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Development of system to recover heavy metal from ore, polluted soil and wastewater by using iron-oxidizing bacterium

利用铁氧化细菌从矿石、污染土壤和废水中回收重金属系统的开发

基本信息

批准号：
17510069
负责人：
TAKEUCHI Fumiaki
金额：
$ 2.59万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Iron-oxidizing bacterium, Acidithiobacillus ferrooxidans, is one of the most important bacteria for the bioleaching of copper and gold ores. In order to use the mercury reducing activity of A. ferrooxidans for the bioremediation of mercury, mercury-resistant A. ferrooxidans strains SUG 2-2 and MON-1 were screened among 150 strains of iron-oxidizing bacteria isolated from natural environments. It was found that strains SUG 2-2 and MON-1 have a novel ferrous iron-dependent mercury volatilization activity as well as an NADPH-dependent mercury reductase activity. Strain MON-I has volatilization activity an organomercurial lyase-like activity by cytochrome c oxidase. Nearly 100% of the total mercury in mercury-polluted soil or mercury wastewater was volatilized and recovered by incubating SUG 2-2 or MON-1 cells. suggesting that these mercury-resistant strains can be used for the bioremediation of inorganic and organic mercurial compounds.A. ferrooxidans strain D3-2, which has a high copper bioleaching activity, was isolated from a low-grade sulfide are dump in Chile. Since sulfite ion is a highly toxic intermediate produced during sulfur oxidation that strongly inhibits iron oxidase activity, these results confirm hat strain D3-2, with a unique sulfite resistant-sulfite oxidase, was able to solubilize more copper from chalcopyrite than strain ATCC 23270, with a sulfite-sensitive sulfite oxidase. An electric cultivation device was developed. This was useful for the enzymatic refinement and the Applied technology.

氧化铁硫杆菌是细菌浸出铜金矿最重要的细菌之一。为了利用氧化亚铁假单胞菌的汞还原活性进行汞的生物修复，从150株自然环境中分离到的氧化亚铁链霉菌中筛选出耐汞的氧化亚铁假单胞菌SUG2-2和MON-1。研究发现，菌株SUG2-2和MON-1具有依赖亚铁的汞挥发活性和依赖NADPH的汞还原酶活性。菌株MON-I具有挥发活性和与细胞色素C氧化酶类似的有机汞裂解酶活性。通过培养SUG2-2或MON-1细胞，汞污染土壤或含汞废水中近100%的总汞被挥发和回收。表明这些耐汞菌株可用于无机和有机汞化合物的生物修复。氧化亚铁杆菌D3-2菌株是从智利一个低品位硫化物垃圾场中分离到的一株具有较高浸出活性的氧化亚铁细菌。由于亚硫酸盐离子是硫磺氧化过程中产生的一种剧毒中间体，对铁氧化酶活性有强烈的抑制作用，这些结果证实了菌株D3-2具有独特的抗亚硫酸盐亚硫酸盐氧化酶，能够比菌株ATCC 23270利用亚硫酸盐敏感的亚硫酸盐氧化酶从黄铜矿中溶解更多的铜。研制了一种电动耕作装置。这对酶法精制和应用技术具有一定的参考价值。