Evolution of Globin and Hatching Enzyme Genes in Teleosts

硬骨鱼球蛋白和孵化酶基因的进化

• 批准号: 17570189
• 负责人: IUCHI Ichiro
• 金额: $ 2.58万
• 依托单位: Sophia University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17570189/
• 关键词: Gene; Genome; Evolution; Protein; Hemoglobin; Hatching Enzyme; 卵化酵素; 蛋白質; グロビン; メダカ; 硬骨魚

In the present research project, we clarified three characteristics of globin gene structure and expression in medaka: 1) head-to-head orientation of major α and β globin genes, 2) existence of cis-regulatory regions responsible for spatio-temporal expression of the genes 1Kb-5'upstream to the respective genes, 3) localization of the globin genes in 2 clusters on 2 chromosomes. Molecular phylogenetic analysis showed that all the teleostean globin genes were assigned into 4 groups, and in the medaka genome, the genes for one of the 4 that functions as an adult globin were pseudogenized during evolution.In addition, we identified hatching enzyme genes in the genomes of about 30 teleosts. The results showed that the hatching enzyme genes in fishes belonging to Elopomorpha were ancestral, and were diversified into 2 groups of genes in the descendants Otocephara and Euteleostei. Especially, the EICE genes of Euteleostei would be established by "phylogeny-dependent, step-by-step intron-loss" evolution.The evolutionary feature of globin and hatching enzyme genes reflects a characteristic of Teleost such that they could adapt in extremely diversified environments during evolution.

本研究阐明了青鳉珠蛋白基因结构和表达的三个特点：1）α和β珠蛋白基因的头对头定位; 2）在各自基因上游1 Kb-5 '端存在负责基因时空表达的顺式调控区; 3）珠蛋白基因在2条染色体上定位于2个簇。分子系统发育分析表明，硬骨鱼类的珠蛋白基因可分为4类，其中1类的成年珠蛋白基因在进化过程中被假基因化。此外，我们还在30种硬骨鱼基因组中鉴定了孵化酶基因。结果表明，在鱼形目鱼类中，孵化酶基因具有祖先性，在耳头鱼和真骨鱼的后代中，孵化酶基因分化为2组。珠蛋白和孵化酶基因的进化特征反映了硬骨鱼科鱼类在进化过程中适应环境极其多样化的特点。

项目成果

Globin gene enhancer activity of a DNase-I hypersensitive site-40homolog in medaka,Oryzias latipes

青鳉 DNase-I 超敏位点 40 同源物的球蛋白基因增强子活性

• 发表时间: 2007
• 期刊: Zoolog Sci 24
• 作者: Maruyama K.;Ishikawa Y.;Yasumasu S.;Iuchi I.
• 通讯作者: Iuchi I.

卵胎生魚クロソイの孵化酵素LCE遺伝子における偽遺伝子形成過程

卵胎生鱼Kurosoi孵化酶LCE基因假基因的形成过程

• 发表时间: 2007
• 作者: 川口 眞理;安増 茂樹;廣井 準也;中川 雅弘;野田 勉;井内 一郎
• 通讯作者: 井内 一郎

Purification and gene cloning of Fundulus heteroclitus hatching enzyme : A hatching enzyme system composed of high choriolytic enzyme(HCE) and low choriolytic enzyme(LCE) is conserved between two different teleosts, F.heteroclitus and medaka Oryzias latip

异斜眼底孵化酶的纯化和基因克隆：两种不同的硬骨鱼F.heteroclitus和青鳉之间保守了由高绒毛膜溶解酶(HCE)和低绒毛膜溶解酶(LCE)组成的孵化酶系统。

• 发表时间: 2005
• 期刊: FEBS J. 272
• 作者: Kawaguchi;M.;Yasumasu;S.;Shimizu;A.;Hiroi;J.;Yoshizaki;N.;Nagata;K.;Tanokura;M.;luchi;I.
• 通讯作者: I.

Analysis of the exon-intron structures of fish, amphibian, bird and mammalian hatching enzyme genes, with special reference to the intron loss evolution of hatching enzyme genes in Teleostei

• DOI: 10.1016/j.gene.2006.11.012
• 发表时间: 2007-05-01
• 期刊: GENE
• 影响因子: 3.5
• 作者: Kawaguchi, Marl;Yasumasu, Shigeki;Iuchi, Ichiro
• 通讯作者: Iuchi, Ichiro

Evolution of teleostean hatching enzyme genes and their paralogous genes

• DOI: 10.1007/s00427-006-0104-5
• 发表时间: 2006-12-01
• 期刊: DEVELOPMENT GENES AND EVOLUTION
• 影响因子: 2.4
• 作者: Kawaguchi, Mari;Yasumasu, Shigeki;Iuchi, Ichiro
• 通讯作者: Iuchi, Ichiro