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Are rare earth elements essential for growth of microorganisms?

稀土元素对微生物的生长至关重要吗？

基本信息

批准号：
17580063
负责人：
KAWAI Keiichi
金额：
$ 2.59万
依托单位：
Gifu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

项目摘要

The rare earth elements have been used for various high technology products. We have been screening microorganisms which show a peculiar growth in the presence of rare earth elements. We had isolated successfully some rare-earth responsive microorganisras(sliains EU-1, EU-2 and EU-3). In thisresearch, we investigated on three strains (EU-1, EU-2 and EU-3) which formed the bigger colony size in the presence of europium (Eu). Strain EU-1 was identified as Methylobactetizun sp. The growthof this strain was stimulated by Sm on 1/100 nutrient agar plate with methanol. The growth of this bacterium increased about 10-times when Sm was added to the liquid medium, suggesting that Sm was involved in the methanol metabolism of this bacterium. Strain EU-2 was identified as Nevskia ramosea. The growth of strain EU-2 was found to be increased by Eu on a soft agar: This indicated that the mobility of this bacterium was controlled by Eu. Although the viable cells were increased 20 times by adding Eu t … More o the medium, some dispersion of the colony munber appeared on the plate was found. This was due to the aggregation of the cells especially in the absence of Eu Strain EU-3, identified as Sphingomonas sp. EU-3, when the cell growth was reached to stationary phase, immediately the cells went into the death phase. La addition to the medium, however, the stationary phase in was continued. La have uhvsiolotlically important role in survival of strain EU-3.Methylobactelium sp.EU-1 was cultivated in the medium containing methanol and light rare earth elements, activity of methanol dehydrogenase in crude extracts of Afethylkobacterium sp. EU-1 was markedly increased. Especially La or Ce caused the increment of methanol dehydrogenase activity about 150 times. The dehydrogenase was purified.The purified enzyme migrated as a single band on SDS-PAGE. The N-terminal amino acid sequence of the dehydrogen-ase showed the high similarity with methanol dehydrogenase or PQQ-dependent dehydrogenase of Methylobacteiitun spp. Furthermore, the absorption spectra of the dehydrogenase revealed that this dehydrogenase was a member of PQQ-dependent dehydrogenase family.These results indicated a biological importance both in viva and in vitro of rare earth elements and will give a great impact to the fields of bioinorganic chemistry. Less

稀土元素已被用于各种高科技产品。我们一直在筛选在稀土元素存在下表现出特殊生长的微生物。我们已经成功地分离出一些对稀土敏感的微生物（菌种EU-1、EU-2和EU-3）。在本研究中，我们研究了在铕（Eu）存在下形成较大菌落的3株菌株Eu -1、Eu -2和Eu -3。菌株EU-1鉴定为Methylobactetizun sp.菌株在1/100营养琼脂板上以甲醇为培养基，Sm刺激其生长。当Sm加入到液体培养基中时，该细菌的生长速度提高了约10倍，表明Sm参与了该细菌的甲醇代谢。菌株EU-2鉴定为拉莫斯奈夫氏菌。菌株Eu -2在软琼脂上的生长受到了Eu的促进，表明该菌的迁移受到了Eu的控制。在培养液中加入Eu使活菌数增加了20倍，但在培养液中发现菌落数量出现了一定的分散。这是由于细胞聚集，特别是在没有Eu菌株Eu -3（鉴定为Sphingomonas sp. Eu -3）的情况下，当细胞生长达到静止期时，细胞立即进入死亡期。然而，在加入介质后，固定相继续进行。La对菌株EU-3的存活具有非常重要的生理作用。在含甲醇和轻稀土元素的培养基中培养甲基杆菌sp.EU-1，其粗提物甲醇脱氢酶活性显著提高。特别是镧和铈使甲醇脱氢酶活性增加约150倍。对脱氢酶进行纯化。纯化后的酶在SDS-PAGE上呈单条带迁移。该脱氢酶的n端氨基酸序列与甲基杆菌的甲醇脱氢酶或pqq依赖性脱氢酶具有较高的相似性，并且该脱氢酶的吸收光谱显示该脱氢酶是pqq依赖性脱氢酶家族的成员。这些结果表明稀土元素在体内和体外都具有重要的生物学意义，并将对生物无机化学领域产生重大影响。少

项目成果

期刊论文数量（0）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Effect of Rare Earth Elements on Growth Behaviors of Microorganisms

稀土元素对微生物生长行为的影响

DOI：
发表时间：
2005
期刊：
Microorganisms and Human Well-Being (査読無)
影响因子：
0
作者：
K. Kawai;T. Suzuki;T. Iwama
通讯作者：
T. Iwama

The rare eairth, scandium, causes antibiotic overproduction by Streptomyces spp.

稀有金属钪会导致链霉菌产生抗生素过量。

DOI：
发表时间：
2007
期刊：
FEMS Microbiol. Lett. 274
影响因子：
0
作者：
Kawai K.;Wang G.;Okamoto S. & Ochi K.,
通讯作者：
Okamoto S. & Ochi K.,

The rare earth, scandium, causes antibiotic overproduction in Streptoznyces spp

稀土钪导致链霉菌属抗生素过量生产

DOI：
发表时间：
2007
期刊：
FEMS Microbiol Lett.(査読有) 274
影响因子：
0
作者：
Aoki;N.;Matsuda;T.;K. Kawai;K. Kawai
通讯作者：
K. Kawai

Proteome Analysis of Bradyrhizobium sp. CE-3 in the Presence and Absenc of Ce or Mannitol

慢生根瘤菌的蛋白质组分析。

DOI：
发表时间：
2006
期刊：
Symposium on Panascopic Asemling and Highly Ordered Functions for Rare Earth Materials
影响因子：
0
作者：
Mako Fushimi;Michiko Hiraoka;Keiichi Kawai;et al.
通讯作者：
et al.

Application of Rare Earths in Agriculture(in Japanese)

稀土在农业中的应用（日语）