Volatilization of Metal Mercury from Inorganic and Organic Mercury by Highly Mercury-Resistant Acidithiobacillus ferrooxidans and its Application to Bioremediation

高耐汞氧化亚铁硫杆菌对无机汞和有机汞中金属汞的挥发及其在生物修复中的应用

• 批准号: 21510086
• 负责人: TAKEUCHI Fumiaki
• 金额: $ 3万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21510086/
• 关键词: 鉄酸化細菌; Acidithiobacillus ferrooxidans; 高度水銀耐性株; 水銀気化活性; 酵素解析; Cytochrome c oxidas; 電気培養; Bioremediation; ctochrome coxidas; 環境浄化; 微生物固定化; Acidithiobacillus ferrooaidans

Acidithiobacillus ferrooxidans MON-1 is highly resistant not only to mercuric chloride but also to organomercurials. It have a novel ferrous iron-dependent mercury volatilization activity as well as an NADPH-dependent mercury reductase activity, We have found that cytochrome c oxidase, purified from strain MON-1, reduces HgCl_2 to volatilizable metal mercury(Hg^0) with reduced mammalian cyt. c or Fe^<2+> as an electron donor. In this study we found that cyt. c oxidase can volatilize Hg^0 from CH_3HgCl(MMC) as well as from HgCl_2 with reduced mammalian cyt. c or c-type cyt. c oxidase purified from strain MON-1 as an electron donor. We also found that MMC volatilization activity is present in the MON-1 plasma membrane but not in the cytosol. These activities were strongly inhibited by NaCN and the antibody produced against purified MON-1 cyt. c oxidase.

酸硫代杆菌铁氧化物Mon-1不仅对氯化汞，而且对有机核心具有高度抗性。它具有一种新型的亚铁依赖性汞挥发活性以及NADPH依赖性的汞还原酶活性，我们发现，从菌株MON-1纯化的细胞色素C氧化酶将HGCL_2纯化为可挥发的金属汞（HG^0），并具有还原的哺乳动物细胞。 C或Fe^<2+>作为电子供体。在这项研究中，我们发现了Cyt。 C氧化酶可以从CH_3HGCL（MMC）以及减少哺乳动物Cyt的HGCL_2挥发Hg^0。 C或C型Cyt。 C氧化酶从菌株MON-1纯化为电子供体。我们还发现，MMC挥发活性存在于MON-1质膜中，但在细胞质中不存在。 NACN和针对纯化的MON-1 CYT产生的抗体极大地抑制了这些活性。 C氧化酶。

项目成果

Reduction of cytochrome c by tetrathionate in the presence of tetrathionate hydrolase purified from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270

在从硫生长的氧化亚铁硫杆菌 ATCC 23270 中纯化的连四硫酸盐水解酶存在下，连四硫酸盐还原细胞色素 c

• 发表时间: 2009
• 作者: Taha;T.M.;F.Takeuchi;T.Sugio
• 通讯作者: T.Sugio

高度水銀耐性鉄酸化細菌Acidithobacillus ferooxidans MON-1株由来aa_3型cytochrome c oxidaseによる有機水銀の分解

高汞抗性铁氧化菌嗜酸杆菌MON-1菌株aa_3型细胞色素c氧化酶分解有机汞

• 发表时间: 2009
• 作者: 杉尾剛;薦田友恵;岡崎夕子;竹田有希;ターヘルタハ;竹内文章
• 通讯作者: 竹内文章

Volatilization and Recovery of Mercury from Mercury Wastewater Using Acidithiobacillus ferrooxidans MON-l Strain Immobilized on Porous Ceramic

多孔陶瓷固定化氧化亚铁硫杆菌MON-l菌株从汞废水中挥发和回收汞

• 发表时间: 2009
• 期刊: PROCEEDINGS THE 5TH VIETNAMESE JAPANESE SCIENTIFIC EXCHANGE CONFERENCE, OCTOBER
• 作者: Tong Thi My Thi;Fumiaki TAKEUCHI and Tsuyoshi SUGIO
• 通讯作者: Fumiaki TAKEUCHI and Tsuyoshi SUGIO

鉄酸化細Acidithiobacillus ferrooxidansにおけるグルタチオン非依存性,元素硫黄依存性の亜硫酸生成活性の存在

铁氧化剂氧化亚铁硫杆菌中存在不依赖于谷胱甘肽、依赖于元素硫的亚硫酸盐生产活性

• 发表时间: 2011
• 作者: 杉尾剛;小野田桂子;竹内文章
• 通讯作者: 竹内文章

Ferrous Iron Production Mediated by Tetrathionate Hydrolase in Tetrathionate-, Sulfur-, and Iron-Grown Acidithiobacillus ferrooxidans ATCC 23270 Cells

• DOI: 10.1271/bbb.90036
• 发表时间: 2009-06
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: T. Sugio;T. Taha;F. Takeuchi