Are rare earth elements essential for growth of microorganisms?
稀土元素对微生物的生长至关重要吗?
基本信息
- 批准号:17580063
- 负责人:
- 金额:$ 2.59万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2005
- 资助国家:日本
- 起止时间:2005 至 2007
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The rare earth elements have been used for various high technology products. We have been screening microorganisms which show a peculiar growth in the presence of rare earth elements. We had isolated successfully some rare-earth responsive microorganisras(sliains EU-1, EU-2 and EU-3). In thisresearch, we investigated on three strains (EU-1, EU-2 and EU-3) which formed the bigger colony size in the presence of europium (Eu). Strain EU-1 was identified as Methylobactetizun sp. The growthof this strain was stimulated by Sm on 1/100 nutrient agar plate with methanol. The growth of this bacterium increased about 10-times when Sm was added to the liquid medium, suggesting that Sm was involved in the methanol metabolism of this bacterium. Strain EU-2 was identified as Nevskia ramosea. The growth of strain EU-2 was found to be increased by Eu on a soft agar: This indicated that the mobility of this bacterium was controlled by Eu. Although the viable cells were increased 20 times by adding Eu t … More o the medium, some dispersion of the colony munber appeared on the plate was found. This was due to the aggregation of the cells especially in the absence of Eu Strain EU-3, identified as Sphingomonas sp. EU-3, when the cell growth was reached to stationary phase, immediately the cells went into the death phase. La addition to the medium, however, the stationary phase in was continued. La have uhvsiolotlically important role in survival of strain EU-3.Methylobactelium sp.EU-1 was cultivated in the medium containing methanol and light rare earth elements, activity of methanol dehydrogenase in crude extracts of Afethylkobacterium sp. EU-1 was markedly increased. Especially La or Ce caused the increment of methanol dehydrogenase activity about 150 times. The dehydrogenase was purified.The purified enzyme migrated as a single band on SDS-PAGE. The N-terminal amino acid sequence of the dehydrogen-ase showed the high similarity with methanol dehydrogenase or PQQ-dependent dehydrogenase of Methylobacteiitun spp. Furthermore, the absorption spectra of the dehydrogenase revealed that this dehydrogenase was a member of PQQ-dependent dehydrogenase family.These results indicated a biological importance both in viva and in vitro of rare earth elements and will give a great impact to the fields of bioinorganic chemistry. Less
稀土元素已被用于各种高科技产品。我们一直在筛选在稀土元素存在下表现出特殊生长的微生物。成功地分离出了几株稀土敏感微生物(菌株EU-1、EU-2和EU-3)。本研究对在铕(Eu)存在下形成较大菌落的3株细菌(EU-1、EU-2和EU-3)进行了研究。EU-1菌株经鉴定为甲基杆菌属(Methylobactetizun sp.)。当Sm加入到液体培养基中时,该细菌的生长增加了约10倍,表明Sm参与了该细菌的甲醇代谢。菌株EU-2经鉴定为多枝涅夫斯基菌(Nevskia ramosea)。在软琼脂培养基上,Eu对菌株EU-2的生长有促进作用,表明该菌的运动性受Eu的控制。虽然加入Eu后活细胞数增加了20倍, ...更多信息 在培养基中,发现平板上出现了一些分散的殖民地,这是由于细胞聚集所致,尤其是在缺乏Eu菌株EU-3的情况下,经鉴定为鞘氨醇单胞菌EU-3,当细胞生长达到稳定期时,细胞立即进入死亡期。然而,在培养基中加入La后,固定相仍在继续。La对EU-3菌株的存活具有重要的生理作用。甲基杆菌EU-1在含有甲醇和轻稀土元素的培养基中培养,其粗提物中的甲醇脱氢酶活性显著提高。特别是La或Ce可使甲醇脱氢酶活性提高约150倍。纯化的脱氢酶在SDS-PAGE上为单一条带。N-末端氨基酸序列与甲基杆菌属的甲醇脱氢酶或PQQ依赖性脱氢酶高度相似。此外,该脱氢酶的吸收光谱表明该脱氢酶属于PQQ依赖型脱氢酶家族,这些结果表明稀土元素在体内和体外都具有重要的生物学意义,并将对生物无机化学领域产生重大影响。少
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Effect of Rare Earth Elements on Growth Behaviors of Microorganisms
稀土元素对微生物生长行为的影响
- DOI:
- 发表时间:2005
- 期刊:
- 影响因子:0
- 作者:K. Kawai;T. Suzuki;T. Iwama
- 通讯作者:T. Iwama
The rare eairth, scandium, causes antibiotic overproduction by Streptomyces spp.
稀有金属钪会导致链霉菌产生抗生素过量。
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Kawai K.;Wang G.;Okamoto S. & Ochi K.,
- 通讯作者:Okamoto S. & Ochi K.,
The rare earth, scandium, causes antibiotic overproduction in Streptoznyces spp
稀土钪导致链霉菌属抗生素过量生产
- DOI:
- 发表时间:2007
- 期刊:
- 影响因子:0
- 作者:Aoki;N.;Matsuda;T.;K. Kawai;K. Kawai
- 通讯作者:K. Kawai
Proteome Analysis of Bradyrhizobium sp. CE-3 in the Presence and Absenc of Ce or Mannitol
慢生根瘤菌的蛋白质组分析。
- DOI:
- 发表时间:2006
- 期刊:
- 影响因子:0
- 作者:Mako Fushimi;Michiko Hiraoka;Keiichi Kawai;et al.
- 通讯作者:et al.
Application of Rare Earths in Agriculture(in Japanese)
稀土在农业中的应用(日语)
- DOI:
- 发表时间:2008
- 期刊:
- 影响因子:0
- 作者:Aoki;N.;Matsuda;T.;K. Kawai
- 通讯作者:K. Kawai
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KAWAI Keiichi其他文献
KAWAI Keiichi的其他文献
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{{ truncateString('KAWAI Keiichi', 18)}}的其他基金
Development strategy of tumor diagnostic agent by system upregulated functional biomolecule based on expression analysis of tumor associated transporter
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15K15452 - 财政年份:2015
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$ 2.59万 - 项目类别:
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Development of a molecular targeted radiolabeled diagnostic agent useful for personalized drug therapy of psychoneurotic diseases
开发可用于精神神经疾病个性化药物治疗的分子靶向放射性标记诊断剂
- 批准号:
25293260 - 财政年份:2013
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$ 2.59万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
The design strategy for tumor diagnostic agents utilizing the functional biomolecule expression system based on gene expression analysis of tumor cells
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- 批准号:
24659558 - 财政年份:2012
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$ 2.59万 - 项目类别:
Grant-in-Aid for Challenging Exploratory Research
The probe design strategy of post-FDG tumor diagnostic agents based on the functional biomolecule expression analysis of human cultured cells
基于人培养细胞功能生物分子表达分析的FDG后肿瘤诊断剂探针设计策略
- 批准号:
21659286 - 财政年份:2009
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Grant-in-Aid for Challenging Exploratory Research
Development of novel radiopharmaceuticals for diagnosis of cerebral neurodegeneration and functional recovery after intrastriatal grafts/gene therapy
开发用于诊断脑神经变性和纹状体移植/基因治疗后功能恢复的新型放射性药物
- 批准号:
20249055 - 财政年份:2008
- 资助金额:
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Grant-in-Aid for Scientific Research (A)
Are rare earth elements essential for growth of methylotrophs?
稀土元素对于甲基营养菌的生长至关重要吗?
- 批准号:
20580075 - 财政年份:2008
- 资助金额:
$ 2.59万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
DEVELOPMENT OF NOVEL RADIOPHARMACEUTICALS FOR EARLY STAGE DIAGNOSIS OF CEREBRAL NEURODEGENERATION AND FUNCTIONAL RECOVERRY AFTER INTRASTRIATAL GRAFTS
开发用于脑神经变性早期诊断和纹状移植后功能恢复的新型放射性药物
- 批准号:
17390329 - 财政年份:2005
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Grant-in-Aid for Scientific Research (B)
DEVELOPMENT OF NOVEL RADIOPHARMACEUTICALS FOR EARLY STAGE DIAGNOSIS OF CEREBRAL NEURODEGENERATION.
开发用于脑神经退行性早期诊断的新型放射性药物。
- 批准号:
14370273 - 财政年份:2002
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DEVELOPEMENT OF RADIOIODINATED RADIOPHARMACEUTICALS FOR INTERNAL RADIATION THERAPY OF TUMOR BASED ON ITS SPECIFIC METABOLIC ACTIVITY
基于肿瘤特异性代谢活性的用于肿瘤内放射治疗的放射性碘化放射性药物的开发
- 批准号:
12557076 - 财政年份:2000
- 资助金额:
$ 2.59万 - 项目类别:
Grant-in-Aid for Scientific Research (B)