Compilation of the vibosome-related protein seguences and its application to the analysis on eukavyotic cellular evalution

病毒体相关蛋白序列的编译及其在真核细胞评价分析中的应用

• 批准号: 07680742
• 负责人: HASHIMOTO Tetsuo
• 金额: $ 1.47万
• 依托单位: THE INSTITUTE OF STATISTICAL MATHEMATICS
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680742/
• 关键词: ribosomal protein; molecular Evolution; alignment; molecular phylogenetic tree; eukaryotic cell; 下等真核生物

Large number of ribosomal protein (r-protein) sequences form various organisms have been accumulated up to now in the sequence databases. Although these data might have many informations on the evolution of the ribosomes, these are not well arranged in order because nomenclatures are various among different organisms, In this work we have systematically compiled the r-protein species, evolutionarily analyzing sequence similarities.On the basis of all the alignments constructed and phylogenetic analyzes carried out up to now, the evolutionary profile of r-proteins is emerging : (1) in no small number there are protein species that exist throughout the three primary kingdoms, (2) almost all the alignments show 'one to one' correlation to align regularly, that is irregular correlation such as internal transposition appears to be rarely found, (3) wide insertion/deletions and elongation in length are usually found in the comparisons among sequences from different primary kingdoms, while within a kingdom most of the evolutionary changes are amino acid replacements and minor insertion/deletions, (4) gene duplication events have occurred on several r-protein species in the various stages of eukaryotic evolution, (5) archaebactria do not show a monophyletic clade in no small number of r-protein species.On the other hand, we began to analyze the genes of ribosome-related molecules from amitochondriate protists, Glugea plecoglossi, Giardia lamblia and Trichomonas vaginalis to elucidate early evolution of eukaryotic ribosomes.

迄今为止，序列数据库中已经积累了大量的生物核糖体蛋白（r-protein）序列。尽管这些数据可能包含了许多关于核糖体进化的信息，但由于不同生物体的命名方法不同，这些信息并没有很好地按顺序排列。在这项工作中，我们系统地汇编了r-蛋白种类，进化地分析了序列相似性。在迄今为止构建的所有比对和进行的系统发育分析的基础上，r-蛋白的进化概况正在形成：（1）在不小的数目中，存在贯穿三个主要界的蛋白质种类，（2）几乎所有的比对显示“一对一”相关性以规则地比对，即不规则的相关性，例如内部转座似乎很少被发现，（3）在来自不同主要界的序列之间的比较中通常发现宽插入/缺失和长度的延长，而在一个界域内，大多数进化变化是氨基酸替换和微小的插入/缺失，（4）在真核生物进化的各个阶段，在几种r-蛋白物种上发生了基因复制事件，（5）古细菌在许多r-蛋白物种中没有显示出单系分支。另一方面，我们开始分析无线粒体原生生物Glugea plecoglossi、贾第鞭毛虫Giardia lamblia和毛滴虫Trichomonasvagularis的核糖体相关分子的基因，以阐明真核生物核糖体的早期进化。

项目成果

Yamamoto,A.,Hashimoto,T.,et al.: "Phylogenetic position of mitochondrion-lacking protozoan,Trichomonas tenax,based on amino acid sequences of elongation factors 1α and 2" Journal of Molecular Evolution. 44. 98-105 (1997)

Yamamoto, A., Hashimoto, T. 等人：“基于延伸因子 1α 和 2 的氨基酸序列，缺乏线粒体的原生动物 Trichomonas tenax 的系统发育位置”，分子进化杂志 44. 98-105 (1997)。 ）

Kamaishi,T.,Hashimoto,T.,Nakamura,Y.,Masuda,Y.,Nakamura,F.,Okamoto,K.,Shimizu,M.and Hasegawa,M.: "Complete nucleotide sequences of the genes encoding translation elongation factors 1α and 2 from a microsporidian parasite,Glugea plecoglossi: implications f

Kamaishi, T.、Hashimoto, T.、Nakamura, Y.、Masuda, Y.、Nakamura, F.、Okamoto, K.、Shimizu, M. 和 Hasekawa, M.：“编码翻译延伸的基因的完整核苷酸序列来自微孢子虫寄生虫 Glugea plecoglossi 的因子 1α 和 2：影响 f

Kamaishi,T.,Hashimoto,T.,et al.: "Protein phylogeny of translation elongation factor EF-1α suggests Microsporidians are extremely ancient eukaryotes" Journal of Molecular Evolution. 42. 257-263 (1996)

Kamaishi, T.、Hashimoto, T. 等人：“翻译延伸因子 EF-1α 的蛋白质系统发育表明微孢子虫是极其古老的真核生物”《分子进化杂志》42. 257-263 (1996)。

Kamaishi,T.,Hashimoto,T.,et al.: "Complete nuclectide sequences of the genes encoding translation elongation factors 1α and 2 from a microsporidian parasite,Glugea plecoglossi : implications for the deepest branching of eukaryotes" Journal of Biochemistry

Kamaishi, T.、Hashimoto, T. 等人：“来自微孢子虫寄生虫 Glugea plecoglossi 的编码翻译延伸因子 1α 和 2 的基因的完整核苷酸序列：对真核生物最深分支的影响”《生物化学杂志》

Mizuta,K.,Park,J.S.,et al.: "R1C1,a novel gene required for ribosome synthesis in Saccharomyces cerevisiae" Gene. (in press).

Mizuta,K.,Park,J.S.等人：“R1C1，酿酒酵母中核糖体合成所需的新基因”基因。