Multi-drug resistance strains in Bengal region and analysis of their resistance genes as compared with those in Japan

孟加拉地区多重耐药菌株及其耐药基因与日本比较分析

• 批准号: 17406012
• 负责人: YAMASAKI Shinji
• 金额: $ 8.58万
• 依托单位: OSAKA PREFECTURE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17406012/
• 关键词: Bengal region; Multiple-drug resistance; Antimicrobial agents; Diarrhea; Enteric bacteria

Overuse of antimicrobial agents has resulted in the emergence of multiple-drug resistance strains, which make it difficult to treat bacterial infections easily cured previously. The aim of study was conducted to analyze the rate of antimicrobial resistance strains isolated from severe diarrheal cases and the environment. The results obtained from Bengal region were also compared with those from Japan. In addition, what types of antimicrobial resistance genes present, particularly for integron, were analyzed in the isolates. Vibrio cholerae strains isolated in Kolkata, India in 2006 were mostly O1 serogroup in which Inaba serotype was dominant. Analysis of antimicrobial susceptibility revealed that most of the strains were multiple-drug resistance. More than 90% were resistant to ampicillin, streptomycin, neomycin, nalidixic acid, and erythromycin. On the other hand, 93% were susceptible to tetracycline and 50% were resistant to ciprofloxacin. Recently some of the NAG vibrio were found … More to be misidentified and now turned out to be V.fluvialis. Analysis of 19 multiple-drug resistant strains of V.fluvialis exhibited that class 1 integron and SXT element were detected in 9 and 4 strains of V.fluvialis, respectively. Drug efflux pump was involved in the fluoroquinolone resistance of all the fluoroquinolone resistant strains. Furthermore distribution of integrase (intl) gene in the environment was examined by PCR. The results indicated that intl1 and intl2 genes were widely distributed in the environment. In addition, SXT element was also detected in some cases. Particularly, in Bangladesh, isolation rate of V.cholerae O1 increased when streptomycin, sulfamethoxazole and trimethoprim, whose resistant genes are encoded on SXT element, were included in the culture medium. In the case of Japan, however, resistant rates of diarrheagenic Escherichia coli and Campklobacters isolated from diarrheal patients were relatively low in comparison to those in India and Bangladesh. For example, less than 10% of diarrheagenic E.coli and Campylobacters were resistant to ciprofloxacin. Intl1 and intl2 genes were rarely detected in enrichment culture of stool specimen of diarrheal patients and Intl3 gene was not detected at all. However, intl1 and intl2 genes were detected in enrichment culture of the environmental samples such as river water and meat but SXT element was not detected in enrichment culture of the environmental samples.These data indicated that integron is widely distributed not only in Bengal region but also in Japan such in the environmental water and meat. However, distribution of integron in the stool specimen in Bengal region was much higher than that in Japan. Furthermore, the rate of multiple-drug resistance as well as fluoroquinolone resistance strains isolated from patients was higher in Bengal region than in Japan. This could be explained by the easy purchase of the antimicobial agents at the pharmacy without prescription in Bengal region. Less

抗菌药物的过度使用导致了多重耐药菌株的出现，这使得以前容易治愈的细菌感染难以治疗。本研究的目的是分析重症腹泻病例中分离的抗菌药物耐药率和环境因素。并将孟加拉地区的结果与日本的结果进行了比较。此外，还分析了菌株中存在哪些类型的抗微生物药物抗性基因，特别是整合子。2006年在印度加尔各答分离的霍乱弧菌菌株以O1血清型为主，稻叶血清型占主导地位。药敏分析显示，大多数菌株具有多重耐药。90%以上的患者对氨苄西林、链霉素、新霉素、萘啶酸和红霉素耐药。另一方面，93%的患者对四环素敏感，50%的患者对环丙沙星耐药。最近发现了一些NAG弧菌…更多的被错误识别，现在被证明是河流弧菌。对19株多重耐药流感弧菌的分析表明，在9株和4株流感弧菌中分别检测到1类整合子和SXT元件。药物外排泵参与了所有耐药菌株对氟喹诺酮类药物的耐药过程。利用PCR检测整合酶基因在环境中的分布。结果表明，int1和intl2基因在环境中分布广泛。此外，在某些情况下还检测到SXT元素。特别是在孟加拉国，当将抗性基因编码在SXT元件上的链霉素、磺胺甲恶唑和甲氧苄啶加入培养基时，霍乱弧菌O1的分离率提高了。然而，在日本，与印度和孟加拉国相比，从腹泻患者中分离出的致泻性大肠杆菌和坎氏杆菌的耐药率相对较低。例如，不到10%的致泻性大肠杆菌和弯曲杆菌对环丙沙星耐药。腹泻患者粪便标本富集培养中很少检测到int1和intl2基因，而Intl3基因则完全未检测到。而在环境样品如河水和肉类的富集培养中检测到int1和intl2基因，而在环境样品的富集培养中未检测到SXT元素。这些数据表明，整合子不仅在孟加拉地区广泛分布，而且在日本的环境水和肉类中也广泛分布。然而，孟加拉地区粪便标本中整合子的分布远高于日本。此外，从患者中分离出的多重耐药和氟喹诺酮类耐药菌株的比例在孟加拉地区高于日本。这可能是由于在孟加拉地区，在没有处方的情况下很容易在药店购买抗菌药物。少

项目成果

Unnoticed Spread of Class 1 Integrons in Gram‐Positive Clinical Strains Isolated in Guangzhou, China

• DOI: 10.1111/j.1348-0421.2006.tb03815.x
• 发表时间: 2006-06
• 期刊: Microbiology and Immunology
• 影响因子: 2.6
• 作者: Lei Shi;Mei-Shu Zheng;Zenghuang Xiao;M. Asakura;Jianyu Su;Lin Li;S. Yamasaki

Mechanisms of drug resistance in clonally related isolates of Vibrio fluvialis isolated in Kolkata. India.

加尔各答分离的河弧菌克隆相关分离株的耐药机制。

• 发表时间: 2006
• 期刊: Antimicrobial Agents and Chemotherapy 50 (7)
• 作者: Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.;Faruque S.M. et al.;Srinivasan V.B. et al.
• 通讯作者: Srinivasan V.B. et al.

Distribution and characterization of integrons in various serogroups of Vibrio cholerae strains isolated from diarrheal patients between 1992 and 2000 in Kolkata, India.

1992 年至 2000 年印度加尔各答腹泻患者分离的霍乱弧菌不同血清群中整合子的分布和特征。

• 发表时间: 2006
• 期刊: Journal of Medical Microbiology 55 (5)
• 作者: Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.
• 通讯作者: Shi L.et al.

Distribution and characterization of integrons in various serogroups of Vibrio cholerae isolated from diarrheal patients between 1992 and 2000 in Kolkata, India

1992 年至 2000 年印度加尔各答腹泻患者分离的霍乱弧菌不同血清群中整合子的分布和特征

• 发表时间: 2006
• 期刊: Journal of Medical Microbiology in press
• 作者: Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.;Shi L.et al.;Faruque S.M. et al.;Srinivasan V.B. et al.;Taguchi M. et al.;Shi L.et al.;Srinivasan V.B. et al.;Taguchi M et al.;Singh S. et al.;Srinivasan V.B. et al.;Nair G.B.et al.;Taguchi M. et al.;Shi L.et al.;Shi L.et al.
• 通讯作者: Shi L.et al.

The potent antibacterial activity of sitafloxacin against fluoroquinolone-resistant clinical isolates of Vibrio cholerae 01

西他沙星对氟喹诺酮耐药临床分离株霍乱弧菌 01 的有效抗菌活性

• 发表时间: 2007
• 期刊: Microbiology and Immunology 51(4)
• 作者: Keiko;Wada et al.;Hinenoya A. et al.;Okuda J. et al.;Hinenoya A. et al.;Okuda J. et al.
• 通讯作者: Okuda J. et al.