Development of system to recover heavy metal from ore, polluted soil and wastewater by using iron-oxidizing bacterium

利用铁氧化细菌从矿石、污染土壤和废水中回收重金属系统的开发

• 批准号: 17510069
• 负责人: TAKEUCHI Fumiaki
• 金额: $ 2.59万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17510069/
• 关键词: iron oxidizing bacterium; Acidithiohacillus ferrooxidans; Bioleaching; Rioremediation; Mercury-Resistant Strains; Electrochemical cultivation; Mercury-polluted soil treatment; Conner bioleaching

Iron-oxidizing bacterium, Acidithiobacillus ferrooxidans, is one of the most important bacteria for the bioleaching of copper and gold ores. In order to use the mercury reducing activity of A. ferrooxidans for the bioremediation of mercury, mercury-resistant A. ferrooxidans strains SUG 2-2 and MON-1 were screened among 150 strains of iron-oxidizing bacteria isolated from natural environments. It was found that strains SUG 2-2 and MON-1 have a novel ferrous iron-dependent mercury volatilization activity as well as an NADPH-dependent mercury reductase activity. Strain MON-I has volatilization activity an organomercurial lyase-like activity by cytochrome c oxidase. Nearly 100% of the total mercury in mercury-polluted soil or mercury wastewater was volatilized and recovered by incubating SUG 2-2 or MON-1 cells. suggesting that these mercury-resistant strains can be used for the bioremediation of inorganic and organic mercurial compounds.A. ferrooxidans strain D3-2, which has a high copper bioleaching activity, was isolated from a low-grade sulfide are dump in Chile. Since sulfite ion is a highly toxic intermediate produced during sulfur oxidation that strongly inhibits iron oxidase activity, these results confirm hat strain D3-2, with a unique sulfite resistant-sulfite oxidase, was able to solubilize more copper from chalcopyrite than strain ATCC 23270, with a sulfite-sensitive sulfite oxidase. An electric cultivation device was developed. This was useful for the enzymatic refinement and the Applied technology.

铁氧化细菌，酸硫胆杆菌铁氧化物是铜和金矿的生物素质最重要的细菌之一。为了利用汞曲霉的汞降低活性进行汞的生物修复，在150种从自然环境中分离出的150种铁氧化细菌中，筛选了耐汞的抗汞曲霉菌株SUG 2-2和MON-1。发现菌株SUG 2-2和MON-1具有一种新型的亚铁依赖性汞挥发活性以及NADPH依赖性的汞还原酶活性。菌株MON-1具有挥发活性，一种细胞色素C氧化酶的有机裂解酶样活性。通过孵育2-2或MON-1细胞，将汞粉污染土壤或汞废水总汞总数的近100％挥发并回收。表明这些耐汞菌株可用于生物修复无机和有机汞化合物。从低级硫化物中分离出具有高铜生物无能活性的Ferrooxidans菌株D3-2在智利中分离出来。由于亚硫酸盐离子是在硫氧化过程中产生的一种剧毒中间体，强烈抑制铁氧化酶活性，因此这些结果证实了帽子D3-2具有独特的硫酸盐抗性硫酸盐氧化酶，能够使与硫酸菌株的硫酸含量更高的铜溶解能力，而硫酸含量是ATCC 23270，并具有硫酸含量为23270。开发了电耕作装置。这对于酶促的完善和应用技术很有用。

项目成果

テトラチオン酸で生育した鉄酸化細菌Acidithiobacillus ferrooxidans ATCC 23270株の二価鉄生成酵素活性無機並びに有機水銀分解機構の解明

阐明在连四硫酸上生长的铁氧化细菌 Acidithiobacillusferrohabitans ATCC 23270 的二价铁产生酶活性的无机和有机汞分解机制

• 发表时间: 2008
• 作者: 杉尾 剛;タハターヘル;金尾忠芳;竹内文章
• 通讯作者: 竹内文章

硫黄培地に増殖したAcidithobacillus ferooxidans MON-1株の無機水銀及び有機水銀分解活性

硫磺培养基中氧化亚铁酸杆菌MON-1菌株的无机和有机汞分解活性

• 发表时间: 2007
• 作者: 杉尾 剛;中村壮作;金尾忠芳;竹内文章
• 通讯作者: 竹内文章

チリ銅鉱山から単利したA. ferrooxidans D3-2株の銅溶出に係わる特性

智利铜矿获得的氧化亚铁 A. 氧化亚铁 D3-2 菌株的铜洗脱特性

• 发表时间: 2006
• 作者: 若林雅則;金尾忠芳;上村一雄;竹内文章;杉尾 剛
• 通讯作者: 杉尾 剛

Growth Inhibition by Tungsten in the Sulfur-Oxidizing Bacterium Acidithiobacillus thiooxidans

• DOI: 10.1271/bbb.69.2073
• 发表时间: 2005-01
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: A. Negishi;Tadashi Muraoka;T. Maeda;F. Takeuchi;T. Kanao;K. Kamimura;T. Sugio

Preparation of plasma membranes which has iron-and sulfur-oxidizing activities from sulfur-grown Acidithobacillus ferrooxidans ATCC 23270

由硫生长的氧化亚铁酸杆菌 ATCC 23270 制备具有铁和硫氧化活性的质膜

• 发表时间: 2007
• 作者: Mohamed TAHA;Taher;Tadayoshi KANAO;Fumiaki TAKEUCHI and Tsuyoshi SUGIO
• 通讯作者: Fumiaki TAKEUCHI and Tsuyoshi SUGIO