Are rare earth elements essential for growth of microorganisms?

稀土元素对微生物的生长至关重要吗？

基本信息

批准号：
17580063
负责人：
KAWAI Keiichi
金额：
$ 2.59万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The rare earth elements have been used for various high technology products. We have been screening microorganisms which show a peculiar growth in the presence of rare earth elements. We had isolated successfully some rare-earth responsive microorganisras(sliains EU-1, EU-2 and EU-3). In thisresearch, we investigated on three strains (EU-1, EU-2 and EU-3) which formed the bigger colony size in the presence of europium (Eu). Strain EU-1 was identified as Methylobactetizun sp. The growthof this strain was stimulated by Sm on 1/100 nutrient agar plate with methanol. The growth of this bacterium increased about 10-times when Sm was added to the liquid medium, suggesting that Sm was involved in the methanol metabolism of this bacterium. Strain EU-2 was identified as Nevskia ramosea. The growth of strain EU-2 was found to be increased by Eu on a soft agar: This indicated that the mobility of this bacterium was controlled by Eu. Although the viable cells were increased 20 times by adding Eu t … More o the medium, some dispersion of the colony munber appeared on the plate was found. This was due to the aggregation of the cells especially in the absence of Eu Strain EU-3, identified as Sphingomonas sp. EU-3, when the cell growth was reached to stationary phase, immediately the cells went into the death phase. La addition to the medium, however, the stationary phase in was continued. La have uhvsiolotlically important role in survival of strain EU-3.Methylobactelium sp.EU-1 was cultivated in the medium containing methanol and light rare earth elements, activity of methanol dehydrogenase in crude extracts of Afethylkobacterium sp. EU-1 was markedly increased. Especially La or Ce caused the increment of methanol dehydrogenase activity about 150 times. The dehydrogenase was purified.The purified enzyme migrated as a single band on SDS-PAGE. The N-terminal amino acid sequence of the dehydrogen-ase showed the high similarity with methanol dehydrogenase or PQQ-dependent dehydrogenase of Methylobacteiitun spp. Furthermore, the absorption spectra of the dehydrogenase revealed that this dehydrogenase was a member of PQQ-dependent dehydrogenase family.These results indicated a biological importance both in viva and in vitro of rare earth elements and will give a great impact to the fields of bioinorganic chemistry. Less

稀土元素已用于各种高科技产品。我们一直在筛选微生物，这些微生物在存在稀土元素的情况下显示出奇特的生长。我们已经成功地分离了一些稀土反应式微组织（Sliains EU-1，EU-2和EU-3）。在此研究中，我们研究了三种菌株（EU-1，EU-2和EU-3），它们在存在Europium（EU）的情况下形成了较大的菌落大小。 EU-1菌株被鉴定为甲基二乙烯sp。 SM在用甲醇的1/100营养琼脂板上刺激了这种菌株的生长。当将SM添加到液体培养基中时，该细菌的生长大约增加了10倍，这表明SM参与该细菌的甲醇代谢。 EU-2菌株被确定为Nevskia ramosea。欧盟在软琼脂上发现了欧盟2菌株的生长：这表明该细菌的迁移率由欧盟控制。尽管通过添加Eu t…更多的培养基增加了20倍，但发现了菌落木结构的某些分散。这是由于细胞的聚集，尤其是在没有欧盟菌株EU-3的情况下，被确定为鞘氨拟补人sp。 EU-3，当将细胞生长到达固定阶段时，细胞立即进入死亡阶段。但是，在培养基中增加了固定阶段。 LA在菌株Eu-3.Methylobactelium sp.EU-1中具有UHVSiolotlotlotlotlatlastllotlotlotlastl的重要作用，在含有甲醇和光稀土元素的培养基中，甲基诺醇脱氢酶在Afethylkobacterium sp.eu-1的粗提取物中的活性显着增加。纯化脱氢酶。纯化的酶在SDS-PAGE上迁移为单个频带。脱氢基因酶的N末端氨基酸序列与甲基苯二醇脱氢酶或甲基bacteiitun spp的PQQ依赖性脱氢酶的高相似性。此外，脱氢酶的吸收光谱表明该脱氢酶是PQQ依赖性脱氢酶家族的成员。这些结果表明，体内和体外的稀土元素都具有生物学重要性，并将对生物无机化学领域产生重大影响。较少的

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effect of Rare Earth Elements on Growth Behaviors of Microorganisms

稀土元素对微生物生长行为的影响

DOI：
发表时间：
2005
期刊：
Microorganisms and Human Well-Being (査読無)
影响因子：
0
作者：
K. Kawai;T. Suzuki;T. Iwama
通讯作者：
T. Iwama

The rare eairth, scandium, causes antibiotic overproduction by Streptomyces spp.

稀有金属钪会导致链霉菌产生抗生素过量。

DOI：
发表时间：
2007
期刊：
FEMS Microbiol. Lett. 274
影响因子：
0
作者：
Kawai K.;Wang G.;Okamoto S. & Ochi K.,
通讯作者：
Okamoto S. & Ochi K.,

The rare earth, scandium, causes antibiotic overproduction in Streptoznyces spp

稀土钪导致链霉菌属抗生素过量生产

DOI：
发表时间：
2007
期刊：
FEMS Microbiol Lett.(査読有) 274
影响因子：
0
作者：
Aoki;N.;Matsuda;T.;K. Kawai;K. Kawai
通讯作者：
K. Kawai

Proteome Analysis of Bradyrhizobium sp. CE-3 in the Presence and Absenc of Ce or Mannitol

慢生根瘤菌的蛋白质组分析。

DOI：
发表时间：
2006
期刊：
Symposium on Panascopic Asemling and Highly Ordered Functions for Rare Earth Materials
影响因子：
0
作者：
Mako Fushimi;Michiko Hiraoka;Keiichi Kawai;et al.
通讯作者：
et al.

Growth behavior of Methylobacterium sp. EU-1 in the presence of Sm

甲基杆菌属的生长行为。