Clarification of the mechanisms involved in circadian body temperature rhythm and its physiological significance

阐明昼夜体温节律的机制及其生理意义

• 批准号: 17390062
• 负责人: NAGASHIMA Kei
• 金额: $ 9.75万
• 依托单位: Waseda University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390062/
• 关键词: thermoregulation; the suprachismatic nucle; brown adipose tissue; UCP1; Clock mutation; hypothalamus; cFos; daily torpor; 時計遺伝子; 体温; 温度感受性ニューロン

The goal of present study was to clarify the mechanism involved in generation of circadian body temperature rhythm. In the research, we hypothesized that 1) the suprachiasmatic nucleus (SCN), i. e. the central for circadian rhythms modulates thermosensitiveity at the levels of the central and periphery, and 2) the body temperature rhythm is important in synchronizing the rhythms of the central and periphery. In experiments, mice were exposed to the cold at 20℃ after ad-lib feeding or 48-h fasting, then we estimated metabolic heat production and UCP1 mRNA in the brown adipose tissue. In addition, cFos expression reflected by neural activity in the hypothalamus was also estimated. The same experimental protocol was repeated on Clock mutant mice, which lacks normal circadian rhythmicity. During ad-lib feeding, there were no differences in thermoregulatory responses and cFos expression in the cold. However, during fasting, heat production response was apparent only in the dark phase, and cFos expression in the SCN was augmented in the light phase. There were no phase-differences in the responses in Clock mutant mice. We also found an inhibitory network from the SCN and paraventricular nucleus associated with the sympathetic nerve activity. The SCN was influenced by environmental temperature and feeding condition, which is involved in time-dependency of body temperature regulation.

本研究的目的是阐明昼夜体温节律产生的机制。本研究假设：1）视交叉上核（SCN），即视交叉上核（SCN）; e.昼夜节律的中枢调节中枢和外周水平的温度敏感性，和2）体温节律在同步中枢和外周的节律中是重要的。在实验中，小鼠在自由进食或禁食48 h后暴露于20℃的寒冷环境中，然后检测代谢产热和棕色脂肪组织中UCP 1 mRNA的表达。此外，还估计了下丘脑中神经活动所反映的cFos表达。在缺乏正常昼夜节律的Clock突变小鼠上重复相同的实验方案。在自由进食期间，在冷的温度调节反应和cFos表达没有差异。然而，在禁食期间，产热反应仅在暗相明显，并且SCN中的cFos表达在亮相增强。Clock突变小鼠的反应没有相位差。我们还发现了一个来自SCN和室旁核的抑制网络，与交感神经活动有关。SCN受环境温度和摄食条件的影响，参与体温调节的时间依赖性。

项目成果

The median preoptic nucleus is involved in th facilitation of heat-escape/cold-seeking behavior during systemic salt loading in rats

正中视前核参与大鼠全身盐负荷期间热逃逸/寻冷行为的促进

• 发表时间: 2006
• 期刊: Journal of Physiology 292
• 作者: M. Konishi;et al.;K. Nagashima;K. Nagashima;K. Nagashima;M. Konishi
• 通讯作者: M. Konishi

Difference in thermoregulation between dark and light phase of fasted mice

禁食小鼠暗期和亮期体温调节的差异

• 发表时间: 2008
• 作者: K. Nagashima;et al.;内田有希;Y. Uchida;K. Nagashima;K. Tokizawa
• 通讯作者: K. Tokizawa

The influence of exercise induced dehydration on thermal sensation and comfort

运动性脱水对热感觉和舒适度的影响

• 发表时间: 2008
• 作者: K. Nagashima;et al.;内田有希;Y. Uchida;K. Nagashima;K. Tokizawa;Y. Uchida;K. Nagashima
• 通讯作者: K. Nagashima

The Influence of Exercise-Induced Dehydration or Thermal Sensation and Comfort

运动引起的脱水或热感觉和舒适度的影响

• 发表时间: 2008
• 作者: K;Tokizawa;Y. Uchida;K. Nagashima;K. Tokizawa;Y. Uchida;K. Nagashima;時澤健;内田有希;時澤健;内田有希;永島計;時澤健;時澤健;内田有希;Y. Uchida;K. Nagashima;K. Nagashima
• 通讯作者: K. Nagashima

体温調節、温冷感覚に与える性周期の影響

性周期对体温调节和热感觉的影响

• 发表时间: 2007
• 作者: K;Tokizawa;Y. Uchida;K. Nagashima;K. Tokizawa;Y. Uchida;K. Nagashima;時澤健;内田有希;時澤健;内田有希;永島計;時澤健;時澤健;内田有希;Y. Uchida;K. Nagashima;K. Nagashima;K. Tokizawa;Y. Uchida;K. Nagashima;K. Tokizawa;Y. Uchida;K. Tokizawa;Y. Uchida;K. Nagashima;K. Tokizawa;K. Tokizawa;Y. Uchida;K.Tokizawa;K.Nagashima;Kei Nagashima;Kei Nagashima;Mayumi Nakamura;Mayumi Nakamura;Kei Nagashima;永島 計;永島 計;中村 真由美;安 原祥
• 通讯作者: 安 原祥