Analyses of visual pigments and G proteins coupled with them in light-sensitive chromatophores of teleost fishes

硬骨鱼光敏色素细胞中视觉色素和 G 蛋白的分析

• 批准号: 17570068
• 负责人: OSHIMA Noriko
• 金额: $ 2.03万
• 依托单位: Toho University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17570068/
• 关键词: light-sensitive chromatophores; visual pigment; Nile tilapia erythrophores; neon tetra iridophores; cyclic AMP; G protein; Gi; opsin; 光の波長; ティラピア赤色素胞; 婚姻色; サイクリックAMP; cAMP; PDE; ナイルティラピア赤色素胞; cGMP

1. In Nile tilapia erythrophores, pigment aggregation is caused by light at 400 and 600 nm and dispersion occurred in response to light at 500 nm. We found the expression of mRNAs of cone opsins, red, green and blue, in the chromatophores. Further neon tetra iridophores present in the lateral stripe skin are also light-sensitive, and light near 500 nm is most effective. RT-PCR showed the expression of mRNAs encoding rhodopsin and two kinds of cone opsins (Pi-green 1 and Pi green 2) in neon tetra skin where the iridophores exist. These mRNAs are also expressed in the lateral eyes. The cone opsin genes, Pi-green 1 and Pi-green 2, show high similarity with the g101 and g102 genes of unique green cone opsins (belonging to the MWS/LWS group) of the blind Mexican cavefish The identity of amino acid sequences between Pi-green 1 and Pi-green 2 is considered to be more than 90% The red opsin gene was not expressed in neon tetra skin.2. Using anti-opsin (MWS/LWS) and anti-rhodopsin antibodies, western blotting analysis also demonstrated the presence of opsins in the skin where iridophores exist, and the localization of the opsins in iridophores was confirmed by immunohistochemical analysis.3. The involvement of cyclic AMP in the phototransduction was indicated using various inhibitors and activators of enzymes participating in the transduction. That is, light stimulation was accompanied by the decrease in cAMP level in the light-sensitive chromatophores. The treatment of Nile tilapia etythrophores and neon tetra iridophores with bacteria toxins also suggested the important role of Gi in the phototransduction.4. We also confirmed the expression of Gi mRNA and Gi protein in neon tetra iridophores by RT-PCR and western blotting respectively. Although partial expression of Gt mRNA was confirmed, Gt protein was not detected in the iridophores of neon tetra.

1. 在尼罗罗非鱼红细胞中，色素聚集是由 400 和 600 nm 的光引起的，而响应于 500 nm 的光而发生分散。我们发现色素细胞中存在红色、绿色和蓝色视锥细胞视蛋白 mRNA 的表达。另外，存在于侧条纹皮肤中的氖四虹彩细胞也对光敏感，并且接近 500 nm 的光是最有效的。 RT-PCR显示在存在虹彩细胞的neon tetra皮肤中编码视紫红质和两种视锥蛋白(Pi-green 1和Pi green 2)的mRNA的表达。这些 mRNA 也在侧眼中表达。视锥细胞视蛋白基因 Pi-green 1 和 Pi-green 2 与墨西哥盲穴鱼独特的绿色视锥细胞视蛋白（属于 MWS/LWS 组）的 g101 和 g102 基因具有高度相似性 Pi-green 1 和 Pi-green 2 之间的氨基酸序列同一性被认为超过 90% Neon tetra 中不表达红色视蛋白基因 皮肤.2.使用抗视蛋白(MWS/LWS)和抗视紫红质抗体，蛋白质印迹分析也证明了存在虹膜细胞的皮肤中存在视蛋白，并且通过免疫组化分析证实了视蛋白在虹膜细胞中的定位。3．使用参与转导的酶的各种抑制剂和激活剂表明环AMP参与光转导。也就是说，光刺激伴随着光敏感色素细胞中cAMP水平的降低。用细菌毒素处理尼罗罗非鱼红细胞和氖四虹细胞也表明Gi在光转导中的重要作用。4．我们还分别通过RT-PCR和蛋白质印迹证实了氖四虹彩细胞中Gi mRNA和Gi蛋白的表达。尽管证实了 Gt mRNA 的部分表达，但在 neon tetra 的虹膜细胞中未检测到 Gt 蛋白。

项目成果

Color and distribution of pigment cells in the skin of the zebrafish

斑马鱼皮肤色素细胞的颜色和分布

• 发表时间: 2006
• 期刊: Journal of SJWS 6
• 作者: K. Miyaji;M. Kimura;N. Oshima
• 通讯作者: N. Oshima

ネオンテトラ運動性虹色素胞における光の直接受容とそのシグナル伝達について

氖四运动虹彩细胞中的直接光接收和信号转导

• 发表时间: 2006
• 作者: A.;Kasai;N.;Oshima;河西 亜希子・大島 範子;河西 亜希子・大島 範子
• 通讯作者: 河西 亜希子・大島 範子

Study on the phototransduction in motile iridohpores of the neon tetra

氖四运动虹膜孔光转导的研究

• 发表时间: 2006
• 作者: A.;Kasai;N.;Oshima
• 通讯作者: Oshima

Study on the mechanism of light-reception in motile iridohpores in dermis of the neon tetra

霓虹灯真皮层活动虹膜孔感光机制的研究

• 发表时间: 2005
• 作者: A.;Kasai;N.;Oshima
• 通讯作者: Oshima

The signaling pathway in photoresponses that may be mediated by visual pigments in erythrophores of Nile tilapia

• DOI: 10.1111/j.1600-0749.2005.00267.x
• 发表时间: 2005-10-01
• 期刊: PIGMENT CELL RESEARCH
• 作者: Ban, E;Kasai, A;Oshima, N
• 通讯作者: Oshima, N