Development of repair method for osteochondral defect using mesenchymal stem cells derived form umbilical cord blood

开发利用脐带血来源的间充质干细胞修复骨软骨缺损的方法

基本信息

  • 批准号:
    15591599
  • 负责人:
  • 金额:
    $ 2.24万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2003
  • 资助国家:
    日本
  • 起止时间:
    2003 至 2004
  • 项目状态:
    已结题

项目摘要

1.Mesenchymal stem cells are thought to be multipotential, capable of differentiating into multiple lineages. We attempted to characterize rat cells derived from fetal circulating blood (FCBCs) that displayed a fibroblastic morphology and differentiated into osteoblastic and chondrocytic lineages. Notably, they differentiated into a chondrocyte-specific phenotype on plastic culture dishes in medium supplemented only with 10% fetal bovine serum, without the use of a three-dimensional culture substrate. We also determined the adequet harvest period to get more FCBCs and measured the doubling time of FCBCs.2.Mesenchymal stem cells derived from bone marrow did not express COL2A1 or COL10A1 mRNA under the same culture conditions. FCBCs expressed two splice forms of type II collagen, IIA and IIB. Gene expression of type IIA in FCBCs was more than that in primary cultured rat rib cartilage derived chondrocytes, therefore, FCBCs was thought to be more immature than rib cartilage chondrocytes.3.Continuous culture promoted the maturation of chondrocytic cells differentiated from FCBCs.4.Passage culture decreased the ability of spontaneous differentiation of FCBCs.5.We evaluated the ability of spontaneous differentiation with frozen FCBCs. Frozen FCBCs maintained the ability and differentiated into osteoblastic and chondrocytic cells.6.FCBCs differentiated into adipogenic cells without 10% fetal bovine serum. These result showed that spontaneous differentiation ability of FCBCs was independent of the factors in the serum.7.We developed the type II collagen sponge as a scaffold for the repair of osteochondral defect.
1.间充质干细胞被认为是多潜能的,能够分化成多个谱系。我们试图表征来自胎儿循环血液(FCBCs)的大鼠细胞,这些细胞显示成纤维细胞形态并分化为成骨细胞和软骨细胞谱系。值得注意的是,它们在仅添加10%胎牛血清的塑料培养皿中分化为软骨细胞特异性表型,而不使用三维培养基质。我们还确定了适当的采收期以获得更多的FCBCs,并测量了FCBCs的倍增时间。在相同的培养条件下,骨髓间充质干细胞不表达COL2A1或COL10A1 mRNA。FCBCs表达两种II型胶原剪接形式IIA和IIB。IIA型基因在FCBCs中的表达高于在原代培养的大鼠肋软骨软骨细胞中的表达,因此,FCBCs被认为比肋软骨软骨细胞更不成熟。持续培养可促进fcbcs分化的软骨细胞成熟。传代培养降低了fcbcs的自发分化能力。我们评估了冷冻FCBCs的自发分化能力。冷冻后的FCBCs仍能分化成成骨细胞和软骨细胞。在不添加10%胎牛血清的情况下,FCBCs分化为成脂细胞。这些结果表明,FCBCs的自发分化能力不受血清因子的影响。我们研制了II型胶原海绵作为骨软骨缺损修复的支架材料。

项目成果

期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Spontaneous differentiation of mesenchymal stem cells obtained from fetal rat circulation
  • DOI:
    10.1016/j.bone.2004.05.006
  • 发表时间:
    2004-10-01
  • 期刊:
  • 影响因子:
    4.1
  • 作者:
    Naruse, K;Urabe, K;Itoman, M
  • 通讯作者:
    Itoman, M
骨・関節軟骨の保存
保护骨骼和关节软骨
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    占部憲;糸満盛憲
  • 通讯作者:
    糸満盛憲
Ken Urabe: "Determination of the completer cDNA sequence of rat type II collagen and evaluation of distinct expression patterns of type IIA and type IIB procollagen mRNAs during fracture repair in rats."J Orthop Sci. 8. 585-590 (2003)
Ken Urabe:“确定大鼠 II 型胶原蛋白的完整 cDNA 序列,并评估大鼠骨折修复过程中 IIA 型和 IIB 型前胶原 mRNA 的不同表达模式。”J Orthop Sci。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
関節の再生医学
关节再生医学
  • DOI:
  • 发表时间:
    2002
  • 期刊:
  • 影响因子:
    0
  • 作者:
    占部憲;糸満盛憲
  • 通讯作者:
    糸満盛憲
Preservation of bone and articular cartilage.
保存骨和关节软骨。
  • DOI:
  • 发表时间:
    2003
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ken Urabe;Moritoshi Itoman
  • 通讯作者:
    Moritoshi Itoman
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URABE Ken其他文献

URABE Ken的其他文献

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{{ truncateString('URABE Ken', 18)}}的其他基金

How dose the alteration in mechanical load due to change of alignment influence articular cartilage of knee joint
由于对准变化导致的机械负荷变化如何影响膝关节的关节软骨
  • 批准号:
    19591733
  • 财政年份:
    2007
  • 资助金额:
    $ 2.24万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)

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