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Study on olfactory regeneration by implantation of bone marrow cells or tissue stem cells

骨髓细胞或组织干细胞植入嗅觉再生的研究

基本信息

批准号：
17390461
负责人：
NISHIZAKI Kazunori
金额：
$ 6.08万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

1) To investigate whether bone marrow cells are capable of differentiating into olfactory cells, GFP mouse bone marrow cells were implanted transvenously into immunotolerant mice. Scattering GFP+OMP+ cells were observed in the olfactory epithelium of recipient mice. GFP+ cells were distributed vertically and the number was increasing temporally. It suggests that bone marrow derived cells were engrafed as olfactory stem cells and differentiated into olfactory cells.2) To investigate how bone marrow cells are involved in epithelium regeneration after insult to the olfactory epithelium, GFP mouse bone marrow cells were implanted transvenously or locally. Although GFP+ cells were increasing in the injured olfactory epithelium, these were not GFP+OMP+. It suggests that inflammatory microenvironment is advantageous in remodeling than in differentiation.3) For implantation of bone marrow cells destined to differentiate into olfactory stem cells, how Notch signal or prolactin influences the di … More fferentiation and regeneration/proliferation of the olfactory epithelium was investigated. Notch signal and prolaction play an important role in differentiation and regeneration/proliferation.4) To investigate if locally implanted bone marrow cells were engrafted and differentiated, the salivary glands were used because of easy techniques and evaluation. Considering the number of false positive cells, local transplantation using subculture technique is more effective for increasing a engraftment rate. GFP+ Aquaporin5 +cells may demonstrate that bone marrow cells can be engrafted in the parotid glands and then differentiate into acinar cells.5) To investigate whether bone marrow cells are able to differentiate pluripotently, the inner ear was observed after insult of an aminoglycoside. GFP+ cells were observed in the sensory epithelia of the inner ear.6) Whether G-CSF promotes an engraftment rate of bone marrow cells into the olfactory epithelium was investigate using mice insulted by methimazole. G-CSH is effective to increase an engraftment rate. Less

1)为了研究骨髓细胞是否能够分化为嗅觉细胞，将GFP小鼠骨髓细胞经皮植入免疫耐受小鼠。在受体小鼠的嗅上皮中观察到散在的GFP+OMP+细胞。GFP+细胞呈垂直分布，数量随时间增加。提示骨髓来源的细胞作为嗅干细胞植入并分化为嗅细胞。2）为了研究骨髓细胞如何参与嗅上皮损伤后的上皮再生，将GFP小鼠骨髓细胞经皮或局部植入。尽管GFP+细胞在损伤的嗅上皮中增加，但这些细胞不是GFP+OMP+。这表明炎症微环境在重建中比在分化中更有利。3）对于注定分化为嗅觉干细胞的骨髓细胞的植入，Notch信号或催乳素如何影响分化的分化， ...更多信息研究嗅上皮的分化和再生/增殖。Notch信号和增殖在分化和再生/增殖中起重要作用。4）为了研究局部植入的骨髓细胞是否植入并分化，使用唾液腺，因为其技术和评估简单。考虑到假阳性细胞的数量，使用继代培养技术的局部移植对于增加植入率更有效。GFP+水通道蛋白5+细胞可以证明骨髓细胞可以移植到腮腺中，然后分化成腺泡细胞。5）为了研究骨髓细胞是否能够多能分化，在氨基糖苷类损伤后观察内耳。在内耳感觉上皮中观察到GFP+细胞。6）使用甲巯咪唑损伤的小鼠研究G-CSF是否促进骨髓细胞向嗅上皮中的植入率。G-CSH可有效提高植入率。少