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Development of a novel surgical procedure for large osteochondral defects using platelet rich plasma(PRP)

开发利用富血小板血浆（PRP）治疗大型骨软骨缺损的新型手术方法

基本信息

批准号：
17390408
负责人：
WADA Yuichi
金额：
$ 9.73万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The purpose of this study was to develop a new surgical technique for intra-articular osteochondral defects using porous hydroxyapatite (HA) including platelet rich plasma (PRP)-derived fibrin gel and scaffold-free cartilage tissue produced by the alginate-recovered-chondrocyte method (ARC method). First of all, we examined the effects of PRP-derived fibrin gel on the metabolism of cultured rabbit chondrocytes. The maturation process of the ARC tissue on porous HA block in vivo was also investigated. On the basis of these results, we implanted HA blocks with PRP (or platelet poor plasma, PPP) and the ARC tissue into the large osteochondral defects, and examined the effects of this procedure on bone and cartilage tissue repair. Three-dimensional cultivation of articular chondrocytes (embedded in alginate beads) on PRP-derived fibrin gel promoted their proliferation and total proteoglycan (PG) content in alginate bead, while PG content normalized with DNA content in alginate bead was significantly decreased by PRP gel When the ARC tissue was placed on porous HA block and implanted into subcutaneous pocket of athyrnir nude mice, the tissue was well integrated with the surface of Hablock. Maturation of the ARC tissue was observed in this condition within 8 weeks. Implantation of the HA-ARC tissue constructs with PRP gel into osteochondral defects enhanced cartilage repair and bone formation. These results suggest that combination of PRP gel with HA-ARC implantation can be a useful procedure for the repair of large osteochondral lesions.

本研究的目的是开发一种新的手术技术，用于关节内骨软骨缺损，使用多孔羟基磷灰石（HA），包括富血小板血浆（PRP）衍生的纤维蛋白凝胶和藻酸盐回收软骨细胞方法（ARC方法）产生的无支架软骨组织。首先，我们研究了PRP衍生的纤维蛋白凝胶对培养的兔软骨细胞代谢的影响。研究了ARC组织在多孔HA块上的体内成熟过程。在这些结果的基础上，我们将含有PRP（或贫血小板血浆，PPP）的HA块和ARC组织植入到大的骨软骨缺损中，并检查该过程对骨和软骨组织修复的影响。关节软骨细胞的三维立体培养（包埋在藻酸盐珠中）促进其增殖和藻酸盐珠中的总蛋白聚糖（PG）含量，而PRP凝胶显著降低藻酸盐珠中用DNA含量标准化的PG含量。该组织与Hablock的表面很好地结合。在这种条件下，在8周内观察到ARC组织的成熟。将具有PRP凝胶的HA-ARC组织构建体植入骨软骨缺损中增强了软骨修复和骨形成。这些结果表明，PRP凝胶与HA-ARC植入的组合可以是用于修复大型骨软骨病病变的有用程序。