蛋白和肌动蛋白基因推断的 Centrehelida 系统发育

• 发表时间: 2005
• 期刊: Journal of Molecular Evolution 61
• 作者: Sakaguchi;M.;Nakayama;T.;Hashimoto;T.;and Inouye;I.
• 通讯作者: I.

Ultrastructural and phylogenetic studies on Blastocystis isolates from cockroaches

• DOI: 10.1111/j.1550-7408.2006.00141.x
• 发表时间: 2007-01-01
• 期刊: JOURNAL OF EUKARYOTIC MICROBIOLOGY
• 影响因子: 2.2
• 作者: Yoshikawa, Hisao;Wu, Zhiliang;Tan, Kevin S. W.
• 通讯作者: Tan, Kevin S. W.