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Development of system to recover heavy metal from ore, polluted soil and wastewater by using iron-oxidizing bacterium

利用铁氧化细菌从矿石、污染土壤和废水中回收重金属系统的开发

基本信息

批准号：
17510069
负责人：
TAKEUCHI Fumiaki
金额：
$ 2.59万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

Iron-oxidizing bacterium, Acidithiobacillus ferrooxidans, is one of the most important bacteria for the bioleaching of copper and gold ores. In order to use the mercury reducing activity of A. ferrooxidans for the bioremediation of mercury, mercury-resistant A. ferrooxidans strains SUG 2-2 and MON-1 were screened among 150 strains of iron-oxidizing bacteria isolated from natural environments. It was found that strains SUG 2-2 and MON-1 have a novel ferrous iron-dependent mercury volatilization activity as well as an NADPH-dependent mercury reductase activity. Strain MON-I has volatilization activity an organomercurial lyase-like activity by cytochrome c oxidase. Nearly 100% of the total mercury in mercury-polluted soil or mercury wastewater was volatilized and recovered by incubating SUG 2-2 or MON-1 cells. suggesting that these mercury-resistant strains can be used for the bioremediation of inorganic and organic mercurial compounds.A. ferrooxidans strain D3-2, which has a high copper bioleaching activity, was isolated from a low-grade sulfide are dump in Chile. Since sulfite ion is a highly toxic intermediate produced during sulfur oxidation that strongly inhibits iron oxidase activity, these results confirm hat strain D3-2, with a unique sulfite resistant-sulfite oxidase, was able to solubilize more copper from chalcopyrite than strain ATCC 23270, with a sulfite-sensitive sulfite oxidase. An electric cultivation device was developed. This was useful for the enzymatic refinement and the Applied technology.

氧化亚铁硫杆菌（Acidithiobacillusferrooxidans）是一种重要的铜金矿石细菌浸出菌。为了利用A.铁氧化物对汞的生物修复，抗汞A.从自然界分离的150株铁氧化细菌中筛选出氧化亚铁细菌SUG 2-2和MON-1。发现菌株SUG 2-2和MON-1具有新的亚铁依赖性汞挥发活性以及NADPH依赖性汞还原酶活性。菌株MON-I具有挥发活性和类细胞色素c氧化酶的有机汞裂解酶活性。通过培养SUG 2-2或MON-1细胞，汞污染土壤或含汞废水中的总汞几乎100%被挥发和回收。表明这些抗汞菌株可用于无机和有机汞化合物的生物修复。从智利某低品位硫化矿堆中分离到一株具有较高浸铜活性的氧化亚铁细菌D3-2。由于亚硫酸根离子是硫氧化过程中产生的强烈抑制铁氧化酶活性的高毒性中间体，因此这些结果证实了具有独特的抗亚硫酸盐-亚硫酸盐氧化酶的菌株D3-2能够比具有亚硫酸盐敏感性亚硫酸盐氧化酶的菌株ATCC 23270从黄铜矿中溶解更多的铜。研制了电动栽培装置。这对酶法精制和应用技术具有一定的指导意义。